LOCUS       sequence-20560-e         519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GPD-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(35..159)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(167..183)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(167..183)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(189..452)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(272..295)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aagtgtaaag cctgggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt
       61 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
      121 attttacgtt tctcgttcag cttttttgta caaacttgtg atgggggatc cactagttct
      181 agaatccgtc gaaactaagt tctggtgttt taaaactaaa aaaaagacta actataaaag
      241 tagaatttaa gaagtttaag aaatagattt acagaattac aatcaatacc taccgtcttt
      301 atatacttat tagtcaagta ggggaataat ttcagggaac tggtttcaac cttttttttc
      361 agctttttcc aaatcagaga gagcagaagg taatagaagg tgtaagaaaa tgagatagat
      421 acatgcgtgg gtcaattgcc ttgtgtcatc atttactcca ggcaggttgc atcactccat
      481 tgaggttgtg cccgtttttt gcctgtttgt gcccctgtt
//
