LOCUS       sequence-205620-       11423 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation R79V conferring gain of 
            function phenotype, decreased surface expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTR79V-myc
FEATURES             Location/Qualifiers
     source          1..11423
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(660..678)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        746..850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(851..919,920..1711)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1069..1088)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1882..2470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2371..2390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2901..3046
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3089..4126
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4124..4143)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4168..4216
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4870..5249
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5250..5453
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5404..5424
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5450..5474
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5502..5511
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6177..6206
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7495..7629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7519..7538)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7573..7592
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7772..7794)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8354..10143
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc
       61 tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc
      121 ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc
      181 tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag
      241 agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc
      301 cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt
      361 ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg
      421 aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt
      481 cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct
      541 cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct
      601 gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg
      661 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      721 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      781 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      841 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      901 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      961 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1021 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1081 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1141 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1201 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1261 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1321 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1381 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1441 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1501 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1561 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1621 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1681 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1741 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1801 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1861 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1921 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1981 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2041 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2101 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2161 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2221 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2281 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2341 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2401 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2461 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg
     2521 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     2581 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     2641 gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg
     2701 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     2761 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct
     2821 gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt
     2881 tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt
     2941 cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga
     3001 cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg
     3061 tgccgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt
     3121 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     3181 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     3241 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     3301 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     3361 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     3421 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     3481 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     3541 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     3601 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     3661 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     3721 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     3781 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     3841 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     3901 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     3961 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     4021 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     4081 acgccccagc actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga
     4141 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
     4201 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     4261 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     4321 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     4381 gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg
     4441 gggttattat tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc
     4501 catggttttt ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc
     4561 gtctgtggct gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc
     4621 aagctagtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
     4681 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
     4741 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
     4801 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
     4861 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     4921 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     4981 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     5041 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     5101 cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat
     5161 ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca
     5221 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc
