LOCUS       sequence-205608-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and 
            tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, 
            Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
  TITLE     FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's 
            Disease Modeling.
  JOURNAL   Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 
            10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
  PUBMED    28988985
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.08.026"; journalName: "Stem Cell Reports"; 
            date: "2017-11-14- 14"; volume: "9"; issue: "5"; pages: "1423-1431"
COMMENT     Sequence Label: pDONOR-SNCAe3-A53T
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(56..75)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(137..159)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cagctcctcg cccttgctca ccatggtggc ggcctttgcc aaaatgatga gacagcacaa
       61 taaccagcac gttgcccagg agctgtagga aaaagaagaa ggcatgaaca tggttagcag
      121 aggctctaga gccgccggtc acacgccaga agccgaaccc cgccctgccc cgtccccccc
      181 gaaggcagcc gtccccccgc ggacagcccc gaggctggag agggagaagg ggacggcggc
      241 gcggcgacgc acgaaggccc tccccgccca tttccttcct gccggcgccg caccgcttcg
      301 ccccgcgccc gctagagggg gtgcggcggc gcctcccaga tttcggctcc gcacagattt
      361 gggacaaagg aagtccctgc gccctctcgc acgattacca taaaaggcaa tggctgcggc
      421 tcgccgcgcc tcgacagccg ccggcgctcc gggggccgcc gcgcccctcc cccgagccct
      481 ccccggcccg aggcggcccc gccccgcccg gcacccccac ctgccgccac cccccgcccg
      541 gcacggcgag ccccgcgcca cgccccgtac ggagccccgc acccgaagcc gggccgtgct
      601 cagcaactcg gggagggggg tgcagggggg gttgcagccc gaccgacgcg cccacacccc
      661 ctgct
//
