LOCUS       sequence-205560-       11512 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation A331T conferring gain of
            function phenotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTA331T-myc
FEATURES             Location/Qualifiers
     source          1..11512
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(705..723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        791..895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(896..964,965..1756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1114..1133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1927..2515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2416..2435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2946..3091
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3134..4171
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4169..4188)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4213..4261
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4915..5294
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5295..5498
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5449..5469
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5495..5519
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5547..5556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6222..6251
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7540..7674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7564..7583)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7618..7637
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7817..7839)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8399..10188
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt ggggtctccg
       61 gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg gtcttccgcc
      121 tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc ttctttgagg
      181 tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta ggccatttcc
      241 aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc aatagacatc
      301 tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct ccaacagccc
      361 ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa aattccccat
      421 cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa
      481 catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg tccccctttt
      541 tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca aggtcaccag
      601 acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag gatcagctta
      661 tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct
      721 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1081 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     1141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     1441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1741 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1801 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1861 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2281 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2341 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2401 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2461 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2521 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct gtccctgatg
     2581 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
     2641 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac
     2701 gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg
     2761 atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg
     2821 gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc atccccgtgg
     2881 cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt
     2941 ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc
     3001 agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt gtggcctcga
     3061 acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg cagatcccgg
     3121 ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     3181 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     3241 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     3301 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     3361 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     3421 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     3481 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     3541 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     3601 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     3661 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     3721 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     3781 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     3841 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     3901 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     3961 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     4021 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     4081 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     4141 tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4201 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
     4261 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4321 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     4381 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg
     4441 ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg
     4501 gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg tttttggatg
     4561 gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa
     4621 acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct agtcgaccaa
     4681 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     4741 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     4801 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     4861 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca tgttgacatt
     4921 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     4981 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     5041 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     5101 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     5161 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     5221 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
     5281 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
     5341 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     5401 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
     5461 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatct
     5521 ctagaagctg ggtaccagct gctagcgcca ccatggagac cacccccctg aacagtcaaa
     5581 agcaactctc tgcctgtgag gatggagagg actgtcagga gaatggcgtt cttcaaaagg
     5641 ttgtacccac ccctggagat aaggtggaaa gcggccagat ttccaacggc tacagcgccg
     5701 tgccatcacc tggagccggg gatgatacac ggcacagcat tcccgcaacc accacgacac
     5761 tggttgcaga gcttcaccag ggcgagaggg agacgtgggg caaaaaggta gattttctgc
     5821 tttccgtgat cggttacgcg gtcgatctgg ggaacgtgtg gaggttccct tacatttgtt
     5881 accaaaacgg agggggtgcc ttcttgctgc cctacacaat tatggcgatt tttgggggta
     5941 tcccgctctt ctacatggaa ctggccttgg gtcagtacca ccggaacggc tgcattagca
     6001 tttggcgcaa gatatgcccc attttcaagg gaatcggata cgcaatctgt ataatcgcgt
     6061 tctatattgc atcatattac aacaccatca tggcctgggc cctttattac ttgatcagca
     6121 gtttcaccga ccaattgccg tggacgagct gtaagaactc atggaatacc gggaactgta
     6181 ccaactactt ctctgaggac agctcaggag gtagcggggg tgaacagaag ctgattagcg
     6241 aggaggactt gggcagtcca ggcggaagcg gcaacatcac ttggacactc catagtacga
     6301 gccccgcaga ggagttttac accaggcacg tactgcagat tcataggagc aagggtctgc
     6361 aagacctggg cggtattagc tggcagctcg cattgtgcat aatgttgatc tttacggtga
     6421 tctatttctc tatctggaag ggagtaaaga catccggcaa ggtggtgtgg gtgacggcga
     6481 ccttccccta cattatactc agcgtgttgt tggtgagggg ggcaaccctg cctggggcct
     6541 ggcgaggcgt gctgttctat ctcaagccaa actggcagaa gcttcttgaa accggagtgt
     6601 ggatcgacgc ggccacgcaa atcttctttt ccctcggtcc cggcttcggg gtccttctgg
     6661 cattcgcaag ctacaacaag ttcaacaata actgctacca ggacgccctc gtgacaagtg
     6721 tagtgaactg catgacctct ttcgtgagcg ggtttgtgat cttcacagta ctcggatata
     6781 tggctgagat gaggaacgaa gatgttagtg aggttgccaa agacgctggc ccgagcctgt
     6841 tgttcatcac atacgccgag gctatcgcga acatgcccgc atctactttc ttcgccatca
     6901 tctttttcct gatgctgata acccttgggc tggatagcac cttcgccgga ctggagggtg
     6961 tcatcactgc cgttctcgac gagttccccc atgtgtgggc caagcgacgg gaaaggttcg
     7021 tgctggccgt cgtaatcaca tgcttcttcg gctcactggt gacccttacc tttggcggtg
     7081 cgtacgtggt caagctgctc gaagagtacg ccactggacc ggcggttctg accgtggcat
     7141 tgatcgaggc cgtggccgta agctggtttt acggcatcac gcagttctgt agggacgtga
     7201 aagagatgct gggcttttct cccggttggt tttggaggat ttgttgggta gcaattagcc
     7261 ccctgtttct ccttttcatt atttgctctt ttctgatgtc tccaccgcag ctccgactgt
     7321 ttcagtacaa ctacccctac tggtcaatta tcctgggcta ttgtatcggc acctcaagtt
     7381 tcatctgcat ccccacatac atcgcataca gactgataat tacgcccggt acttttaagg
     7441 agcgaatcat taaaagcata acccccgaaa cccccaccga gatcccttgt ggcgatataa
     7501 gactcaatgc ggtctaatag ctcgaggccg gcaaggccgg atccagacat gataagatac
     7561 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     7621 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     7681 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     7741 aagtaaaacc tctacaaatg tggtatggct gattatgatc cggctgcctc gcgcgtttcg
     7801 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     7861 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     7921 ggggcgcagc catgaggtcg actctagagg atcgatcccc gccccggacg aactaaacct
     7981 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     8041 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     8101 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     8161 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     8221 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     8281 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     8341 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     8401 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     8461 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     8521 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     8581 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     8641 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     8701 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     8761 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     8821 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     8881 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     8941 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     9001 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     9061 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     9121 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     9181 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     9241 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     9301 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     9361 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     9421 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     9481 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     9541 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     9601 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     9661 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     9721 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     9781 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     9841 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     9901 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     9961 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
    10021 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
    10081 catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct ttgcatatgc
    10141 cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct ggttgctccc
    10201 attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat tgctcaccag
    10261 gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct gagtgacgtg
    10321 acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga caagacagat
    10381 ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct ttagtgcggg
    10441 ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat cactcctgcc
    10501 cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca tcaccctccg
    10561 cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca tcgtcaaagc
    10621 tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca aggtccttaa
    10681 tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg aaataacaga
    10741 caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt ccaaagggga
    10801 gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct cgccttaggt
    10861 tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa gttccttcgt
    10921 cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct gcaatgttct
    10981 caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct ccttttttgc
    11041 gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct gggtcatctg
    11101 cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg ccaccttctt
    11161 ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt ctacctggag
    11221 ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc ctgggcctct
    11281 tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc ctggctcttt
    11341 cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca ctacctcctc
    11401 gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct gcccctcctg
    11461 ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gc
//
