LOCUS       sequence-205619-       11457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation N177E with approximately
            wildtype activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTN177E-myc
FEATURES             Location/Qualifiers
     source          1..11457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(697..715)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        783..887
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(888..956,957..1748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1106..1125)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1919..2507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2408..2427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2938..3083
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3126..4163
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4161..4180)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4205..4253
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4907..5286
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5287..5490
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5441..5461
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5487..5511
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5539..5548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6214..6243
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7532..7666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7556..7575)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7610..7629
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7809..7831)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8391..10180
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 caccgtgggt ccctttgcag ccaatgcaac ttggacgttt ttggggtctc cggacaccat
       61 ctctatgtct tggccctgat cctgagccgc ccggggctcc tggtcttccg cctcctcgtc
      121 ctcgtcctct tccccgtcct cgtccatggt tatcaccccc tcttctttga ggtccactgc
      181 cgccggagcc ttctggtcca gatgtgtctc ccttctctcc taggccattt ccaggtcctg
      241 tacctggccc ctcgtcagac atgattcaca ctaaaagaga tcaatagaca tctttattag
      301 acgacgctca gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc
      361 tcatcccctt catggtcgct gtcagacaga tccaggtctg aaaattcccc atcctccgaa
      421 ccatcctcgt cctcatcacc aattactcgc agcccggaaa actcccgctg aacatcctca
      481 agatttgcgt cctgagcctc aagccaggcc tcaaattcct cgtccccctt tttgctggac
      541 ggtagggatg gggattctcg ggacccctcc tcttcctctt caaggtcacc agacagagat
      601 gctactgggg caacggaaga aaagctgggt gcggcctgtg aggatcagct tatcgatgat
      661 aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
      721 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      781 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      841 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      901 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      961 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1021 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1081 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     1141 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1201 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1261 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1321 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1381 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     1441 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1501 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1561 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1621 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1681 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1741 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1801 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1861 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1921 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1981 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2041 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     2101 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2161 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2221 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2281 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2341 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2401 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2461 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2521 cggccttttt acggttcctg gccttttgct ggccttgaag ctgtccctga tggtcgtcat
     2581 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
     2641 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
     2701 gcgcgtcggc cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat
     2761 gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat
     2821 tcttggagtg gtgaatccgt tagcgaggtg ccgccctgct tcatccccgt ggcccgttgc
     2881 tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag ttctatgatg
     2941 acacaaaccc cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg
     3001 cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag
     3061 cgaccctgca gcgacccgct taacagcgtc aacagcgtgc cgcagatccc ggggggcaat
     3121 gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     3181 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     3241 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     3301 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     3361 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     3421 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     3481 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     3541 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     3601 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     3661 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     3721 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     3781 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     3841 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     3901 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     3961 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     4021 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     4081 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccggatc
     4141 gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     4201 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
     4261 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     4321 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     4381 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc actggccccg tgggttaggg
     4441 acggggtccc ccatggggaa tggtttatgg ttcgtggggg ttattatttt gggcgttgcg
     4501 tggggtcagg tccacgactg gactgagcag acagacccat ggtttttgga tggcctgggc
     4561 atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc aaacaccccc
     4621 gacccccaaa aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc aattctcatg
     4681 tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac
     4741 tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat attagtcatt
     4801 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat
     4861 aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttgaca ttgattattg
     4921 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     4981 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     5041 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     5101 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     5161 ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     5221 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     5281 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
