LOCUS       sequence-205607-       10220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  single sgRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, 
            Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT
  TITLE     Dual gene activation and knockout screen reveals directional 
            dependencies in genetic networks.
  JOURNAL   Nat Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062.
  PUBMED    29334369
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062."
COMMENT     Sequence Label: sgLenti
FEATURES             Location/Qualifiers
     source          1..10220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(36..55)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        227..606
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        608..806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     761..781
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             824..1004
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1051..1176
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1673..1906
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2091..2135
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2433..2550
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2803..2836)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2840..2860
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        4438..4649
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4650..5588
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5545..5565
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5609..6205
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5609..5628)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6105..6125
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6215..6268
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6275..6284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6281..6988
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6426..6444)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6694..6713
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6998..7018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7098..7686
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7151..7171)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7569..7580)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7689..7705)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7690..7706)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8215..8395
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8457..9045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8537..8556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9216..10007,10008..10076))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9839..9858
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10077..10181)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
       61 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
      121 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
      181 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
      241 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      301 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      361 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      421 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      481 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      541 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      601 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      661 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      721 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      781 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
      841 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      901 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      961 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     1021 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     1081 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     1141 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     1201 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1261 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1321 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1381 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1441 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1501 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1561 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1621 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1681 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1741 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1801 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1861 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1921 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1981 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     2041 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2101 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2161 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2221 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2281 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2341 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     2401 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2461 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2521 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2581 gtttggttag taccgggccc gctctagaga tccgacgcgc catctctagg cccgcgccgg
     2641 ccccctcgca cggacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat
     2701 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
     2761 ttttaatact agctacattt tacatgatag gcttggattt ctataacttc gtatagcata
     2821 cattatacga agttataaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
     2881 tgttttgaga ctataagtat cccttggaga accaccttgt tggagacgca ggtgtcactg
     2941 gcgctgttgg tgccggcacc tgacacagca gaggatgcag agcccaagga aacccagaaa
     3001 gccgaaggag ccacccagca cgcaagccag gaacactgtc tggttcaccc ctctggataa
     3061 aacctggatc ctggagtcca tcttgacctt atcaccttca ctcagtagac actgccacag
     3121 ccctgtctca ggggccacca cttgaactac tttctgctcc tcagagaccc tggcctcctg
     3181 gttctcctgc ttcagggtca gtctcatctt gggagaggta ggtcccatca cctcacaggt
     3241 caaagtattg ttgagctgag ccactttcat caccaccagg ttcacttcct gatgcagtgt
     3301 ccctttgtcc agagtcagag tcaggttgcc agaaccagca aactgaagcg agacctgggg
     3361 tatcttgagg gtgagtggga gcgtttcctt cagctggagc ttgaggtctt tggtggactt
     3421 ttgtacggac acctctttgt tctttatgga gaaggagatc cagggctgga agaaagaatc
     3481 cttctctgcc ttccacatca gctctcccca cccgttttcc tctgcaaagt tgagtgggaa
     3541 ggagaactcc gctgactctc cctcactctt ataggccgtg atagctgtgc tctgaaaacc
     3601 cagcactgag agtgtcatgc cgaaccagtt ctttttctgg tccagggtca cggtgcagtt
     3661 ccagaagtcg ctgtcctgaa cccttaggtt ggacatggag agaactttgg aaccactgac
     3721 aactttaccc tttttgtgtt tgcactctgt caaggggtta gagaccttag agttgctatc
     3781 caaggtcagg gtcaggctct gcccttgcaa caggctggta cccggactga aggtcacttt
     3841 gaacacccac aactccacct cctctttcct gttctccagc tcacagatat aagtctgaga
     3901 gtcttccatc ttaagtttat tgatgatgag aggaaacgat cctttctccc atgccccttt
     3961 tttggaatca aaacgatcaa actgcgaagg cgaacctcct ctaattaata cacctttgcc
     4021 atgctgcccc agaatcttcc tctggtcaga gaacttccag gtgaagactg tgatcttctt
     4081 ctgggaactc tcgcagggca gttctgctga ttccccttcc ttccccagca ccagcgtctt
     4141 cccttgagtg acagctagga gttgtgacag ctgcagcagc agcagcagca agcgcctaag
     4201 agagatggct cggcacatgt cacctgcgat cgtttaagag ctatgctgga aacagcatag
     4261 caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     4321 ttttgcggcc gcgtggataa ccgtattacc gccatgcatg gtcacagact agcgtagggg
     4381 ttgcagatcg gaagagcaca cgcgtctgaa ctccagtcac tgcatgtgcc cgtcagtggg
     4441 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     4501 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     4561 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     4621 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     4681 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     4741 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     4801 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     4861 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     4921 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     4981 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     5041 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     5101 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     5161 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     5221 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     5281 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     5341 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgcgc ttttggagta
     5401 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     5461 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     5521 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     5581 catttcaggt gtcgtgagct agcccaccat gaccgagtac aagcccacgg tgcgcctcgc
     5641 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     5701 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     5761 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     5821 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     5881 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     5941 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6001 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6061 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6121 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6181 gtgcatgacc cgcaagcccg gtgccggatc gggagagggc agaggaagtc tgctaacatg
     6241 cggtgacgtc gaggagaatc ctggcccacc ggtcgccacc atggtgagca agggcgagga
     6301 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     6361 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     6421 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     6481 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     6541 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     6601 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     6661 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     6721 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     6781 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     6841 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     6901 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     6961 caccggcggc atggacgagc tgtacaaggg agctgatcca aaaaagaaga gaaaggtata
     7021 gttaattaag tcgagggacc taacatgttt aagggttccg gttccactag gtacaattcg
     7081 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7141 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7201 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7261 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7321 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7381 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7441 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     7501 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7561 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7621 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7681 ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt
     7741 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     7801 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     7861 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7921 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     7981 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     8041 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     8101 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     8161 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     8221 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8281 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8341 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatg
     8401 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8461 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8521 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8581 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8641 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8701 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8761 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8821 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8881 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8941 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9001 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9061 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9121 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9181 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9241 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9301 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9361 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9421 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9481 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9541 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9601 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9661 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9721 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9781 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9841 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9901 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9961 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10021 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10081 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10141 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10201 ccacctgacg tcgacggatc
//
