LOCUS       sequence-205610-       11493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation G484E decreased 
            transport and surface expression relative to wildtype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTG484E-myc
FEATURES             Location/Qualifiers
     source          1..11493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(724..742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        810..914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(915..983,984..1775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1133..1152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1946..2534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2435..2454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2965..3110
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3153..4190
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4188..4207)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4232..4280
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4934..5313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5314..5517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5468..5488
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5514..5538
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5566..5575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6241..6270
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7559..7693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7583..7602)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7637..7656
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7836..7858)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8418..10207
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctcccgctcc tgctcctgct cctgttccac cgtgggtccc tttgcagcca atgcaacttg
       61 gacgtttttg gggtctccgg acaccatctc tatgtcttgg ccctgatcct gagccgcccg
      121 gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat
      181 caccccctct tctttgaggt ccactgccgc cggagccttc tggtccagat gtgtctccct
      241 tctctcctag gccatttcca ggtcctgtac ctggcccctc gtcagacatg attcacacta
      301 aaagagatca atagacatct ttattagacg acgctcagtg aatacaggga gtgcagactc
      361 ctgccccctc caacagcccc cccaccctca tccccttcat ggtcgctgtc agacagatcc
      421 aggtctgaaa attccccatc ctccgaacca tcctcgtcct catcaccaat tactcgcagc
      481 ccggaaaact cccgctgaac atcctcaaga tttgcgtcct gagcctcaag ccaggcctca
      541 aattcctcgt cccccttttt gctggacggt agggatgggg attctcggga cccctcctct
      601 tcctcttcaa ggtcaccaga cagagatgct actggggcaa cggaagaaaa gctgggtgcg
      661 gcctgtgagg atcagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa
      721 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      781 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      841 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      901 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      961 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1021 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1081 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1141 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1201 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1261 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1321 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1381 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1441 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     1501 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1561 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1621 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1681 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1741 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1801 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1861 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1921 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1981 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2041 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2101 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2161 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2221 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2281 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2341 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2401 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2461 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2521 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2581 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     2641 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     2701 tcgcgaacgc cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc gcgtgcggct
     2761 gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt
     2821 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg
     2881 ccctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata
     2941 tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg
     3001 aattcgaaca cgcagatgca gtcggggcgg cgcggtccga ggtccacttc gcatattaag
     3061 gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa cagcgtcaac
     3121 agcgtgccgc agatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt
     3181 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     3241 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     3301 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     3361 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     3421 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     3481 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     3541 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     3601 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     3661 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     3721 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     3781 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     3841 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     3901 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     3961 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     4021 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     4081 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     4141 accgacgccc cagcactcgt ccggatcggg agatggggga ggctaactga aacacggaag
     4201 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     4261 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     4321 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     4381 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     4441 catagccact ggccccgtgg gttagggacg gggtccccca tggggaatgg tttatggttc
     4501 gtgggggtta ttattttggg cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca
     4561 gacccatggt ttttggatgg cctgggcatg gaccgcatgt actggcgcga cacgaacacc
     4621 gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg atttctggcg
     4681 tgccaagcta gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg
     4741 ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa
     4801 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
     4861 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     4921 tgaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     4981 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     5041 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     5101 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     5161 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     5221 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     5281 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat
