LOCUS       sequence-205581-       11521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation T439M, decreased surface
            expression and transport relative to wildtype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTT439M-myc
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(732..750)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        818..922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(923..991,992..1783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1141..1160)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1954..2542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2443..2462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2973..3118
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3161..4198
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4196..4215)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4240..4288
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4942..5321
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5322..5525
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5476..5496
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5522..5546
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5574..5583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6249..6278
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7567..7701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7591..7610)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7645..7664
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7844..7866)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8426..10215
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcctgcccct cccgctcctg ctcctgctcc tgttccaccg tgggtccctt tgcagccaat
       61 gcaacttgga cgtttttggg gtctccggac accatctcta tgtcttggcc ctgatcctga
      121 gccgcccggg gctcctggtc ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc
      181 atggttatca ccccctcttc tttgaggtcc actgccgccg gagccttctg gtccagatgt
      241 gtctcccttc tctcctaggc catttccagg tcctgtacct ggcccctcgt cagacatgat
      301 tcacactaaa agagatcaat agacatcttt attagacgac gctcagtgaa tacagggagt
      361 gcagactcct gccccctcca acagcccccc caccctcatc cccttcatgg tcgctgtcag
      421 acagatccag gtctgaaaat tccccatcct ccgaaccatc ctcgtcctca tcaccaatta
      481 ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc
      541 aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag ggatggggat tctcgggacc
      601 cctcctcttc ctcttcaagg tcaccagaca gagatgctac tggggcaacg gaagaaaagc
      661 tgggtgcggc ctgtgaggat cagcttatcg atgataagct gtcaaacatg agaattcttg
      721 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      781 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      841 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      901 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      961 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1021 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1081 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1141 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     1201 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1261 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1321 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1381 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1441 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     1501 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1561 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1621 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1681 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1741 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1801 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1861 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1921 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1981 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2041 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2101 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2161 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2221 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2281 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2341 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2401 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2461 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2521 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2581 ttgctggcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     2641 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     2701 gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccccga gatgcgccgc
     2761 gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca
     2821 ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg
     2881 aggtgccgcc ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg
     2941 aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt
     3001 cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtccgagg tccacttcgc
     3061 atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca
     3121 gcgtcaacag cgtgccgcag atcccggggg gcaatgagat atgaaaaagc ctgaactcac
     3181 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     3241 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     3301 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     3361 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     3421 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     3481 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     3541 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     3601 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     3661 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     3721 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     3781 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     3841 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     3901 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     3961 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     4021 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     4081 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     4141 tagtggaaac cgacgcccca gcactcgtcc ggatcgggag atgggggagg ctaactgaaa
     4201 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     4261 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     4321 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     4381 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     4441 ggccctgcca tagccactgg ccccgtgggt tagggacggg gtcccccatg gggaatggtt
     4501 tatggttcgt gggggttatt attttgggcg ttgcgtgggg tcaggtccac gactggactg
     4561 agcagacaga cccatggttt ttggatggcc tgggcatgga ccgcatgtac tggcgcgaca
     4621 cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc ccaaaaacca ccgcgcggat
     4681 ttctggcgtg ccaagctagt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     4741 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat
     4801 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     4861 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     4921 gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
