LOCUS       sequence-205556-       10013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-337
FEATURES             Location/Qualifiers
     source          1..10013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     907..926
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(946..1023)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1229..1248
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1332..1351
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1332..1350
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1351..1375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1429..1446
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4994..5083
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5096..5185
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(6279..6297)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6293..6340
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6377..6832
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6464..6483)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6674..6695
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6924..7739)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6924..7739)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7647..7666
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7731..7750
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7861..8449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8350..8369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8603..8620
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8635..8774
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8700..8719)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8860..8882
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8876..9067)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9876..10013,1..945))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
       61 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcctcggt
      121 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc
      181 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
      241 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
      301 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
      361 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
      421 gaccagatgc tccacgccca gtcgcgtacc gtcctcatgg gagaaaataa tactgttgat
      481 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
      541 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
      601 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
      661 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
      721 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
      781 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
      841 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
      901 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
      961 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     1021 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     1081 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     1141 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     1201 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     1261 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
     1321 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
     1381 agaaataatt ttgccttaac tttaagaagg agatatacca tgggcagcca ccaccaccac
     1441 caccacagcg gaatgcagat ctttgttaag actctcaccg gaaagacaat caccctagag
     1501 gtggaaagct ccgacaccat cgacaacgtt aaggccaaga tccaggataa ggagggcatt
     1561 cctccggatc agcagaggct tattttcgcc ggcaagcagc tagaggatgg ccgtacgttg
     1621 gctgattaca atatccagaa ggaatccacc ctccacttgg tcctcaggct ccgtggtggt
     1681 taagcttgca attaacttta agaaggagat atacaatgct tcacaagcga gctagtgaag
     1741 ctaacgataa gaacgataat accatcatcg gttccgattt agcatcatct aagaaacgcc
     1801 gtatcgattt cactgaatct tcgtctgata agtcatcttc gattttagct tctggtagta
     1861 gcaggggttt tcacggcgat agcgtcgttc agcaaatcga catggctttt ggtaattcga
     1921 accgtcagga gattgatgaa gatctgcaca gtcgccagct cgccgtctat ggtcgtgaga
     1981 ctatgaggcg tctctttgct tccaatgttc tcatctcggg gatgcacggt cttggtgccg
     2041 agattgccaa gaatcttata cttgctggtg tgaagtctgt gaccttgcat gatgaaagag
     2101 ttgtagagct atgggactta tcaagcaact ttgttttctc ggaggatgat gttggcaaga
     2161 atagagcaga tgcttctgtt cagaagttac aggatcttaa caatgctgtg gttgtctcta
     2221 gcttgaccaa aagcttaaac aaagaggatc tttctggctt ccaggttgtt gtattctctg
     2281 acataagcat ggaaagagca atcgaatttg atgactattg tcacagccat cagcctccta
     2341 tcgcttttgt caaggctgat gtcaggggtc ttttcggctc tgtcttttgt gattttgggc
     2401 ctgaatttgc ggttcttgat gttgatggag aggaacccca tacaggcatc attgcgtcta
     2461 tctctaatga gaaccaggca tttatttcct gtgtcgacga tgagaggctt gagtttgaag
     2521 atggtgacct agttgttttc tcagaagttg agggtatgac agaattgaat gatgggaaac
     2581 caaggaagat aaaaagtaca cggccatatt cattcaccct tgatgaggac actactaact
     2641 atggaacata tgtgaagggt ggtattgtca ctcaagtgaa acagccaaag ttactgaatt
     2701 tcaagccatt gagggaagcg cttaaggatc caggggattt tctatttagt gatttctcta
     2761 agtttgatcg gcctccactc cttcacttag cattccaggc acttgatcat tttaaagctg
     2821 aagctggcag atttcctgtt gctggctcag aagaggacgc tcagaagctt atatcgattg
     2881 ccacagccat caataccggg cagggtgatt taaaggtgga gaatgttgat cagaagcttc
     2941 taagacactt ttcctttgga gccaaggctg tcctgaatcc tatggctgca atgtttggcg
     3001 gtattgttgg acaggaggtt gttaaagctt gctctggaaa atttcatcct ctcttccagt
     3061 ttttctactt tgattcagtg gagtcactcc cctctgaacc tgtggactcg agtgactttg
     3121 caccaagaaa cagccggtat gatgcccaaa tatctgtatt tggggccaag ttccagaaga
     3181 aacttgaaga tgctaaagtt ttcacagtag ggtctggtgc tcttggctgt gagttcttga
     3241 aaaatttggc tctgatgggg gtttcttgtg gaagccaagg gaagctgaca gtgacagatg
     3301 atgatatcat tgagaagagt aacctcagtc gtcaatttct gttccgtgac tggaacattg
     3361 gacaggctaa atccacagtt gctgcttcgg ctgctgctgt tataaatcct cgcttcaaca
     3421 ttgaggccct gcaaaaccgt gttggcgcgg agactgagaa tgtatttgac gatgccttct
     3481 gggagaactt aactgttgtc gtcaatgcac tggataatgt taatgcgagg ctctatgttg
     3541 attcgaggtg cttgtatttc cagaagcctc tccttgagtc tggaactctt ggtacaaagt
     3601 gcaacacaca gtcggttatc ccacatctaa ctgaaaatta cggtgcctca agggacccac
     3661 cagagaaaca ggcccccatg tgtacagtgc attccttccc gcataacatt gatcactgtt
     3721 taacttgggc tcgctctgag tttgagggtc tgctggagaa aactcccgct gaagtgaatg
     3781 catatctttc tagcccagtt gagtacacta actcaatgat gagtgctggt gatgctcagg
     3841 cgagggacac attggagagg attgttgagt gccttgaaaa ggagaagtgt gaaaccttcc
     3901 aagactgctt aacctgggca cgactgaggt ttgaggatta ctttgtgaac cgtgtcaagc
     3961 aattgatata cacatttcct gaagatgctg cgactagtac cggagctcca ttctggtctg
