LOCUS       sequence-205584-       11521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation T448K conferring gain of
            function phenotype, decreased surface expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTT448K-myc
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(708..726)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        794..898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(899..967,968..1759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1117..1136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1930..2518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2419..2438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2949..3094
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3137..4174
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4172..4191)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4216..4264
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4918..5297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5298..5501
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5452..5472
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5498..5522
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5550..5559
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6225..6254
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7543..7677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7567..7586)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7621..7640
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7820..7842)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8402..10191
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct
       61 ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc
      121 gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg
      181 aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt
      241 tccaggtcct gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac
      301 atctttatta gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag
      361 cccccccacc ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc
      421 catcctccga accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct
      481 gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct
      541 ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac
      601 cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc
      661 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg
      721 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      781 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      841 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      901 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      961 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1021 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1081 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1141 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1201 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1261 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1321 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1381 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1441 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1501 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1561 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1621 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1681 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1741 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1801 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1861 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1921 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1981 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2041 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2101 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2161 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2221 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2281 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2341 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2401 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2461 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2521 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg
     2581 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     2641 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     2701 gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac
     2761 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
     2821 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg
     2881 tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta
     2941 gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga
     3001 tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct
     3061 cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc
     3121 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     3181 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     3241 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     3301 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     3361 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     3421 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     3481 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     3541 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     3601 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     3661 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     3721 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     3781 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     3841 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     3901 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     3961 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     4021 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     4081 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     4141 tcgtccggat cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     4201 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     4261 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     4321 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     4381 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc
     4441 gtgggttagg gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt
     4501 tgggcgttgc gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg
     4561 atggcctggg catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc
     4621 caaacacccc cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac
     4681 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     4741 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     4801 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     4861 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
     4921 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     4981 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     5041 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     5101 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     5161 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     5221 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
     5281 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
     5341 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     5401 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
     5461 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     5521 tctctagaag ctgggtacca gctgctagcg ccaccatgga gaccaccccc ctgaacagtc
     5581 aaaagcaact ctctgcctgt gaggatggag aggactgtca ggagaatggc gttcttcaaa
     5641 aggttgtacc cacccctgga gataaggtgg aaagcggcca gatttccaac ggctacagcg
     5701 ccgtgccatc acctggagcc ggggatgata cacggcacag cattcccgca accaccacga
     5761 cactggttgc agagcttcac cagggcgaga gggagacgtg gggcaaaaag gtagattttc
     5821 tgctttccgt gatcggttac gcggtcgatc tggggaacgt gtggaggttc ccttacattt
     5881 gttaccaaaa cggagggggt gccttcttgc tgccctacac aattatggcg atttttgggg
     5941 gtatcccgct cttctacatg gaactggcct tgggtcagta ccaccggaac ggctgcatta
     6001 gcatttggcg caagatatgc cccattttca agggaatcgg atacgcaatc tgtataatcg
     6061 cgttctatat tgcatcatat tacaacacca tcatggcctg ggccctttat tacttgatca
     6121 gcagtttcac cgaccaattg ccgtggacga gctgtaagaa ctcatggaat accgggaact
     6181 gtaccaacta cttctctgag gacagctcag gaggtagcgg gggtgaacag aagctgatta
     6241 gcgaggagga cttgggcagt ccaggcggaa gcggcaacat cacttggaca ctccatagta
     6301 cgagccccgc agaggagttt tacaccaggc acgtactgca gattcatagg agcaagggtc
     6361 tgcaagacct gggcggtatt agctggcagc tcgcattgtg cataatgttg atctttacgg
     6421 tgatctattt ctctatctgg aagggagtaa agacatccgg caaggtggtg tgggtgacgg
     6481 cgaccttccc ctacattata ctcagcgtgt tgttggtgag gggggcaacc ctgcctgggg
     6541 cctggcgagg cgtgctgttc tatctcaagc caaactggca gaagcttctt gaaaccggag
     6601 tgtggatcga cgcggccgcg caaatcttct tttccctcgg tcccggcttc ggggtccttc
     6661 tggcattcgc aagctacaac aagttcaaca ataactgcta ccaggacgcc ctcgtgacaa
     6721 gtgtagtgaa ctgcatgacc tctttcgtga gcgggtttgt gatcttcaca gtactcggat
     6781 atatggctga gatgaggaac gaagatgtta gtgaggttgc caaagacgct ggcccgagcc
     6841 tgttgttcat cacatacgcc gaggctatcg cgaacatgcc cgcatctact ttcttcgcca
     6901 tcatcttttt cctgatgctg ataacccttg ggctggatag caccttcgcc ggactggagg
     6961 gtgtcatcaa ggccgttctc gacgagttcc cccatgtgtg ggccaagcga cgggaaaggt
     7021 tcgtgctggc cgtcgtaatc acatgcttct tcggctcact ggtgaccctt acctttggcg
     7081 gtgcgtacgt ggtcaagctg ctcgaagagt acgccactgg accggcggtt ctgaccgtgg
     7141 cattgatcga ggccgtggcc gtaagctggt tttacggcat cacgcagttc tgtagggacg
     7201 tgaaagagat gctgggcttt tctcccggtt ggttttggag gatttgttgg gtagcaatta
     7261 gccccctgtt tctccttttc attatttgct cttttctgat gtctccaccg cagctccgac
     7321 tgtttcagta caactacccc tactggtcaa ttatcctggg ctattgtatc ggcacctcaa
     7381 gtttcatctg catccccaca tacatcgcat acagactgat aattacgccc ggtactttta
     7441 aggagcgaat cattaaaagc ataacccccg aaacccccac cgagatccct tgtggcgata
     7501 taagactcaa tgcggtctaa tagctcgagg ccggcaaggc cggatccaga catgataaga
     7561 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     7621 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     7681 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     7741 agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt
     7801 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7861 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7921 gtcggggcgc agccatgagg tcgactctag aggatcgatc cccgccccgg acgaactaaa
     7981 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     8041 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     8101 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     8161 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     8221 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     8281 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     8341 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     8401 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     8461 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     8521 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     8581 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     8641 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     8701 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     8761 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     8821 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     8881 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     8941 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     9001 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     9061 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     9121 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     9181 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     9241 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     9301 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     9361 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     9421 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     9481 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     9541 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     9601 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     9661 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     9721 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     9781 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     9841 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     9901 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     9961 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
    10021 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
    10081 atgcatatac agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata
    10141 tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gctggttgct
    10201 cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac
    10261 caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac
    10321 gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca
    10381 gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc
    10441 gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta catcactcct
    10501 gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct
    10561 ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa
    10621 agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct
    10681 taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac
    10741 agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg
    10801 ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta
    10861 ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt
    10921 cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt
    10981 tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt
    11041 tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat
    11101 ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt
    11161 cttggtggta ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg
    11221 gagggggcct gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc
    11281 tcttttctcc acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc
    11341 tttcacgtcc tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc
    11401 ctcgaccccg gcctccactg cctcctcgac cccggcctcc acctcctgct cctgcccctc
    11461 ctgctcctgc ccctcctcct gctcctgccc ctcctgctcc tgcccctccc gctcctgctc
    11521 c
//
