LOCUS       sequence-205604-        1929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondria targetting gTEMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Nakano M, Arai Y, Kotera I, Okabe K, Kamei Y, Nagai T
  TITLE     Genetically encoded ratiometric fluorescent thermometer with wide 
            range and rapid response.
  JOURNAL   PLoS One. 2017 Feb 17;12(2):e0172344. doi: 
            10.1371/journal.pone.0172344. eCollection 2017.
  PUBMED    28212432
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-02-17"; volume: "12(2):e0172344. doi"; pages: " 
            10.1371/journal.pone.0172344. eCollection 2017"
COMMENT     Sequence Label: mito-gTEMP_pcDNA3
FEATURES             Location/Qualifiers
     source          1..1929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             97..171
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             673..924
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKAHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLESVTAAGITLGMDELYK"
     CDS             943..996
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1003..1077
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1099..1173
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(1210..1212,1213..1215,1216..1926)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 atgtctgttc tgactcctct gctgctccgg ggtctcacag gttccgcaag aagactcccc
       61 gtgcctaggg ccaaaattca ttcactgggg gaccccatga gcgtgctcac cccactcctg
      121 ctgcgggggc tgaccggcag cgctaggcgg ctgccagtcc ccagggccaa gatccacagt
      181 ctcggcgatc ccaagcgcgg ccgcatcatg gtgagcaagg gcgaggagct gttcaccggg
      241 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc
      301 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctcat ctgcaccacc
      361 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgcagttcgg cgtgctgtgc
      421 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      481 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      541 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      601 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acgggataag ctcaaacgta
      661 tatatcaccg ccgacaagca gaagaacggc atcaaggccc acttcaagat ccgccacaac
      721 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      781 ggccccgtgc tgctgcccga caaccactac ctgagcgtcc agtccaagct gagcaaagac
      841 cccaacgaga agcgcgatca catggtcctg ctggagtccg tgaccgccgc cgggatcact
      901 ctcggcatgg acgagctgta caagcgcatg catggcagcg gcgagggcag aggcagcctg
      961 ctgacctgcg gcgacgtgga ggagaacccc ggccccgagc tcatgtctgt tctgactcct
     1021 ctgctgctcc ggggtctcac aggttccgca agaagactcc ccgtgcctag ggccaaaatt
     1081 cattcactgg gggaccccat gagcgtgctc accccactcc tgctgcgggg gctgaccggc
     1141 agcgctaggc ggctgccagt ccccagggcc aagatccaca gtctcggcga tcccaagcgc
     1201 ggccgcatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1261 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1321 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1381 tggcccaccc tcgtgaccac cttctcctac ggcgtgatgg tgttcgcccg ctaccccgac
     1441 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1501 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1561 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1621 ctggggcaca agctggagta caacttcaac agccacaacg tctatatcat ggccgacaag
     1681 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     1741 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1801 gacaaccact acctgagcat ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     1861 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1921 tacaagtaa
//
