LOCUS       sequence-205561-        9525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-352
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     969..988
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1008..1085)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1291..1310
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1394..1413
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1394..1412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1413..1437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1491..1508
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5056..5145
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5158..5247
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5853..5871)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5867..5914
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5951..6406
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6038..6057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6248..6269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6498..7313)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6498..7313)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7221..7240
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7305..7324
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7435..8023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7924..7943
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8177..8194
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8209..8348
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8274..8293)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8434..8456
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8450..8641)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9450..9525,1..1007))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgacacggg caacagctga
       61 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
      121 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcctcg
      181 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
      241 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
      301 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
      361 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
      421 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
      481 gcgaccagat gctccacgcc cagtcgcgta ccgtcctcat gggagaaaat aatactgttg
      541 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
      601 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
      661 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
      721 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
      781 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
      841 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
      901 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
      961 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
     1021 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
     1081 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     1141 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
     1201 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     1261 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     1321 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc
     1381 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
     1441 ctagaaataa ttttgcctta actttaagaa ggagatatac catgggcagc caccaccacc
     1501 accaccacag cggaatgcag atctttgtta agactctcac cggaaagaca atcaccctag
     1561 aggtggaaag ctccgacacc atcgacaacg ttaaggccaa gatccaggat aaggagggca
     1621 ttcctccgga tcagcagagg cttattttcg ccggcaagca gctagaggat ggccgtacgt
     1681 tggctgatta caatatccag aaggaatcca ccctccactt ggtcctcagg ctccgtggtg
     1741 gttaagcttg caattaactt taagaaggag atatacaatg cttcacaagc gagctagtga
     1801 agctaacgat aagaacgata ataccatcat cggttccgat ttagcatcat ctaagaaacg
     1861 ccgtatcgat ttcactgaat cttcgtctga taagtcatct tcgattttag cttctggtag
     1921 tagcaggggt tttcacggcg atagcgtcgt tcagcaaatc gacatggctt ttggtaattc
     1981 gaaccgtcag gagattgatg aagatctgca cagtcgccag ctcgccgtct atggtcgtga
     2041 gactatgagg cgtctctttg cttccaatgt tctcatctcg gggatgcacg gtcttggtgc
     2101 cgagattgcc aagaatctta tacttgctgg tgtgaagtct gtgaccttgc atgatgaaag
     2161 agttgtagag ctatgggact tatcaagcaa ctttgttttc tcggaggatg atgttggcaa
     2221 gaatagagca gatgcttctg ttcagaagtt acaggatctt aacaatgctg tggttgtctc
     2281 tagcttgacc aaaagcttaa acaaagagga tctttctggc ttccaggttg ttgtattctc
     2341 tgacataagc atggaaagag caatcgaatt tgatgactat tgtcacagcc atcagcctcc
     2401 tatcgctttt gtcaaggctg atgtcagggg tcttttcggc tctgtctttt gtgattttgg
     2461 gcctgaattt gcggttcttg atgttgatgg agaggaaccc catacaggca tcattgcgtc
     2521 tatctctaat gagaaccagg catttatttc ctgtgtcgac gatgagaggc ttgagtttga
     2581 agatggtgac ctagttgttt tctcagaagt tgagggtatg acagaattga atgatgggaa
     2641 accaaggaag ataaaaagta cacggccata ttcattcacc cttgatgagg acactactaa
     2701 ctatggaaca tatgtgaagg gtggtattgt cactcaagtg aaacagccaa agttactgaa
     2761 tttcaagcca ttgagggaag cgcttaagga tccaggggat tttctattta gtgatttctc
     2821 taagtttgat cggcctccac tccttcactt agcattccag gcacttgatc attttaaagc
     2881 tgaagctggc agatttcctg ttgctggctc agaagaggac gctcagaagc ttatatcgat
     2941 tgccacagcc atcaataccg ggcagggtga tttaaaggtg gagaatgttg atcagaagct
     3001 tctaagacac ttttcctttg gagccaaggc tgtcctgaat cctatggctg caatgtttgg
     3061 cggtattgtt ggacaggagg ttgttaaagc ttgctctgga aaatttcatc ctctcttcca
     3121 gtttttctac tttgattcag tggagtcact cccctctgaa cctgtggact cgagtgactt
     3181 tgcaccaaga aacagccggt atgatgccca aatatctgta tttggggcca agttccagaa
     3241 gaaacttgaa gatgctaaag ttttcacagt agggtctggt gctcttggct gtgagttctt
     3301 gaaaaatttg gctctgatgg gggtttcttg tggaagccaa gggaagctga cagtgacaga
     3361 tgatgatatc attgagaaga gtaacctcag tcgtcaattt ctgttccgtg actggaacat
     3421 tggacaggct aaatccacag ttgctgcttc ggctgctgct gttataaatc ctcgcttcaa
     3481 cattgaggcc ctgcaaaacc gtgttggcgc ggagactgag aatgtatttg acgatgcctt
     3541 ctgggagaac ttaactgttg tcgtcaatgc actggataat gttaatgcga ggctctatgt
     3601 tgattcgagg tgcttgtatt tccagaagcc tctccttgag tctggaactc ttggtacaaa
     3661 gtgcaacaca cagtcggtta tcccacatct aactgaaaat tacggtgcct caagggaccc
     3721 accagagaaa caggccccca tgtgtacagt gcattccttc ccgcataaca ttgatcactg
     3781 tttaacttgg gctcgctctg agtttgaggg tctgctggag aaaactcccg ctgaagtgaa
