LOCUS       sequence-205563-       11595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation S375L with lower surface
            expression relative to wildtype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTS375L-myc
FEATURES             Location/Qualifiers
     source          1..11595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(785..803)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        871..975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(976..1044,1045..1836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1194..1213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2007..2595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2496..2515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3026..3171
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3214..4251
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4249..4268)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4293..4341
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4995..5374
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5375..5578
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5529..5549
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5575..5599
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5627..5636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6302..6331
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7620..7754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7644..7663)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7698..7717
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7897..7919)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8479..10268
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc
       61 cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt
      121 ggacgttttt ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc
      181 ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta
      241 tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc
      301 ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact
      361 aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact
      421 cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc
      481 caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag
      541 cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc
      601 aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc
      661 ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc
      721 ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
      781 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      841 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      901 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      961 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1021 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1081 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1141 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1201 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     1261 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1321 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1381 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1441 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1501 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1561 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1621 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1681 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     1741 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1801 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1861 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1921 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1981 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2041 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2101 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2161 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2221 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2281 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2341 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2401 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2461 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2521 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2581 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2641 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
     2701 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
     2761 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc
     2821 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag
     2881 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc
     2941 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
     3001 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
     3061 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
     3121 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
     3181 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
     3241 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     3301 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     3361 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     3421 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     3481 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     3541 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     3601 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     3661 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     3721 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     3781 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     3841 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     3901 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     3961 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     4021 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     4081 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     4141 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     4201 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
     4261 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     4321 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     4381 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     4441 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     4501 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
     4561 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
     4621 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
     4681 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
     4741 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     4801 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     4861 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     4921 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     4981 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     5041 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     5101 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     5161 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     5221 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     5281 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     5341 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     5401 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     5461 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     5521 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     5581 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatggagac
     5641 cacccccctg aacagtcaaa agcaactctc tgcctgtgag gatggagagg actgtcagga
     5701 gaatggcgtt cttcaaaagg ttgtacccac ccctggagat aaggtggaaa gcggccagat
     5761 ttccaacggc tacagcgccg tgccatcacc tggagccggg gatgatacac ggcacagcat
     5821 tcccgcaacc accacgacac tggttgcaga gcttcaccag ggcgagaggg agacgtgggg
     5881 caaaaaggta gattttctgc tttccgtgat cggttacgcg gtcgatctgg ggaacgtgtg
     5941 gaggttccct tacatttgtt accaaaacgg agggggtgcc ttcttgctgc cctacacaat
     6001 tatggcgatt tttgggggta tcccgctctt ctacatggaa ctggccttgg gtcagtacca
     6061 ccggaacggc tgcattagca tttggcgcaa gatatgcccc attttcaagg gaatcggata
     6121 cgcaatctgt ataatcgcgt tctatattgc atcatattac aacaccatca tggcctgggc
     6181 cctttattac ttgatcagca gtttcaccga ccaattgccg tggacgagct gtaagaactc
     6241 atggaatacc gggaactgta ccaactactt ctctgaggac agctcaggag gtagcggggg
     6301 tgaacagaag ctgattagcg aggaggactt gggcagtcca ggcggaagcg gcaacatcac
     6361 ttggacactc catagtacga gccccgcaga ggagttttac accaggcacg tactgcagat
     6421 tcataggagc aagggtctgc aagacctggg cggtattagc tggcagctcg cattgtgcat
     6481 aatgttgatc tttacggtga tctatttctc tatctggaag ggagtaaaga catccggcaa
     6541 ggtggtgtgg gtgacggcga ccttccccta cattatactc agcgtgttgt tggtgagggg
     6601 ggcaaccctg cctggggcct ggcgaggcgt gctgttctat ctcaagccaa actggcagaa
     6661 gcttcttgaa accggagtgt ggatcgacgc ggccgcgcaa atcttctttt ccctcggtcc
     6721 cggcttcggg gtccttctgg cattcgcaag ctacaacaag ttcaacaata actgctacca
     6781 ggacgccctc gtgacaagtg tagtgaactg catgacctct ttcgtgctcg ggtttgtgat
     6841 cttcacagta ctcggatata tggctgagat gaggaacgaa gatgttagtg aggttgccaa
     6901 agacgctggc ccgagcctgt tgttcatcac atacgccgag gctatcgcga acatgcccgc
     6961 atctactttc ttcgccatca tctttttcct gatgctgata acccttgggc tggatagcac
     7021 cttcgccgga ctggagggtg tcatcactgc cgttctcgac gagttccccc atgtgtgggc
     7081 caagcgacgg gaaaggttcg tgctggccgt cgtaatcaca tgcttcttcg gctcactggt
     7141 gacccttacc tttggcggtg cgtacgtggt caagctgctc gaagagtacg ccactggacc
     7201 ggcggttctg accgtggcat tgatcgaggc cgtggccgta agctggtttt acggcatcac
     7261 gcagttctgt agggacgtga aagagatgct gggcttttct cccggttggt tttggaggat
     7321 ttgttgggta gcaattagcc ccctgtttct ccttttcatt atttgctctt ttctgatgtc
     7381 tccaccgcag ctccgactgt ttcagtacaa ctacccctac tggtcaatta tcctgggcta
     7441 ttgtatcggc acctcaagtt tcatctgcat ccccacatac atcgcataca gactgataat
     7501 tacgcccggt acttttaagg agcgaatcat taaaagcata acccccgaaa cccccaccga
     7561 gatcccttgt ggcgatataa gactcaatgc ggtctaatag ctcgaggccg gcaaggccgg
     7621 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     7681 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     7741 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     7801 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     7861 cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     7921 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     7981 agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctagagg atcgatcccc
     8041 gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat
     8101 gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg
     8161 gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt
     8221 gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg
     8281 caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg
     8341 acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg
     8401 ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag
     8461 gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc
     8521 agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag
     8581 ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt
     8641 tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg
     8701 gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc
     8761 cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct
     8821 cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg
     8881 gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt
     8941 aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt
     9001 aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc
     9061 aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg
     9121 ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca
     9181 ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa
     9241 caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc
     9301 acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa
     9361 ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga
     9421 atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga
     9481 caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg
     9541 aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc
     9601 tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     9661 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     9721 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     9781 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt
     9841 agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga
     9901 tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     9961 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
    10021 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
    10081 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
    10141 tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga
    10201 gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc
    10261 ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg
    10321 catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga
    10381 gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg
    10441 aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg
    10501 gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa
    10561 caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc
    10621 atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa
    10681 atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt
    10741 tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa
    10801 aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc
    10861 aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag
    10921 ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc
    10981 ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct
    11041 taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc
    11101 aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg
    11161 gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc
    11221 accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa
    11281 aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact
    11341 actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg
    11401 acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc
    11461 ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc
    11521 tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
    11581 tgctcctgcc cctcc
//
