LOCUS       sequence-205595-        2103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pAGT861
FEATURES             Location/Qualifiers
     source          1..2103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             234..1025
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1366..1954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1855..1874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2014..2031
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
       61 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
      121 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
      181 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
      241 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
      301 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
      361 agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
      421 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
      481 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
      541 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
      601 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
      661 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
      721 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
      781 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
      841 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
      901 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
      961 cagatcagtt ggaagaattt gtccattacg tgaaaggcga gatcaccaag gtagtcggca
     1021 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     1081 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     1141 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgctgtc
     1201 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1261 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1321 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1381 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1441 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1501 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1561 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1621 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1681 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1741 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1801 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1861 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1921 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1981 gttcctgcac tctgtggtct caccatgggc agccaccacc accaccacca cagcggaatg
     2041 tgagaccacg aagtgccgag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     2101 tgt
//
