LOCUS       sequence-205612-        8758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Streptococcus pyogenes and Staphylococcus aureus sgRNA 
            expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, 
            Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT
  TITLE     Dual gene activation and knockout screen reveals directional 
            dependencies in genetic networks.
  JOURNAL   Nat Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062.
  PUBMED    29334369
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062."
COMMENT     Sequence Label: sgLenti-orthogonal
FEATURES             Location/Qualifiers
     source          1..8758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(48..123)
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     promoter        complement(249..498)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(307..326)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(478..498)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1004..1024)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    1028..1061
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1314..1431
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1729..1773)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1958..2191
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2688..2813
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2860..3040
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3058..3256)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3083..3103)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3258..3637
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3809..3828
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3903..4007
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4008..4076,4077..4868)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4226..4245)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5039..5627
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5528..5547
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             5689..5869
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     6378..6394
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6379..6395
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6398..6986
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6504..6515
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6913..6933
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7066..7086)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(7096..7803)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7371..7390)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7640..7658
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      7800..7809
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7816..7869)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(7879..8475)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7959..7979)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8456..8475
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8488..8699)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gacgtgtgct cttccgatct accggcaatc acgtatcgac gcaaaaatct cgccaacaag
       61 ttgacgagat aaacacggca ttttgccttg ttttagtaga ttctgtttcc agagtactaa
      121 aacgatcgca ggtacatgtg ccgagccatc tctcttaggc gcttgctgct gctgctgcgg
      181 cgcgcctcac aactcctagc tgtcactcaa gggaagacgc tggtgctggg gaagtacctg
      241 cgtctcacgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt
      301 caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac
      361 ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc
      421 aaattaattc taattatctc tctaacagac cttgtatcgt atatgcaaat atgaaggaat
      481 catgggaaat aggccctctt cctgcccgac cgctacctag ggctcatcac cggcctctgg
      541 ggcaaggtca atgtggccga atgtggggcc gaagccctgg ccaggctgct gatcgtctac
      601 ccctggaccc agaggttctt tgcgtccttt gggaacctct ccagccccac tgccatcctt
      661 ggcaacccca tggtccgcgc ccacggcaag aaagtgctca cctcctttgg ggatgctgtg
      721 catgcaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat
      781 ttaaacttgc tatgctgttt ccagcatagc tcttaaacga tcgcaggtgg ttcctggctt
      841 gcgtgctggg tggctccttc ggctttggcg cgccccttgg gctctgcatc ctctgctgtg
      901 tcaggtgccg gcaccaacag cgccagtgac acctgcgtct ccaacaaggt ggttctccaa
      961 gggatactta tagtctcaaa acacacaatt actttacagt tagggtgagt ttccttttgt
     1021 gctgtttata acttcgtata atgtatgcta tacgaagtta tagaaatcca agcctatcat
     1081 gtaaaatgta gctagtatta aaaagaacag attatctgtc ttttatcgca cattaagcct
     1141 ctatagttac taggaaatat tatatgcaaa ttaaccgggg caggggagta gccgagcttc
     1201 tcccacaagt ccgtgcgagg gggccggcgc gggcctagag atggcgcgtc ggatctctag
     1261 agcgggcccg gtactaacca aactggatct ctgctgtccc tgtaataaac ccgaaaattt
     1321 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
     1381 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg
     1441 aatactgcca tttgtctgca gaattggcgc acgcagtgcc gatccgttca ctaatcgaat
     1501 ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc
     1561 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     1621 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     1681 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     1741 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     1801 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     1861 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     1921 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     1981 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     2041 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     2101 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     2161 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     2221 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     2281 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
     2341 cggccggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
     2401 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     2461 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     2521 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
     2581 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     2641 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     2701 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     2761 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     2821 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     2881 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     2941 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     3001 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaacgcgctg
     3061 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     3121 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     3181 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     3241 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     3301 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     3361 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     3421 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     3481 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     3541 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     3601 attgattact attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca
     3661 tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag
     3721 ccttgccttg ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa
     3781 gcagggagca gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     3841 cataggggat cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg
     3901 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3961 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4021 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4081 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4141 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4201 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4261 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4321 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4381 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4441 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4501 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4561 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4621 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4681 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4741 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4801 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4861 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4921 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4981 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5041 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5101 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5161 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5221 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5281 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5341 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5401 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5461 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5521 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5581 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5641 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgctg ctagagattt
     5701 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     5761 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     5821 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacagtccg
     5881 gatgcagctc tcgggccatg tgatgaaatg ctaggcggct gtcaaacctc cactctaata
     5941 cttctctctc cgggtcatcc atcccatgca ggctcacagg gtgtaacaag cgggtgttct
     6001 ctccttcatt ggcttcttct accttctctt gctcaactgg tactagcttg tagcaccatc
     6061 caaaggtcag tggatatctg atccctggcc ctggtgtgta gttctgccaa tcagggaagt
     6121 agccttgtgt gtggtagatc cacagatcaa ggatatcttg tcttcgttgg gagtgaatta
     6181 gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag
     6241 tcattggtct taaaggtacc tgaggtgtga ctggaaaacc cacctcctcc tcctcttgtg
     6301 cttctagcca ggcacaatca gcattggtag ctgctgtatt gctacttgtg attgctccat
     6361 gtttttctag gtctcgatcg aggtcgacgg tatcgatgcg gggaggcggc ccaaagggag
     6421 atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg
     6481 ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg
     6541 cgcagaatcc aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac
     6601 aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag
     6661 gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc
     6721 tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc
     6781 acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta
     6841 tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa
     6901 gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca
     6961 caaattttgt aatccagagg ttgattatcg ataagcttga tatcgaattg tacctagtgg
     7021 aaccggaacc cttaaacatg ttaggtccct cgacttaatt aactatacct ttctcttctt
     7081 ttttggatca gctcccttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
     7141 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
     7201 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
     7261 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
     7321 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
     7381 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
     7441 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
     7501 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
     7561 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
     7621 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
     7681 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
     7741 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac
     7801 catggtggcg accggtgggc caggattctc ctcgacgtca ccgcatgtta gcagacttcc
     7861 tctgccctct cccgatccgg caccgggctt gcgggtcatg caccaggtgc gcggtccttc
     7921 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg
     7981 gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg
     8041 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc
     8101 caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg
     8161 cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc
     8221 ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac
     8281 ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac
     8341 ccgctcgatg tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt cggcgaacgc
     8401 ggcggcgagg gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc gcaccgtggg
     8461 cttgtactcg gtcatggtgg gctagctcac tgttctggcg gcaaacccgt tgcgaaaaag
     8521 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
     8581 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt
     8641 tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca
     8701 ctgacgggca catgcatggc ggtaatacgg ttatccacgc gtgactggag ttcagacg
//
