LOCUS       sequence-205565-        6354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses E. coli HPII catalase with TAG stop codon at site 392 and 
            N-terminal 6x his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Mehl R, van Zee K, Kean K
  TITLE     
  JOURNAL   2020
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "2020"
COMMENT     Sequence Label: pBad-HPII TAG392
FEATURES             Location/Qualifiers
     source          1..6354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..298
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     221..240
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             344..361
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             365..397
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(401..409,410..424)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(2768..2785)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2938..3024
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3116..3143
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3162..3253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3254..3322,3323..4114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3472..3491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4285..4873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4774..4793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5027..5044
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5059..5199
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5125..5144)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5285..5307
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5463..6341)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc
       61 ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa
      121 caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga
      181 aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc
      241 ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac
      301 ccgttttttg ggctaacagg aggaattaac catggggggt tctcatcatc atcatcatca
      361 tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga
      421 taaggatcga tggggatccg agctcgagtc gcaacataac gaaaagaacc cacatcagca
      481 ccagtcacca ctacacgatt ccagcgaagc gaaaccgggg atggactcac tggcacctga
      541 ggacggctct catcgtccag cggctgaacc aacaccgcca ggtgcacaac ctaccgcccc
      601 agggagcctg aaagcccctg atacgcgtaa cgaaaaactt aattctctgg aagacgtacg
      661 caaaggcagt gaaaattatg cgctgaccac taatcagggc gtgcgcatcg ccgacgatca
      721 aaactcactg cgtgccggta gccgtggtcc aacgctgctg gaagatttta ttctgcgcga
      781 gaaaatcacc cactttgacc atgagcgcat tccggaacgt attgttcatg cacgcggatc
      841 agccgctcac ggttatttcc agccatataa aagcttaagc gatattacca aagcggattt
      901 cctctcagat ccgaacaaaa tcaccccagt atttgtacgt ttctctaccg ttcagggtgg
      961 tgctggctct gctgataccg tgcgtgatat ccgtggcttt gccaccaagt tctataccga
     1021 agagggtatt tttgacctcg ttggcaataa cacgccaatc ttctttatcc aggatgcgca
     1081 taaattcccc gattttgttc atgcggtaaa accagaaccg cactgggcaa ttccacaagg
     1141 gcaaagtgcc cacgatactt tctgggatta tgtttctctg caacctgaaa ctctgcacaa
     1201 cgtgatgtgg gcgatgtcgg atcgcggcat cccccgcagt taccgcacca tggaaggctt
     1261 cggtattcac accttccgcc tgattaatgc cgaagggaag gcaacgtttg tacgtttcca
     1321 ctggaaacca ctggcaggta aagcctcact cgtttgggat gaagcacaaa aactcaccgg
     1381 acgtgacccg gacttccacc gccgcgagtt gtgggaagcc attgaagcag gcgattttcc
     1441 ggaatacgaa ctgggcttcc agttgattcc tgaagaagat gaattcaagt tcgacttcga
     1501 tcttctcgat ccaaccaaac ttatcccgga agaactggtg cccgttcagc gtgtcggcaa
     1561 aatggtgctc aatcgcaacc cggataactt ctttgctgaa aacgaacagg cggctttcta
     1621 gcctgggcat atcgtgccgg gactggactt caccaacgat ccgctgttgc agggacgttt
     1681 gttctcctat accgatacac aaatcagtcg tcttggtggg ccgaatttcc atgagattcc
     1741 gattaaccgt ccgacctgcc cttaccataa tttccagcgt gacggcatgc atcgcatggg
     1801 gatcgacact aacccggcga attacgaacc gaactcgatt aacgataact ggccgcgcga
     1861 aacaccgccg gggccgaaac gcggcggttt tgaatcatac caggagcgcg tggaaggcaa
     1921 taaagttcgc gagcgcagcc catcgtttgg cgaatattat tcccatccgc gtctgttctg
     1981 gctaagtcag acgccatttg agcagcgcca tattgtcgat ggtttcagtt ttgagttaag
     2041 caaagtcgtt cgtccgtata ttcgtgagcg cgttgttgac cagctggcgc atattgatct
     2101 cactctggcc caggcggtgg cgaaaaatct cggtatcgaa ctgactgacg accagctgaa
     2161 tatcacccca cctccggacg tcaacggtct gaaaaaggat ccatccttaa gtttgtacgc
     2221 cattcctgac ggtgatgtga aaggtcgcgt ggtagcgatt ttacttaatg atgaagtgag
     2281 atcggcagac cttctggcca ttctcaaggc gctgaaggcc aaaggcgttc atgccaaact
     2341 gctctactcc cgaatgggtg aagtgactgc ggatgacggt acggtgttgc ctatagccgc
     2401 tacctttgcc ggtgcacctt cgctgacggt cgatgcggtc attgtccctt gcggcaatat
     2461 cgcggatatc gctgacaacg gcgatgccaa ctactacctg atggaagcct acaaacacct
     2521 taaaccgatt gcgctggcgg gtgacgcgcg caagtttaaa gcaacaatca agatcgctga
     2581 ccagggtgaa gaagggattg tggaagctga cagcgctgac ggtagtttta tggatgaact
     2641 gctaacgctg atggcagcac accgcgtgtg gtcacgcatt cctaagattg acaaaattcc
     2701 tgcctgatgg ggtaccatat gggaattcga agcttggctg ttttggcgga tgagagaaga
     2761 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
     2821 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
     2881 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
     2941 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     3001 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
     3061 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
     3121 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
     3181 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3241 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3301 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3361 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3421 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3481 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     3541 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3601 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3661 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3721 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3781 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3841 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3901 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3961 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4021 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4081 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4141 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4201 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4261 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4321 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4381 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4441 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4501 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4561 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4621 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4681 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4741 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4801 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4861 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4921 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4981 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5041 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5101 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     5161 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     5221 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5281 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     5341 cagatcaatt cgcgcgcgaa ggcgaagcgg catgcataat gtgcctgtca aatggacgaa
     5401 gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc
     5461 aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg
     5521 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa
     5581 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc
     5641 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa
     5701 agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa
     5761 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc
     5821 ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga
     5881 tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca
     5941 aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca
     6001 aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac
     6061 tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg
     6121 aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc
     6181 tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa
     6241 aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac
     6301 gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat actc
//