     3841 tgcatatctt tctagcccag ttgagtacac taactcaatg atgagtgctg gtgatgctca
     3901 ggcgagggac acattggaga ggattgttga gtgccttgaa aaggagaagt gtgaaacctt
     3961 ccaagactgc ttaacctggg cacgactgag gtttgaggat tactttgtga accgtgtcaa
     4021 gcaattgata tacacatttc ctgaagatgc tgcgactagt accggagctc cattctggtc
     4081 tgctccaaaa agattccctc gtccactcca gtactcgtcg tccgacccaa gtctccttaa
     4141 ctttatcaca gcgactgcta ttttacgagc agagacattt gggatcccga tacctgagtg
     4201 gactaagaac ccaaaggaag cagcagaagc tgttgacaga gtgatagtcc cagactttga
     4261 gccaaggcaa gatgcaaaga ttgtgactga tgagaaagct accactttaa ccactgcttc
     4321 ggtggacgac gctgcagtca tcgacgatct cattgctaag atcgaccagt gtaggcataa
     4381 cttgtcgcca gacttcagga tgaaacctat tcagttcgaa aaggatgatg atacaaacta
     4441 tcacatggat gtgatagcag gactagccaa catgagggcg agaaattaca gcatccctga
     4501 agtggacaag ctgaaagcaa agttcatagc agggagaatc atcccagcca ttgcgacttc
     4561 aacagccatg gctactgggc tggtctgcct cgagctgtac aaggtccttg atggaggaca
     4621 taaagtggaa gcttacagaa acacatttgc caacctggca cttccactat tctccatggc
     4681 tgaaccactt ccaccaaagg tggtgaagca ccgtgacatg gcttggacag tttgggacag
     4741 atgggttctc aagggaaacc caactcttcg tgaggtgttg cagtggctgg aggacaaagg
     4801 gcttagcgca tacagcatct cttgtggaag ctgtctactg ttcaacagta tgttcacaag
     4861 gcacaaggag aggatggaca agaaggttgt ggatctcgct cgggatgttg cgaaagtcga
     4921 gttaccgcct tatcgcaacc atcttgatgt agtggtggct tgtgaggatg aagatgacaa
     4981 tgacgtcgat attcctctcg tctctatcta cttcaggtga gcttactatt aactttaaga
     5041 aggagatata ccatgtaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc
     5101 ccggactatg caggattgta tccatatgac gttccagatt acgccactag agctgcttac
     5161 ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta tgcaggattg
     5221 tatccatatg acgttccaga ttacgcaatg gcttcgaaac ggatcttgaa ggagctgaag
     5281 gatctccaga aagaccctcc aacctcctgc agtgcaggtc cagttgctga ggacatgttt
     5341 cattggcaag ctacaattat gggtcctgca gagagtccgt attcaggcgg tgtgtttctc
     5401 gttaccattc acttccctcc ggactatcca ttcaaaccac caaaggttgc atttaggacg
     5461 aaggtgtttc accctaatat caacagcaac ggaagcattt gccttgacat tttgaaagaa
     5521 caatggagcc ctgccctcac catttccaag gtgttgctct cgatatgttc gctgttaaca
     5581 gatccaaatc cagatgaccc tttggtacca gagattgcac acatgtacaa aaccgacaga
     5641 gccaaatacg aggctactgc aagaaactgg actcagaagt atgccatggg ctaagctttt
     5701 acgcttttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5761 ctgctcccgg catccgctta cagacaagct gtgaccggga tgagatccgg ctgctaacaa
     5821 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     5881 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
     5941 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6001 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6061 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6121 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6181 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6241 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6301 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6361 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     6421 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6481 cgctcatgaa ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc
     6541 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     6601 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     6661 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     6721 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     6781 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     6841 ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt aaaaggacaa
     6901 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     6961 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     7021 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     7081 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     7141 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     7201 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     7261 ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc
     7321 cttgtattac tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg
     7381 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7441 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7501 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7561 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     7621 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7681 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7741 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7801 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7861 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7921 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7981 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8041 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8101 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8161 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     8221 ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc
     8281 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     8341 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     8401 cggcatccgc ttacagacaa gctgtgaccg actccgggag ctgcatgtgt cagaggtttt
     8461 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     8521 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     8581 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     8641 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     8701 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     8761 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     8821 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga
     8881 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa
     8941 cacggaaacc caagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
     9001 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc
     9061 agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc
     9121 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt
     9181 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc
     9241 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt
     9301 gcatgataaa gaacacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga
     9361 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag
     9421 tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     9481 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcg
//
