LOCUS       sequence-205575-       11533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation I425L conferring 
            approximately wildtype transport phenotype, decreased surface 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTI425L-myc
FEATURES             Location/Qualifiers
     source          1..11533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(735..753)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        821..925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(926..994,995..1786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1144..1163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1957..2545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2446..2465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2976..3121
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3164..4201
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4199..4218)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4243..4291
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4945..5324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5325..5528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5479..5499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5525..5549
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5577..5586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6252..6281
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7570..7704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7594..7613)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7648..7667
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7847..7869)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8429..10218
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc
       61 aatgcaactt ggacgttttt ggggtctccg gacaccatct ctatgtcttg gccctgatcc
      121 tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg
      181 tccatggtta tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga
      241 tgtgtctccc ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat
      301 gattcacact aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg
      361 agtgcagact cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt
      421 cagacagatc caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa
      481 ttactcgcag cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa
      541 gccaggcctc aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg
      601 acccctcctc ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa
      661 agctgggtgc ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc
      721 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      781 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      841 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      901 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      961 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1021 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1081 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1141 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     1201 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1261 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1321 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1381 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1441 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     1501 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1561 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1621 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1681 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1741 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1801 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1861 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1921 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1981 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2041 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2101 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2161 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2221 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2281 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2341 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2401 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2461 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2521 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2581 cttttgctgg ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc
     2641 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat
     2701 ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc
     2761 cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc
     2821 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
     2881 gcgaggtgcc gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc
     2941 cggaagaaat atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct
     3001 tgtcattggc gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt
     3061 cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta
     3121 acagcgtcaa cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact
     3181 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     3241 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     3301 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     3361 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     3421 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     3481 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     3541 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     3601 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     3661 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     3721 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     3781 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     3841 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     3901 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     3961 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     4021 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     4081 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     4141 cgatagtgga aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg
     4201 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     4261 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     4321 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     4381 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     4441 gcaggccctg ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg
     4501 gtttatggtt cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga
     4561 ctgagcagac agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg
     4621 acacgaacac cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg
     4681 gatttctggc gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     4741 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     4801 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     4861 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     4921 catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
     4981 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     5041 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     5101 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     5161 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
     5221 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     5281 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     5341 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     5401 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     5461 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     5521 gcagagctcg tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca
     5581 ccatggagac cacccccctg aacagtcaaa agcaactctc tgcctgtgag gatggagagg
     5641 actgtcagga gaatggcgtt cttcaaaagg ttgtacccac ccctggagat aaggtggaaa
     5701 gcggccagat ttccaacggc tacagcgccg tgccatcacc tggagccggg gatgatacac
     5761 ggcacagcat tcccgcaacc accacgacac tggttgcaga gcttcaccag ggcgagaggg
     5821 agacgtgggg caaaaaggta gattttctgc tttccgtgat cggttacgcg gtcgatctgg
     5881 ggaacgtgtg gaggttccct tacatttgtt accaaaacgg agggggtgcc ttcttgctgc
     5941 cctacacaat tatggcgatt tttgggggta tcccgctctt ctacatggaa ctggccttgg
     6001 gtcagtacca ccggaacggc tgcattagca tttggcgcaa gatatgcccc attttcaagg
     6061 gaatcggata cgcaatctgt ataatcgcgt tctatattgc atcatattac aacaccatca
     6121 tggcctgggc cctttattac ttgatcagca gtttcaccga ccaattgccg tggacgagct
     6181 gtaagaactc atggaatacc gggaactgta ccaactactt ctctgaggac agctcaggag
     6241 gtagcggggg tgaacagaag ctgattagcg aggaggactt gggcagtcca ggcggaagcg
     6301 gcaacatcac ttggacactc catagtacga gccccgcaga ggagttttac accaggcacg
     6361 tactgcagat tcataggagc aagggtctgc aagacctggg cggtattagc tggcagctcg
     6421 cattgtgcat aatgttgatc tttacggtga tctatttctc tatctggaag ggagtaaaga
     6481 catccggcaa ggtggtgtgg gtgacggcga ccttccccta cattatactc agcgtgttgt
     6541 tggtgagggg ggcaaccctg cctggggcct ggcgaggcgt gctgttctat ctcaagccaa
     6601 actggcagaa gcttcttgaa accggagtgt ggatcgacgc ggccgcgcaa atcttctttt
     6661 ccctcggtcc cggcttcggg gtccttctgg cattcgcaag ctacaacaag ttcaacaata
     6721 actgctacca ggacgccctc gtgacaagtg tagtgaactg catgacctct ttcgtgagcg
     6781 ggtttgtgat cttcacagta ctcggatata tggctgagat gaggaacgaa gatgttagtg
     6841 aggttgccaa agacgctggc ccgagcctgt tgttcatcac atacgccgag gctatcgcga
     6901 acatgcccgc atctactttc ttcgccctca tctttttcct gatgctgata acccttgggc
     6961 tggatagcac cttcgccgga ctggagggtg tcatcactgc cgttctcgac gagttccccc
     7021 atgtgtgggc caagcgacgg gaaaggttcg tgctggccgt cgtaatcaca tgcttcttcg
     7081 gctcactggt gacccttacc tttggcggtg cgtacgtggt caagctgctc gaagagtacg
     7141 ccactggacc ggcggttctg accgtggcat tgatcgaggc cgtggccgta agctggtttt
     7201 acggcatcac gcagttctgt agggacgtga aagagatgct gggcttttct cccggttggt
     7261 tttggaggat ttgttgggta gcaattagcc ccctgtttct ccttttcatt atttgctctt
     7321 ttctgatgtc tccaccgcag ctccgactgt ttcagtacaa ctacccctac tggtcaatta
     7381 tcctgggcta ttgtatcggc acctcaagtt tcatctgcat ccccacatac atcgcataca
     7441 gactgataat tacgcccggt acttttaagg agcgaatcat taaaagcata acccccgaaa
     7501 cccccaccga gatcccttgt ggcgatataa gactcaatgc ggtctaatag ctcgaggccg
     7561 gcaaggccgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     7621 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     7681 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     7741 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     7801 gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     7861 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     7921 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctagagg
     7981 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     8041 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     8101 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     8161 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     8221 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     8281 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     8341 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     8401 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     8461 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     8521 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     8581 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     8641 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     8701 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     8761 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     8821 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     8881 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     8941 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     9001 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     9061 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     9121 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     9181 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     9241 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     9301 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     9361 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     9421 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     9481 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     9541 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     9601 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     9661 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     9721 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     9781 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     9841 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     9901 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     9961 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
    10021 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
    10081 tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag
    10141 tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg
    10201 ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct
    10261 aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag
    10321 aaggtgttga gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt
    10381 tgggaggacg aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat
    10441 gtctactggg gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg
    10501 cgtctcctaa caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt
    10561 catctccgtc atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc
    10621 agggaggcaa atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag
    10681 gagcgggctt tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat
    10741 gagtttgtaa aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg
    10801 ggccgggtcc aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg
    10861 gaatagcaag ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata
    10921 cgaacacacc ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca
    10981 ggagagctct taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac
    11041 gatgctttcc aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc
    11101 cagtgcttgg gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca
    11161 cgtcaggctc accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta
    11221 cagggtggaa aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg
    11281 atgactgact actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg
    11341 ctctttcacg acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac
    11401 ctcctcgacc ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc
    11461 ggcctccacc tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc
    11521 ctgcccctcc tgc
//
