LOCUS       sequence-205572-       11489 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation I425V conferring 
            aproximately wildtype phenotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTI425V-myc
FEATURES             Location/Qualifiers
     source          1..11489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(702..720)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        788..892
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(893..961,962..1753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1111..1130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1924..2512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2413..2432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2943..3088
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3131..4168
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4166..4185)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4210..4258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4912..5291
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5292..5495
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5446..5466
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5492..5516
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5544..5553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6219..6248
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7537..7671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7561..7580)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7615..7634
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7814..7836)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8396..10185
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac
       61 accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc
      121 tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc
      181 actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg
      241 tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt
      301 attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc
      361 caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct
      421 ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat
      481 cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc
      541 tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca
      601 gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg
      661 atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt
      721 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      781 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      841 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      901 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      961 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1021 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1081 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     1141 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1201 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1261 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1321 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1381 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     1441 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1501 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1561 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     1621 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1681 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1741 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1801 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1861 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1921 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1981 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2041 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     2101 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2161 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2221 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2281 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2341 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2401 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2461 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2521 caacgcggcc tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc
     2581 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
     2641 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta
     2701 gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
     2761 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
     2821 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc
     2881 gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta
     2941 tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt
     3001 cggggcggcg cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca
     3061 ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg
     3121 gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     3181 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     3241 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     3301 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     3361 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     3421 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     3481 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     3541 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     3601 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     3661 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     3721 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     3781 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     3841 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     3901 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     3961 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     4021 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     4081 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     4141 ggatcgggag atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     4201 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
     4261 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     4321 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     4381 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagccactgg ccccgtgggt
     4441 tagggacggg gtcccccatg gggaatggtt tatggttcgt gggggttatt attttgggcg
     4501 ttgcgtgggg tcaggtccac gactggactg agcagacaga cccatggttt ttggatggcc
     4561 tgggcatgga ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca
     4621 cccccgaccc ccaaaaacca ccgcgcggat ttctggcgtg ccaagctagt cgaccaattc
     4681 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     4741 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
     4801 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     4861 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat
     4921 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4981 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     5041 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     5101 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     5161 atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     5221 cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg
     5281 ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact
     5341 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     5401 atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta
     5461 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatctcta
     5521 gaagctgggt accagctgct agcgccacca tggagaccac ccccctgaac agtcaaaagc
     5581 aactctctgc ctgtgaggat ggagaggact gtcaggagaa tggcgttctt caaaaggttg
     5641 tacccacccc tggagataag gtggaaagcg gccagatttc caacggctac agcgccgtgc
     5701 catcacctgg agccggggat gatacacggc acagcattcc cgcaaccacc acgacactgg
     5761 ttgcagagct tcaccagggc gagagggaga cgtggggcaa aaaggtagat tttctgcttt
     5821 ccgtgatcgg ttacgcggtc gatctgggga acgtgtggag gttcccttac atttgttacc
     5881 aaaacggagg gggtgccttc ttgctgccct acacaattat ggcgattttt gggggtatcc
     5941 cgctcttcta catggaactg gccttgggtc agtaccaccg gaacggctgc attagcattt
     6001 ggcgcaagat atgccccatt ttcaagggaa tcggatacgc aatctgtata atcgcgttct
     6061 atattgcatc atattacaac accatcatgg cctgggccct ttattacttg atcagcagtt
     6121 tcaccgacca attgccgtgg acgagctgta agaactcatg gaataccggg aactgtacca
     6181 actacttctc tgaggacagc tcaggaggta gcgggggtga acagaagctg attagcgagg
     6241 aggacttggg cagtccaggc ggaagcggca acatcacttg gacactccat agtacgagcc
     6301 ccgcagagga gttttacacc aggcacgtac tgcagattca taggagcaag ggtctgcaag
     6361 acctgggcgg tattagctgg cagctcgcat tgtgcataat gttgatcttt acggtgatct
     6421 atttctctat ctggaaggga gtaaagacat ccggcaaggt ggtgtgggtg acggcgacct
     6481 tcccctacat tatactcagc gtgttgttgg tgaggggggc aaccctgcct ggggcctggc
     6541 gaggcgtgct gttctatctc aagccaaact ggcagaagct tcttgaaacc ggagtgtgga
     6601 tcgacgcggc cgcgcaaatc ttcttttccc tcggtcccgg cttcggggtc cttctggcat
     6661 tcgcaagcta caacaagttc aacaataact gctaccagga cgccctcgtg acaagtgtag
     6721 tgaactgcat gacctctttc gtgagcgggt ttgtgatctt cacagtactc ggatatatgg
     6781 ctgagatgag gaacgaagat gttagtgagg ttgccaaaga cgctggcccg agcctgttgt
     6841 tcatcacata cgccgaggct atcgcgaaca tgcccgcatc tactttcttc gccgtcatct
     6901 ttttcctgat gctgataacc cttgggctgg atagcacctt cgccggactg gagggtgtca
     6961 tcactgccgt tctcgacgag ttcccccatg tgtgggccaa gcgacgggaa aggttcgtgc
     7021 tggccgtcgt aatcacatgc ttcttcggct cactggtgac ccttaccttt ggcggtgcgt
     7081 acgtggtcaa gctgctcgaa gagtacgcca ctggaccggc ggttctgacc gtggcattga
     7141 tcgaggccgt ggccgtaagc tggttttacg gcatcacgca gttctgtagg gacgtgaaag
     7201 agatgctggg cttttctccc ggttggtttt ggaggatttg ttgggtagca attagccccc
     7261 tgtttctcct tttcattatt tgctcttttc tgatgtctcc accgcagctc cgactgtttc
     7321 agtacaacta cccctactgg tcaattatcc tgggctattg tatcggcacc tcaagtttca
     7381 tctgcatccc cacatacatc gcatacagac tgataattac gcccggtact tttaaggagc
     7441 gaatcattaa aagcataacc cccgaaaccc ccaccgagat cccttgtggc gatataagac
     7501 tcaatgcggt ctaatagctc gaggccggca aggccggatc cagacatgat aagatacatt
     7561 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     7621 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     7681 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     7741 taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg
     7801 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     7861 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     7921 gcgcagccat gaggtcgact ctagaggatc gatccccgcc ccggacgaac taaacctgac
     7981 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     8041 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     8101 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     8161 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     8221 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     8281 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     8341 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     8401 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     8461 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     8521 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     8581 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     8641 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     8701 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     8761 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     8821 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     8881 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     8941 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     9001 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     9061 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     9121 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     9181 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     9241 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     9301 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     9361 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     9421 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     9481 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     9541 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     9601 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     9661 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     9721 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     9781 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     9841 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     9901 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     9961 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
    10021 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat
    10081 atacagtcag catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc
    10141 cacctcccaa gggggcgtga attttcgctg cttgtccttt tcctgctggt tgctcccatt
    10201 cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta
    10261 aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca
    10321 acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc
    10381 cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga
    10441 atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt
    10501 cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg
    10561 cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc
    10621 acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg
    10681 catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa
    10741 tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac
    10801 gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt
    10861 aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg
    10921 tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa
    10981 atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc
    11041 tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg
    11101 ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt
    11161 ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg
    11221 gcctgcgcgg tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt
    11281 ctccacgtcc acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac
    11341 gtcctctacc ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac
    11401 cccggcctcc actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc
    11461 ctgcccctcc tcctgctcct gcccctcct
//