     5341 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     5401 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     5461 ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     5521 ttagtgaacc gtcagatctc tagaagctgg gtaccagctg ctagcgccac catggagacc
     5581 acccccctga acagtcaaaa gcaactctct gcctgtgagg atggagagga ctgtcaggag
     5641 aatggcgttc ttcaaaaggt tgtacccacc cctggagata aggtggaaag cggccagatt
     5701 tccaacggct acagcgccgt gccatcacct ggagccgggg atgatacacg gcacagcatt
     5761 cccgcaacca ccacgacact ggttgcagag cttcaccagg gcgagaggga gacgtggggc
     5821 aaaaaggtag attttctgct ttccgtgatc ggttacgcgg tcgatctggg gaacgtgtgg
     5881 aggttccctt acatttgtta ccaaaacgga gggggtgcct tcttgctgcc ctacacaatt
     5941 atggcgattt ttgggggtat cccgctcttc tacatggaac tggccttggg tcagtaccac
     6001 cggaacggct gcattagcat ttggcgcaag atatgcccca ttttcaaggg aatcggatac
     6061 gcaatctgta taatcgcgtt ctatattgca tcatattaca acaccatcat ggcctgggcc
     6121 ctttattact tgatcagcag tttcaccgac caattgccgt ggacgagctg taagaactca
     6181 tggaataccg ggaactgtac caactacttc tctgaggaca gctcaggagg tagcgggggt
     6241 gaacagaagc tgattagcga ggaggacttg ggcagtccag gcggaagcgg caacatcact
     6301 tggacactcc atagtacgag ccccgcagag gagttttaca ccaggcacgt actgcagatt
     6361 cataggagca agggtctgca agacctgggc ggtattagct ggcagctcgc attgtgcata
     6421 atgttgatct ttacggtgat ctatttctct atctggaagg gagtaaagac atccggcaag
     6481 gtggtgtggg tgacggcgac cttcccctac attatactca gcgtgttgtt ggtgaggggg
     6541 gcaaccctgc ctggggcctg gcgaggcgtg ctgttctatc tcaagccaaa ctggcagaag
     6601 cttcttgaaa ccggagtgtg gatcgacgcg gccgcgcaaa tcttcttttc cctcggtccc
     6661 ggcttcgggg tccttctggc attcgcaagc tacaacaagt tcaacaataa ctgctaccag
     6721 gacgccctcg tgacaagtgt agtgaactgc atgacctctt tcgtgagcgg gtttgtgatc
     6781 ttcacagtac tcggatatat ggctgagatg aggaacgaag atgttagtga ggttgccaaa
     6841 gacgctggcc cgagcctgtt gttcatcaca tacgccgagg ctatcgcgaa catgcccgca
     6901 tctactttct tcgccatcat ctttttcctg atgctgataa cccttgggct ggatagcacc
     6961 ttcgccggac tggagggtgt catcactgcc gttctcgacg agttccccca tgtgtgggcc
     7021 aagcgacggg aaaggttcgt gctggccgtc gtaatcacat gcttcttcgg ctcactggtg
     7081 acccttacct ttgagggtgc gtacgtggtc aagctgctcg aagagtacgc cactggaccg
     7141 gcggttctga ccgtggcatt gatcgaggcc gtggccgtaa gctggtttta cggcatcacg
     7201 cagttctgta gggacgtgaa agagatgctg ggcttttctc ccggttggtt ttggaggatt
     7261 tgttgggtag caattagccc cctgtttctc cttttcatta tttgctcttt tctgatgtct
     7321 ccaccgcagc tccgactgtt tcagtacaac tacccctact ggtcaattat cctgggctat
     7381 tgtatcggca cctcaagttt catctgcatc cccacataca tcgcatacag actgataatt
     7441 acgcccggta cttttaagga gcgaatcatt aaaagcataa cccccgaaac ccccaccgag
     7501 atcccttgtg gcgatataag actcaatgcg gtctaatagc tcgaggccgg caaggccgga
     7561 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7621 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     7681 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     7741 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     7801 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     7861 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     7921 gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga tcgatccccg
     7981 ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg
     8041 taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg
     8101 ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg
     8161 cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc
     8221 aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga
     8281 catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg
     8341 gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg
     8401 cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca
     8461 gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg
     8521 gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt
     8581 atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg
     8641 ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc
     8701 ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc
     8761 gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg
     8821 cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta
     8881 ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta
     8941 aagactggat gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca
     9001 atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt
     9061 tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac
     9121 tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac
     9181 aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca
     9241 cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac
     9301 cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa
     9361 tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac
     9421 aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga
     9481 ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct
     9541 ggatagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     9601 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     9661 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     9721 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta
     9781 gggtagtata tgctatccta atttatatct gggtagcata tactacccaa atatctggat
     9841 agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     9901 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     9961 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
    10021 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
    10081 agcatatgct atcctcatgc atatacagtc agcatatgat acccagtagt agagtgggag
    10141 tgctatcctt tgcatatgcc gccacctccc aagggggcgt gaattttcgc tgcttgtcct
    10201 tttcctgctg gttgctccca ttcttaggtg aatttaagga ggccaggcta aagccgtcgc
    10261 atgtctgatt gctcaccagg taaatgtcgc taatgttttc caacgcgaga aggtgttgag
    10321 cgcggagctg agtgacgtga caacatgggt atgcccaatt gccccatgtt gggaggacga
    10381 aaatggtgac aagacagatg gccagaaata caccaacagc acgcatgatg tctactgggg
    10441 atttattctt tagtgcgggg gaatacacgg cttttaatac gattgagggc gtctcctaac
    10501 aagttacatc actcctgccc ttcctcaccc tcatctccat cacctccttc atctccgtca
    10561 tctccgtcat caccctccgc ggcagcccct tccaccatag gtggaaacca gggaggcaaa
    10621 tctactccat cgtcaaagct gcacacagtc accctgatat tgcaggtagg agcgggcttt
    10681 gtcataacaa ggtccttaat cgcatccttc aaaacctcag caaatatatg agtttgtaaa
    10741 aagaccatga aataacagac aatggactcc cttagcgggc caggttgtgg gccgggtcca
    10801 ggggccattc caaaggggag acgactcaat ggtgtaagac gacattgtgg aatagcaagg
    10861 gcagttcctc gccttaggtt gtaaagggag gtcttactac ctccatatac gaacacaccg
    10921 gcgacccaag ttccttcgtc ggtagtcctt tctacgtgac tcctagccag gagagctctt
    10981 aaaccttctg caatgttctc aaatttcggg ttggaacctc cttgaccacg atgctttcca
    11041 aaccaccctc cttttttgcg cctgcctcca tcaccctgac cccggggtcc agtgcttggg
    11101 ccttctcctg ggtcatctgc ggggccctgc tctatcgctc ccgggggcac gtcaggctca
    11161 ccatctgggc caccttcttg gtggtattca aaataatcgg cttcccctac agggtggaaa
    11221 aatggccttc tacctggagg gggcctgcgc ggtggagacc cggatgatga tgactgacta
    11281 ctgggactcc tgggcctctt ttctccacgt ccacgacctc tccccctggc tctttcacga
    11341 cttccccccc tggctctttc acgtcctcta ccccggcggc ctccactacc tcctcgaccc
    11401 cggcctccac tacctcctcg accccggcct ccactgcctc ctcgaccccg gcctccacct
    11461 cctgctcctg cccctcctgc tcctgcccct cct
//