     4021 ctccaaaaag attccctcgt ccactccagt actcgtcgtc cgacccaagt ctccttaact
     4081 ttatcacagc gactgctatt ttacgagcag agacatttgg gatcccgata cctgagtgga
     4141 ctaagaaccc aaaggaagca gcagaagctg ttgacagagt gatagtccca gactttgagc
     4201 caaggcaaga tgcaaagatt gtgactgatg agaaagctac cactttaacc actgcttcgg
     4261 tggacgacgc tgcagtcatc gacgatctca ttgctaagat cgaccagtgt aggcataact
     4321 tgtcgccaga cttcaggatg aaacctattc agttcgaaaa ggatgatgat acaaactatc
     4381 acatggatgt gatagcagga ctagccaaca tgagggcgag aaattacagc atccctgaag
     4441 tggacaagct gaaagcaaag ttcatagcag ggagaatcat cccagccatt gcgacttcaa
     4501 cagccatggc tactgggctg gtctgcctcg agctgtacaa ggtccttgat ggaggacata
     4561 aagtggaagc ttacagaaac acatttgcca acctggcact tccactattc tccatggctg
     4621 aaccacttcc accaaaggtg gtgaagcacc gtgacatggc ttggacagtt tgggacagat
     4681 gggttctcaa gggaaaccca actcttcgtg aggtgttgca gtggctggag gacaaagggc
     4741 ttagcgcata cagcatctct tgtggaagct gtctactgtt caacagtatg ttcacaaggc
     4801 acaaggagag gatggacaag aaggttgtgg atctcgctcg ggatgttgcg aaagtcgagt
     4861 taccgcctta tcgcaaccat cttgatgtag tggtggcttg tgaggatgaa gatgacaatg
     4921 acgtcgatat tcctctcgtc tctatctact tcaggtgagc ttactattaa ctttaagaag
     4981 gagatatacc atgtacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
     5041 ggactatgca ggattgtatc catatgacgt tccagattac gccactagag ctgcttaccc
     5101 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggattgta
     5161 tccatatgac gttccagatt acgcaatggc caatagtaat cttccccgac gaatcatcaa
     5221 ggaaactcaa cgtctgctta gtgaacctgc tcctgggata agtgcgtctc catcagagga
     5281 aaatatgaga tatttcaatg ttatgattct tggtcctact caatctcctt atgaaggagg
     5341 agttttcaag ttggagctct ttttgcctga agaatatcct atggcagctc ccaaggttag
     5401 gtttctcacc aagatttacc atcctaacat cgacaagctt ggaagaatct gccttgacat
     5461 actgaaagac aagtggagtc ctgcactaca aatacgaact gtgctcttga gtatccaagc
     5521 tcttttaagt gcaccaaacc cagatgatcc tctgtctgag aacatagcta agcattggaa
     5581 gagcaatgag gcagaagctg tggaaacagc taaggaatgg actcgtcttt acgcaagtgg
     5641 tgcttaagct tttacttaac tttaagaagg agatatacaa tgactcttgg ctcaggatcg
     5701 agtgttgtcg ttccgaggaa tttccggttg ctggaggagc ttgaacgtgg agagaaaggt
     5761 attggagatg gaactgtgag ctatggaatg gatgatggag atgacattta tatgcgctct
     5821 tggactggca ctatcatcgg tcctcacaac gtaactgtac atgagggtcg gatttatcag
     5881 ttgaagctct tctgtgacaa agattaccct gagaaacctc cgactgttcg gttccattcg
     5941 cgtatcaaca tgacttgtgt caaccatgat accggcgtgg tggattcaaa gaagtttggg
     6001 gttcttgcaa actggcaaag acagtacaca atggaggaca tactgactca attgaagaag
     6061 gagatggctg catcgcataa ccggaagctt gttcaacctc ccgaaggaac tttcttctaa
     6121 gcttcagagc ttttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     6181 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgggatgag atccggctgc
     6241 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     6301 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     6361 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6421 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6481 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6541 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6601 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6661 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6721 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6781 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6901 tgtatccgct catgaattaa ttcttagaaa aactcatcga gcatcaaatg aaactgcaat
     6961 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     7021 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     7081 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     7141 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct
     7201 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     7261 aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa atacgcgatc gctgttaaaa
     7321 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     7381 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
     7441 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     7501 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     7561 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     7621 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     7681 tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata
     7741 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga ccaaaatccc
     7801 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7861 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7921 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7981 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     8041 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8101 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8161 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8221 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8281 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8341 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8401 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8461 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8521 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8581 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     8641 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
     8701 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     8761 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     8821 tgctcccggc atccgcttac agacaagctg tgaccgactc cgggagctgc atgtgtcaga
     8881 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     8941 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     9001 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     9061 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     9121 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     9181 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     9241 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     9301 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     9361 acgaaacacg gaaacccaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     9421 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     9481 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     9541 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     9601 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     9661 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     9721 cgagttgcat gataaagaac acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     9781 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     9841 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9961 ttgggcgcca gggtggtttt tcttttcacc agtgacacgg gcaacagctg att
//
