LOCUS       sequence-205557-       11481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation A169D conferring gain of
            function phenotype, decreased surface expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11481)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTA169D-myc
FEATURES             Location/Qualifiers
     source          1..11481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(692..710)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        778..882
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(883..951,952..1743)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1101..1120)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1914..2502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2403..2422
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2933..3078
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3121..4158
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4156..4175)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4200..4248
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4902..5281
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5282..5485
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5436..5456
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5482..5506
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5534..5543
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6209..6238
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7527..7661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7551..7570)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7605..7624
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7804..7826)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8386..10175
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac accatctcta
       61 tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc tcgtcctcgt
      121 cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc actgccgccg
      181 gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg tcctgtacct
      241 ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt attagacgac
      301 gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc caccctcatc
      361 cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct ccgaaccatc
      421 ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt
      481 tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag
      541 ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca gagatgctac
      601 tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg atgataagct
      661 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
      721 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      781 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      841 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      901 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      961 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1021 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1081 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     1141 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1201 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1261 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1321 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1381 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     1441 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1501 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1561 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1621 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     1681 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1741 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1801 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1861 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1921 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1981 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2041 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2101 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2161 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2221 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2281 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2341 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2401 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2461 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2521 tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc gtcatctacc
     2581 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     2641 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
     2701 tcggccccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct
     2761 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
     2821 gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc gttgctcgcg
     2881 tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca
     2941 aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg
     3001 cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc
     3061 ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg gcaatgagat
     3121 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     3181 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     3241 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     3301 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     3361 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     3421 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     3481 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     3541 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     3601 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     3661 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     3721 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     3781 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     3841 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     3901 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     3961 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     4021 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     4081 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc ggatcgggag
     4141 atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     4201 ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg
     4261 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     4321 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     4381 cagccaacgt cggggcggca ggccctgcca tagccactgg ccccgtgggt tagggacggg
     4441 gtcccccatg gggaatggtt tatggttcgt gggggttatt attttgggcg ttgcgtgggg
     4501 tcaggtccac gactggactg agcagacaga cccatggttt ttggatggcc tgggcatgga
     4561 ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc
     4621 ccaaaaacca ccgcgcggat ttctggcgtg ccaagctagt cgaccaattc tcatgtttga
     4681 cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta
     4741 ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta
     4801 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
     4861 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
     4921 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     4981 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     5041 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     5101 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     5161 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     5221 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     5281 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
     5341 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     5401 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
     5461 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatctcta gaagctgggt
     5521 accagctgct agcgccacca tggagaccac ccccctgaac agtcaaaagc aactctctgc
     5581 ctgtgaggat ggagaggact gtcaggagaa tggcgttctt caaaaggttg tacccacccc
     5641 tggagataag gtggaaagcg gccagatttc caacggctac agcgccgtgc catcacctgg
     5701 agccggggat gatacacggc acagcattcc cgcaaccacc acgacactgg ttgcagagct
     5761 tcaccagggc gagagggaga cgtggggcaa aaaggtagat tttctgcttt ccgtgatcgg
     5821 ttacgcggtc gatctgggga acgtgtggag gttcccttac atttgttacc aaaacggagg
     5881 gggtgccttc ttgctgccct acacaattat ggcgattttt gggggtatcc cgctcttcta
     5941 catggaactg gccttgggtc agtaccaccg gaacggctgc attagcattt ggcgcaagat
     6001 atgccccatt ttcaagggaa tcggatacgc aatctgtata atcgacttct atattgcatc
     6061 atattacaac accatcatgg cctgggccct ttattacttg atcagcagtt tcaccgacca
     6121 attgccgtgg acgagctgta agaactcatg gaataccggg aactgtacca actacttctc
     6181 tgaggacagc tcaggaggta gcgggggtga acagaagctg attagcgagg aggacttggg
     6241 cagtccaggc ggaagcggca acatcacttg gacactccat agtacgagcc ccgcagagga
     6301 gttttacacc aggcacgtac tgcagattca taggagcaag ggtctgcaag acctgggcgg
     6361 tattagctgg cagctcgcat tgtgcataat gttgatcttt acggtgatct atttctctat
     6421 ctggaaggga gtaaagacat ccggcaaggt ggtgtgggtg acggcgacct tcccctacat
     6481 tatactcagc gtgttgttgg tgaggggggc aaccctgcct ggggcctggc gaggcgtgct
     6541 gttctatctc aagccaaact ggcagaagct tcttgaaacc ggagtgtgga tcgacgcggc
     6601 cgcgcaaatc ttcttttccc tcggtcccgg cttcggggtc cttctggcat tcgcaagcta
     6661 caacaagttc aacaataact gctaccagga cgccctcgtg acaagtgtag tgaactgcat
     6721 gacctctttc gtgagcgggt ttgtgatctt cacagtactc ggatatatgg ctgagatgag
     6781 gaacgaagat gttagtgagg ttgccaaaga cgctggcccg agcctgttgt tcatcacata
     6841 cgccgaggct atcgcgaaca tgcccgcatc tactttcttc gccatcatct ttttcctgat
     6901 gctgataacc cttgggctgg atagcacctt cgccggactg gagggtgtca tcactgccgt
     6961 tctcgacgag ttcccccatg tgtgggccaa gcgacgggaa aggttcgtgc tggccgtcgt
     7021 aatcacatgc ttcttcggct cactggtgac ccttaccttt ggcggtgcgt acgtggtcaa
     7081 gctgctcgaa gagtacgcca ctggaccggc ggttctgacc gtggcattga tcgaggccgt
     7141 ggccgtaagc tggttttacg gcatcacgca gttctgtagg gacgtgaaag agatgctggg
     7201 cttttctccc ggttggtttt ggaggatttg ttgggtagca attagccccc tgtttctcct
     7261 tttcattatt tgctcttttc tgatgtctcc accgcagctc cgactgtttc agtacaacta
     7321 cccctactgg tcaattatcc tgggctattg tatcggcacc tcaagtttca tctgcatccc
     7381 cacatacatc gcatacagac tgataattac gcccggtact tttaaggagc gaatcattaa
     7441 aagcataacc cccgaaaccc ccaccgagat cccttgtggc gatataagac tcaatgcggt
     7501 ctaatagctc gaggccggca aggccggatc cagacatgat aagatacatt gatgagtttg
     7561 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     7621 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     7681 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     7741 acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga
     7801 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     7861 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     7921 gaggtcgact ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct
     7981 ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca
     8041 actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact
     8101 gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct
     8161 ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg taactcttgg
     8221 ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg
     8281 ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa
     8341 gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg
     8401 cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc
     8461 aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat
     8521 ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag
     8581 tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc
     8641 tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg
     8701 tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat
     8761 aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca
     8821 aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata
     8881 gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg
     8941 gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca
     9001 ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag
     9061 agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg
     9121 gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt
     9181 gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata
     9241 agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc
     9301 acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata
     9361 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc
     9421 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc
     9481 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     9541 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     9601 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     9661 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     9721 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg
     9781 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     9841 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     9901 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     9961 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
    10021 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag
    10081 catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa
    10141 gggggcgtga attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa
    10201 tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta
    10261 atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat
    10321 gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca
    10381 ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct
    10441 tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc
    10501 atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc
    10561 caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac
    10621 cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa
    10681 aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct
    10741 tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg
    10801 tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt
    10861 cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc
    10921 tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt
    10981 ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc
    11041 accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc
    11101 tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa
    11161 ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg
    11221 tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc
    11281 acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc
    11341 ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc
    11401 actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc
    11461 tcctgctcct gcccctcctg c
//