     5341 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     5401 cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt
     5461 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat ctctagaagc
     5521 tgggtaccag ctgctagcgc caccatggag accacccccc tgaacagtca aaagcaactc
     5581 tctgcctgtg aggatggaga ggactgtcag gagaatggcg ttcttcaaaa ggttgtaccc
     5641 acccctggag ataaggtgga aagcggccag atttccaacg gctacagcgc cgtgccatca
     5701 cctggagccg gggatgatac acggcacagc attcccgcaa ccaccacgac actggttgca
     5761 gagcttcacc agggcgagag ggagacgtgg ggcaaaaagg tagattttct gctttccgtg
     5821 atcggttacg cggtcgatct ggggaacgtg tggaggttcc cttacatttg ttaccaaaac
     5881 ggagggggtg ccttcttgct gccctacaca attatggcga tttttggggg tatcccgctc
     5941 ttctacatgg aactggcctt gggtcagtac caccggaacg gctgcattag catttggcgc
     6001 aagatatgcc ccattttcaa gggaatcgga tacgcaatct gtataatcgc gttctatatt
     6061 gcatcatatt acgaaaccat catggcctgg gccctttatt acttgatcag cagtttcacc
     6121 gaccaattgc cgtggacgag ctgtaagaac tcatggaata ccgggaactg taccaactac
     6181 ttctctgagg acagctcagg aggtagcggg ggtgaacaga agctgattag cgaggaggac
     6241 ttgggcagtc caggcggaag cggcaacatc acttggacac tccatagtac gagccccgca
     6301 gaggagtttt acaccaggca cgtactgcag attcatagga gcaagggtct gcaagacctg
     6361 ggcggtatta gctggcagct cgcattgtgc ataatgttga tctttacggt gatctatttc
     6421 tctatctgga agggagtaaa gacatccggc aaggtggtgt gggtgacggc gaccttcccc
     6481 tacattatac tcagcgtgtt gttggtgagg ggggcaaccc tgcctggggc ctggcgaggc
     6541 gtgctgttct atctcaagcc aaactggcag aagcttcttg aaaccggagt gtggatcgac
     6601 gcggccgcgc aaatcttctt ttccctcggt cccggcttcg gggtccttct ggcattcgca
     6661 agctacaaca agttcaacaa taactgctac caggacgccc tcgtgacaag tgtagtgaac
     6721 tgcatgacct ctttcgtgag cgggtttgtg atcttcacag tactcggata tatggctgag
     6781 atgaggaacg aagatgttag tgaggttgcc aaagacgctg gcccgagcct gttgttcatc
     6841 acatacgccg aggctatcgc gaacatgccc gcatctactt tcttcgccat catctttttc
     6901 ctgatgctga taacccttgg gctggatagc accttcgccg gactggaggg tgtcatcact
     6961 gccgttctcg acgagttccc ccatgtgtgg gccaagcgac gggaaaggtt cgtgctggcc
     7021 gtcgtaatca catgcttctt cggctcactg gtgaccctta cctttggcgg tgcgtacgtg
     7081 gtcaagctgc tcgaagagta cgccactgga ccggcggttc tgaccgtggc attgatcgag
     7141 gccgtggccg taagctggtt ttacggcatc acgcagttct gtagggacgt gaaagagatg
     7201 ctgggctttt ctcccggttg gttttggagg atttgttggg tagcaattag ccccctgttt
     7261 ctccttttca ttatttgctc ttttctgatg tctccaccgc agctccgact gtttcagtac
     7321 aactacccct actggtcaat tatcctgggc tattgtatcg gcacctcaag tttcatctgc
     7381 atccccacat acatcgcata cagactgata attacgcccg gtacttttaa ggagcgaatc
     7441 attaaaagca taacccccga aacccccacc gagatccctt gtggcgatat aagactcaat
     7501 gcggtctaat agctcgaggc cggcaaggcc ggatccagac atgataagat acattgatga
     7561 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     7621 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     7681 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     7741 cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac
     7801 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     7861 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     7921 gccatgaggt cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga
     7981 catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact
     8041 tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc
     8101 tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc
     8161 atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact
     8221 cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga
     8281 ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc
     8341 ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc
     8401 atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt
     8461 tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc
     8521 gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag
     8581 ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac
     8641 tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg
     8701 agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc
     8761 agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc
     8821 tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa
     8881 caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat
     8941 ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg
     9001 tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga
     9061 aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt
     9121 aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg
     9181 aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta
     9241 aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact
     9301 tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca
     9361 agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc
     9421 caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg
     9481 ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     9541 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     9601 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     9661 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
     9721 tccgggtagc atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata
     9781 tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     9841 ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     9901 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     9961 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
    10021 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca
    10081 gtcagcatat gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct
    10141 cccaaggggg cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag
    10201 gtgaatttaa ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt
    10261 cgctaatgtt ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg
    10321 ggtatgccca attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa
    10381 atacaccaac agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca
    10441 cggcttttaa tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca
    10501 ccctcatctc catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc
    10561 ccttccacca taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca
    10621 gtcaccctga tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc
    10681 ttcaaaacct cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac
    10741 tcccttagcg ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc
    10801 aatggtgtaa gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg
    10861 gaggtcttac tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc
    10921 ctttctacgt gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc
    10981 gggttggaac ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct
    11041 ccatcaccct gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc
    11101 tgctctatcg ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat
    11161 tcaaaataat cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg
    11221 cgcggtggag acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca
    11281 cgtccacgac ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct
    11341 ctaccccggc ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg
    11401 cctccactgc ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcct
//
