LOCUS       sequence-205623-       12145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation F88H conferring gain of 
            function phenotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 12145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTF88H-myc
FEATURES             Location/Qualifiers
     source          1..12145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    14..148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(38..57)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     92..111
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(291..313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      873..2662
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2964..4889)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(5324..5342)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5410..5514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5515..5583,5584..6375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5733..5752)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6546..7134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7035..7054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7565..7710
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7753..8790
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(8788..8807)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8832..8880
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9534..9913
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9914..10117
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10068..10088
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10114..10138
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      10166..10175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10841..10870
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gccggcaagg ccggatccag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      181 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
      241 ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta
      421 gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc
      481 gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt
      541 ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc
      601 ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg
      661 cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac
      721 accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca
      781 acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca
      841 atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt
      901 agtatatact atccagacta accctaattc aatagcatat gttacccaac gggaagcata
      961 tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg
     1021 agctgtcacg gttttattta catggggtca ggattccacg agggtagtga accattttag
     1081 tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct
     1141 gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt
     1201 gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg
     1261 ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca
     1321 ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg
     1381 tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca
     1441 cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag
     1501 acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac
     1561 agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca
     1621 atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc
     1681 acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac
     1741 ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa
     1801 tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc
     1861 tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg
     1921 tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat
     1981 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta
     2041 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     2101 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     2161 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     2221 tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta
     2281 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct
     2341 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     2401 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     2461 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     2521 aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca
     2581 gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt
     2641 ttcgctgctt gtccttttcc tgctggttgc tcccattctt aggtgaattt aaggaggcca
     2701 ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg ttttccaacg
     2761 cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc caattgcccc
     2821 atgttgggag gacgaaaatg gtgacaagac agatggccag aaatacacca acagcacgca
     2881 tgatgtctac tggggattta ttctttagtg cgggggaata cacggctttt aatacgattg
     2941 agggcgtctc ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct
     3001 ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga
     3061 aaccagggag gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag
     3121 gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat
     3181 atatgagttt gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt
     3241 tgtgggccgg gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat
     3301 tgtggaatag caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca
     3361 tatacgaaca caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta
     3421 gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga
     3481 ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg
     3541 ggtccagtgc ttgggccttc tcctgggtca tctgcggggc cctgctctat cgctcccggg
     3601 ggcacgtcag gctcaccatc tgggccacct tcttggtggt attcaaaata atcggcttcc
     3661 cctacagggt ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg agacccggat
     3721 gatgatgact gactactggg actcctgggc ctcttttctc cacgtccacg acctctcccc
     3781 ctggctcttt cacgacttcc ccccctggct ctttcacgtc ctctaccccg gcggcctcca
     3841 ctacctcctc gaccccggcc tccactacct cctcgacccc ggcctccact gcctcctcga
     3901 ccccggcctc cacctcctgc tcctgcccct cctgctcctg cccctcctcc tgctcctgcc
     3961 cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
     4021 cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct
     4081 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     4141 gcccctcctg ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc tcctgctcct
     4201 gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgctcc tgcccctcct
     4261 gcccctcctg cccctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     4321 gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     4381 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct
     4441 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct
     4501 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     4561 cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcccgctcc tgctcctgct
     4621 cctgttccac cgtgggtccc tttgcagcca atgcaacttg gacgtttttg gggtctccgg
     4681 acaccatctc tatgtcttgg ccctgatcct gagccgcccg gggctcctgg tcttccgcct
     4741 cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat caccccctct tctttgaggt