     4981 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     5041 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     5101 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     5161 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     5221 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     5281 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     5341 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     5401 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     5461 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     5521 gagctcgttt agtgaaccgt cagatctcta gaagctgggt accagctgct agcgccacca
     5581 tggagaccac ccccctgaac agtcaaaagc aactctctgc ctgtgaggat ggagaggact
     5641 gtcaggagaa tggcgttctt caaaaggttg tacccacccc tggagataag gtggaaagcg
     5701 gccagatttc caacggctac agcgccgtgc catcacctgg agccggggat gatacacggc
     5761 acagcattcc cgcaaccacc acgacactgg ttgcagagct tcaccagggc gagagggaga
     5821 cgtggggcaa aaaggtagat tttctgcttt ccgtgatcgg ttacgcggtc gatctgggga
     5881 acgtgtggag gttcccttac atttgttacc aaaacggagg gggtgccttc ttgctgccct
     5941 acacaattat ggcgattttt gggggtatcc cgctcttcta catggaactg gccttgggtc
     6001 agtaccaccg gaacggctgc attagcattt ggcgcaagat atgccccatt ttcaagggaa
     6061 tcggatacgc aatctgtata atcgcgttct atattgcatc atattacaac accatcatgg
     6121 cctgggccct ttattacttg atcagcagtt tcaccgacca attgccgtgg acgagctgta
     6181 agaactcatg gaataccggg aactgtacca actacttctc tgaggacagc tcaggaggta
     6241 gcgggggtga acagaagctg attagcgagg aggacttggg cagtccaggc ggaagcggca
     6301 acatcacttg gacactccat agtacgagcc ccgcagagga gttttacacc aggcacgtac
     6361 tgcagattca taggagcaag ggtctgcaag acctgggcgg tattagctgg cagctcgcat
     6421 tgtgcataat gttgatcttt acggtgatct atttctctat ctggaaggga gtaaagacat
     6481 ccggcaaggt ggtgtgggtg acggcgacct tcccctacat tatactcagc gtgttgttgg
     6541 tgaggggggc aaccctgcct ggggcctggc gaggcgtgct gttctatctc aagccaaact
     6601 ggcagaagct tcttgaaacc ggagtgtgga tcgacgcggc cgcgcaaatc ttcttttccc
     6661 tcggtcccgg cttcggggtc cttctggcat tcgcaagcta caacaagttc aacaataact
     6721 gctaccagga cgccctcgtg acaagtgtag tgaactgcat gacctctttc gtgagcgggt
     6781 ttgtgatctt cacagtactc ggatatatgg ctgagatgag gaacgaagat gttagtgagg
     6841 ttgccaaaga cgctggcccg agcctgttgt tcatcacata cgccgaggct atcgcgaaca
     6901 tgcccgcatc tactttcttc gccatcatct ttttcctgat gctgataacc cttgggctgg
     6961 atagcatgtt cgccggactg gagggtgtca tcactgccgt tctcgacgag ttcccccatg
     7021 tgtgggccaa gcgacgggaa aggttcgtgc tggccgtcgt aatcacatgc ttcttcggct
     7081 cactggtgac ccttaccttt ggcggtgcgt acgtggtcaa gctgctcgaa gagtacgcca
     7141 ctggaccggc ggttctgacc gtggcattga tcgaggccgt ggccgtaagc tggttttacg
     7201 gcatcacgca gttctgtagg gacgtgaaag agatgctggg cttttctccc ggttggtttt
     7261 ggaggatttg ttgggtagca attagccccc tgtttctcct tttcattatt tgctcttttc
     7321 tgatgtctcc accgcagctc cgactgtttc agtacaacta cccctactgg tcaattatcc
     7381 tgggctattg tatcggcacc tcaagtttca tctgcatccc cacatacatc gcatacagac
     7441 tgataattac gcccggtact tttaaggagc gaatcattaa aagcataacc cccgaaaccc
     7501 ccaccgagat cccttgtggc gatataagac tcaatgcggt ctaatagctc gaggccggca
     7561 aggccggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     7621 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     7681 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     7741 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     7801 tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     7861 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     7921 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaggtcgact ctagaggatc
     7981 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     8041 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     8101 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     8161 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     8221 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     8281 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     8341 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     8401 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     8461 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     8521 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     8581 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     8641 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     8701 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     8761 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     8821 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     8881 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     8941 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     9001 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     9061 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     9121 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     9181 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     9241 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     9301 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     9361 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     9421 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     9481 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     9541 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     9601 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     9661 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     9721 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     9781 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     9841 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     9901 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     9961 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
    10021 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
    10081 atccgggtag catatgctat cctcatgcat atacagtcag catatgatac ccagtagtag
    10141 agtgggagtg ctatcctttg catatgccgc cacctcccaa gggggcgtga attttcgctg
    10201 cttgtccttt tcctgctggt tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa
    10261 gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta atgttttcca acgcgagaag
    10321 gtgttgagcg cggagctgag tgacgtgaca acatgggtat gcccaattgc cccatgttgg
    10381 gaggacgaaa atggtgacaa gacagatggc cagaaataca ccaacagcac gcatgatgtc
    10441 tactggggat ttattcttta gtgcggggga atacacggct tttaatacga ttgagggcgt
    10501 ctcctaacaa gttacatcac tcctgccctt cctcaccctc atctccatca cctccttcat
    10561 ctccgtcatc tccgtcatca ccctccgcgg cagccccttc caccataggt ggaaaccagg
    10621 gaggcaaatc tactccatcg tcaaagctgc acacagtcac cctgatattg caggtaggag
    10681 cgggctttgt cataacaagg tccttaatcg catccttcaa aacctcagca aatatatgag
    10741 tttgtaaaaa gaccatgaaa taacagacaa tggactccct tagcgggcca ggttgtgggc
    10801 cgggtccagg ggccattcca aaggggagac gactcaatgg tgtaagacga cattgtggaa
    10861 tagcaagggc agttcctcgc cttaggttgt aaagggaggt cttactacct ccatatacga
    10921 acacaccggc gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc ctagccagga
    10981 gagctcttaa accttctgca atgttctcaa atttcgggtt ggaacctcct tgaccacgat
    11041 gctttccaaa ccaccctcct tttttgcgcc tgcctccatc accctgaccc cggggtccag
    11101 tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc gggggcacgt
    11161 caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct tcccctacag
    11221 ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg gatgatgatg
    11281 actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc cccctggctc
    11341 tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct ccactacctc
    11401 ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct cgaccccggc
    11461 ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
    11521 c
//
