LOCUS       sequence-205614-       11512 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation L550V approximately 
            wildtype transport.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTL550V-myc
FEATURES             Location/Qualifiers
     source          1..11512
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(723..741)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        809..913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(914..982,983..1774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1132..1151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1945..2533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2434..2453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2964..3109
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3152..4189
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4187..4206)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4231..4279
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4933..5312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5313..5516
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5467..5487
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5513..5537
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5565..5574
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6240..6269
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7558..7692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7582..7601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7636..7655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7835..7857)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8417..10206
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg
       61 acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg
      121 ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc
      181 accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt
      241 ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa
      301 aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc
      361 tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca
      421 ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc
      481 cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa
      541 attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt
      601 cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg
      661 cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
      721 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      781 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      841 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      901 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      961 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1021 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1081 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1141 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     1201 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1261 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1321 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1381 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1441 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1501 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1561 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1621 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1681 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1741 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1801 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1861 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1921 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1981 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2041 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     2101 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2161 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2221 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2281 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2341 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2401 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2461 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2521 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2581 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
     2641 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
     2701 cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg
     2761 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
     2821 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
     2881 cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat
     2941 atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga
     3001 attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg
     3061 tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca
     3121 gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
     3181 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     3241 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     3301 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     3361 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     3421 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     3481 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     3541 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     3601 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     3661 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     3721 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     3781 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     3841 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     3901 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     3961 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     4021 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     4081 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     4141 ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg
     4201 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     4261 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     4321 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     4381 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     4441 atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg
     4501 tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag
     4561 acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg
     4621 ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt
     4681 gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     4741 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     4801 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     4861 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     4921 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     4981 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     5041 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     5101 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     5161 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
     5221 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
     5281 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
     5341 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5401 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
     5461 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     5521 tagtgaaccg tcagatctct agaagctggg taccagctgc tagcgccacc atggagacca
     5581 cccccctgaa cagtcaaaag caactctctg cctgtgagga tggagaggac tgtcaggaga
     5641 atggcgttct tcaaaaggtt gtacccaccc ctggagataa ggtggaaagc ggccagattt
     5701 ccaacggcta cagcgccgtg ccatcacctg gagccgggga tgatacacgg cacagcattc
     5761 ccgcaaccac cacgacactg gttgcagagc ttcaccaggg cgagagggag acgtggggca
     5821 aaaaggtaga ttttctgctt tccgtgatcg gttacgcggt cgatctgggg aacgtgtgga
     5881 ggttccctta catttgttac caaaacggag ggggtgcctt cttgctgccc tacacaatta
     5941 tggcgatttt tgggggtatc ccgctcttct acatggaact ggccttgggt cagtaccacc
     6001 ggaacggctg cattagcatt tggcgcaaga tatgccccat tttcaaggga atcggatacg
     6061 caatctgtat aatcgcgttc tatattgcat catattacaa caccatcatg gcctgggccc
     6121 tttattactt gatcagcagt ttcaccgacc aattgccgtg gacgagctgt aagaactcat
     6181 ggaataccgg gaactgtacc aactacttct ctgaggacag ctcaggaggt agcgggggtg
     6241 aacagaagct gattagcgag gaggacttgg gcagtccagg cggaagcggc aacatcactt
     6301 ggacactcca tagtacgagc cccgcagagg agttttacac caggcacgta ctgcagattc
     6361 ataggagcaa gggtctgcaa gacctgggcg gtattagctg gcagctcgca ttgtgcataa
     6421 tgttgatctt tacggtgatc tatttctcta tctggaaggg agtaaagaca tccggcaagg
     6481 tggtgtgggt gacggcgacc ttcccctaca ttatactcag cgtgttgttg gtgagggggg
     6541 caaccctgcc tggggcctgg cgaggcgtgc tgttctatct caagccaaac tggcagaagc
     6601 ttcttgaaac cggagtgtgg atcgacgcgg ccgcgcaaat cttcttttcc ctcggtcccg
     6661 gcttcggggt ccttctggca ttcgcaagct acaacaagtt caacaataac tgctaccagg
     6721 acgccctcgt gacaagtgta gtgaactgca tgacctcttt cgtgagcggg tttgtgatct
     6781 tcacagtact cggatatatg gctgagatga ggaacgaaga tgttagtgag gttgccaaag
     6841 acgctggccc gagcctgttg ttcatcacat acgccgaggc tatcgcgaac atgcccgcat
     6901 ctactttctt cgccatcatc tttttcctga tgctgataac ccttgggctg gatagcacct
     6961 tcgccggact ggagggtgtc atcactgccg ttctcgacga gttcccccat gtgtgggcca
     7021 agcgacggga aaggttcgtg ctggccgtcg taatcacatg cttcttcggc tcactggtga
     7081 cccttacctt tggcggtgcg tacgtggtca agctgctcga agagtacgcc actggaccgg
     7141 cggttctgac cgtggcattg atcgaggccg tggccgtaag ctggttttac ggcatcacgc
     7201 agttctgtag ggacgtgaaa gagatgctgg gcttttctcc cggttggttt tggaggattt
     7261 gttgggtagc aattagcccc ctgtttctcg tcttcattat ttgctctttt ctgatgtctc
     7321 caccgcagct ccgactgttt cagtacaact acccctactg gtcaattatc ctgggctatt
     7381 gtatcggcac ctcaagtttc atctgcatcc ccacatacat cgcatacaga ctgataatta
     7441 cgcccggtac ttttaaggag cgaatcatta aaagcataac ccccgaaacc cccaccgaga
     7501 tcccttgtgg cgatataaga ctcaatgcgg tctaatagct cgaggccggc aaggccggat
     7561 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     7621 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     7681 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     7741 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     7801 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7861 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7921 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc
     7981 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     8041 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     8101 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     8161 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     8221 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     8281 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     8341 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     8401 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     8461 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     8521 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     8581 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     8641 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     8701 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     8761 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     8821 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     8881 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     8941 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     9001 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     9061 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     9121 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     9181 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     9241 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     9301 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     9361 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     9421 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     9481 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     9541 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     9601 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     9661 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     9721 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     9781 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     9841 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     9901 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     9961 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
    10021 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
    10081 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
    10141 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
    10201 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
    10261 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
    10321 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
    10381 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
    10441 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
    10501 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
    10561 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
    10621 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
    10681 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
    10741 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
    10801 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
    10861 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
    10921 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
    10981 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
    11041 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
    11101 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
    11161 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
    11221 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
    11281 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
    11341 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
    11401 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
    11461 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gc
//