     5281 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     5341 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt
     5401 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     5461 tgaaccgtca gatctctaga agctgggtac cagctgctag cgccaccatg gagaccaccc
     5521 ccctgaacag tcaaaagcaa ctctctgcct gtgaggatgg agaggactgt caggagaatg
     5581 gcgttcttca aaaggttgta cccacccctg gagataaggt ggaaagcggc cagatttcca
     5641 acggctacag cgccgtgcca tcacctggag ccggggatga tacacggcac agcattcccg
     5701 caaccaccac gacactggtt gcagagcttc accagggcga ggtagagacg tggggcaaaa
     5761 aggtagattt tctgctttcc gtgatcggtt acgcggtcga tctggggaac gtgtggaggt
     5821 tcccttacat ttgttaccaa aacggagggg gtgccttctt gctgccctac acaattatgg
     5881 cgatttttgg gggtatcccg ctcttctaca tggaactggc cttgggtcag taccaccgga
     5941 acggctgcat tagcatttgg cgcaagatat gccccatttt caagggaatc ggatacgcaa
     6001 tctgtataat cgcgttctat attgcatcat attacaacac catcatggcc tgggcccttt
     6061 attacttgat cagcagtttc accgaccaat tgccgtggac gagctgtaag aactcatgga
     6121 ataccgggaa ctgtaccaac tacttctctg aggacagctc aggaggtagc gggggtgaac
     6181 agaagctgat tagcgaggag gacttgggca gtccaggcgg aagcggcaac atcacttgga
     6241 cactccatag tacgagcccc gcagaggagt tttacaccag gcacgtactg cagattcata
     6301 ggagcaaggg tctgcaagac ctgggcggta ttagctggca gctcgcattg tgcataatgt
     6361 tgatctttac ggtgatctat ttctctatct ggaagggagt aaagacatcc ggcaaggtgg
     6421 tgtgggtgac ggcgaccttc ccctacatta tactcagcgt gttgttggtg aggggggcaa
     6481 ccctgcctgg ggcctggcga ggcgtgctgt tctatctcaa gccaaactgg cagaagcttc
     6541 ttgaaaccgg agtgtggatc gacgcggccg cgcaaatctt cttttccctc ggtcccggct
     6601 tcggggtcct tctggcattc gcaagctaca acaagttcaa caataactgc taccaggacg
     6661 ccctcgtgac aagtgtagtg aactgcatga cctctttcgt gagcgggttt gtgatcttca
     6721 cagtactcgg atatatggct gagatgagga acgaagatgt tagtgaggtt gccaaagacg
     6781 ctggcccgag cctgttgttc atcacatacg ccgaggctat cgcgaacatg cccgcatcta
     6841 ctttcttcgc catcatcttt ttcctgatgc tgataaccct tgggctggat agcaccttcg
     6901 ccggactgga gggtgtcatc actgccgttc tcgacgagtt cccccatgtg tgggccaagc
     6961 gacgggaaag gttcgtgctg gccgtcgtaa tcacatgctt cttcggctca ctggtgaccc
     7021 ttacctttgg cggtgcgtac gtggtcaagc tgctcgaaga gtacgccact ggaccggcgg
     7081 ttctgaccgt ggcattgatc gaggccgtgg ccgtaagctg gttttacggc atcacgcagt
     7141 tctgtaggga cgtgaaagag atgctgggct tttctcccgg ttggttttgg aggatttgtt
     7201 gggtagcaat tagccccctg tttctccttt tcattatttg ctcttttctg atgtctccac
     7261 cgcagctccg actgtttcag tacaactacc cctactggtc aattatcctg ggctattgta
     7321 tcggcacctc aagtttcatc tgcatcccca catacatcgc atacagactg ataattacgc
     7381 ccggtacttt taaggagcga atcattaaaa gcataacccc cgaaaccccc accgagatcc
     7441 cttgtggcga tataagactc aatgcggtct aatagctcga ggccggcaag gccggatcca
     7501 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     7561 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     7621 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     7681 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatccggct
     7741 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     7801 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     7861 gtgttggcgg gtgtcggggc gcagccatga ggtcgactct agaggatcga tccccgcccc
     7921 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     7981 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     8041 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     8101 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     8161 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     8221 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     8281 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     8341 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     8401 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     8461 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     8521 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     8581 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     8641 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     8701 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     8761 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     8821 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     8881 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     8941 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     9001 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     9061 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     9121 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     9181 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     9241 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     9301 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     9361 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     9421 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     9481 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     9541 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     9601 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     9661 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     9721 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     9781 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     9841 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     9901 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     9961 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
    10021 tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag tgggagtgct
    10081 atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct tgtccttttc
    10141 ctgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt
    10201 ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg
    10261 gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga ggacgaaaat
    10321 ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta ctggggattt
    10381 attctttagt gcgggggaat acacggcttt taatacgatt gagggcgtct cctaacaagt
    10441 tacatcactc ctgcccttcc tcaccctcat ctccatcacc tccttcatct ccgtcatctc
    10501 cgtcatcacc ctccgcggca gccccttcca ccataggtgg aaaccaggga ggcaaatcta
    10561 ctccatcgtc aaagctgcac acagtcaccc tgatattgca ggtaggagcg ggctttgtca
    10621 taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga
    10681 ccatgaaata acagacaatg gactccctta gcgggccagg ttgtgggccg ggtccagggg
    10741 ccattccaaa ggggagacga ctcaatggtg taagacgaca ttgtggaata gcaagggcag
    10801 ttcctcgcct taggttgtaa agggaggtct tactacctcc atatacgaac acaccggcga
    10861 cccaagttcc ttcgtcggta gtcctttcta cgtgactcct agccaggaga gctcttaaac
    10921 cttctgcaat gttctcaaat ttcgggttgg aacctccttg accacgatgc tttccaaacc
    10981 accctccttt tttgcgcctg cctccatcac cctgaccccg gggtccagtg cttgggcctt
    11041 ctcctgggtc atctgcgggg ccctgctcta tcgctcccgg gggcacgtca ggctcaccat
    11101 ctgggccacc ttcttggtgg tattcaaaat aatcggcttc ccctacaggg tggaaaaatg
    11161 gccttctacc tggagggggc ctgcgcggtg gagacccgga tgatgatgac tgactactgg
    11221 gactcctggg cctcttttct ccacgtccac gacctctccc cctggctctt tcacgacttc
    11281 cccccctggc tctttcacgt cctctacccc ggcggcctcc actacctcct cgaccccggc
    11341 ctccactacc tcctcgaccc cggcctccac tgcctcctcg accccggcct ccacctcctg
    11401 ctcctgcccc tcctgctcct gcc
//