     4801 ccactgccgc cggagccttc tggtccagat gtgtctccct tctctcctag gccatttcca
     4861 ggtcctgtac ctggcccctc gtcagacatg attcacacta aaagagatca atagacatct
     4921 ttattagacg acgctcagtg aatacaggga gtgcagactc ctgccccctc caacagcccc
     4981 cccaccctca tccccttcat ggtcgctgtc agacagatcc aggtctgaaa attccccatc
     5041 ctccgaacca tcctcgtcct catcaccaat tactcgcagc ccggaaaact cccgctgaac
     5101 atcctcaaga tttgcgtcct gagcctcaag ccaggcctca aattcctcgt cccccttttt
     5161 gctggacggt agggatgggg attctcggga cccctcctct tcctcttcaa ggtcaccaga
     5221 cagagatgct actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat
     5281 cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta
     5341 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5401 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5461 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5521 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5581 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5641 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5701 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     5761 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5821 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5881 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5941 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6001 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     6061 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6241 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6301 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6361 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6421 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6661 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttgaagctg tccctgatgg
     7201 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
     7261 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg
     7321 tagcccagcg cgtcggcccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga
     7381 tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg
     7441 ctccaattct tggagtggtg aatccgttag cgaggtgccg ccctgcttca tccccgtggc
     7501 ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg tctttagttc
     7561 tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca cgcagatgca
     7621 gtcggggcgg cgcggtccga ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa
     7681 caccgagcga ccctgcagcg acccgcttaa cagcgtcaac agcgtgccgc agatcccggg
     7741 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     7801 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     7861 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     7921 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     7981 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     8041 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     8101 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     8161 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     8221 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     8281 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     8341 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     8401 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     8461 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     8521 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     8581 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     8641 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     8701 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     8761 ccggatcggg agatggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     8821 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
     8881 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     8941 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     9001 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagccact ggccccgtgg
     9061 gttagggacg gggtccccca tggggaatgg tttatggttc gtgggggtta ttattttggg
     9121 cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca gacccatggt ttttggatgg
     9181 cctgggcatg gaccgcatgt actggcgcga cacgaacacc gggcgtctgt ggctgccaaa
     9241 cacccccgac ccccaaaaac caccgcgcgg atttctggcg tgccaagcta gtcgaccaat
     9301 tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc
     9361 gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt
     9421 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     9481 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
     9541 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     9601 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     9661 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     9721 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     9781 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     9841 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     9901 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     9961 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
    10021 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
    10081 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatctc
    10141 tagaagctgg gtaccagctg ctagcgccac catggagacc acccccctga acagtcaaaa
    10201 gcaactctct gcctgtgagg atggagagga ctgtcaggag aatggcgttc ttcaaaaggt
    10261 tgtacccacc cctggagata aggtggaaag cggccagatt tccaacggct acagcgccgt
    10321 gccatcacct ggagccgggg atgatacacg gcacagcatt cccgcaacca ccacgacact
    10381 ggttgcagag cttcaccagg gcgagaggga gacgtggggc aaaaaggtag atcatctgct
    10441 ttccgtgatc ggttacgcgg tcgatctggg gaacgtgtgg aggttccctt acatttgtta
    10501 ccaaaacgga gggggtgcct tcttgctgcc ctacacaatt atggcgattt ttgggggtat
    10561 cccgctcttc tacatggaac tggccttggg tcagtaccac cggaacggct gcattagcat
    10621 ttggcgcaag atatgcccca ttttcaaggg aatcggatac gcaatctgta taatcgcgtt
    10681 ctatattgca tcatattaca acaccatcat ggcctgggcc ctttattact tgatcagcag
    10741 tttcaccgac caattgccgt ggacgagctg taagaactca tggaataccg ggaactgtac
    10801 caactacttc tctgaggaca gctcaggagg tagcgggggt gaacagaagc tgattagcga
    10861 ggaggacttg ggcagtccag gcggaagcgg caacatcact tggacactcc atagtacgag
    10921 ccccgcagag gagttttaca ccaggcacgt actgcagatt cataggagca agggtctgca
    10981 agacctgggc ggtattagct ggcagctcgc attgtgcata atgttgatct ttacggtgat
    11041 ctatttctct atctggaagg gagtaaagac atccggcaag gtggtgtggg tgacggcgac
    11101 cttcccctac attatactca gcgtgttgtt ggtgaggggg gcaaccctgc ctggggcctg
    11161 gcgaggcgtg ctgttctatc tcaagccaaa ctggcagaag cttcttgaaa ccggagtgtg
    11221 gatcgacgcg gccgcgcaaa tcttcttttc cctcggtccc ggcttcgggg tccttctggc
    11281 attcgcaagc tacaacaagt tcaacaataa ctgctaccag gacgccctcg tgacaagtgt
    11341 agtgaactgc atgacctctt tcgtgagcgg gtttgtgatc ttcacagtac tcggatatat
    11401 ggctgagatg aggaacgaag atgttagtga ggttgccaaa gacgctggcc cgagcctgtt
    11461 gttcatcaca tacgccgagg ctatcgcgaa catgcccgca tctactttct tcgccatcat
    11521 ctttttcctg atgctgataa cccttgggct ggatagcacc ttcgccggac tggagggtgt
    11581 catcactgcc gttctcgacg agttccccca tgtgtgggcc aagcgacggg aaaggttcgt
    11641 gctggccgtc gtaatcacat gcttcttcgg ctcactggtg acccttacct ttggcggtgc
    11701 gtacgtggtc aagctgctcg aagagtacgc cactggaccg gcggttctga ccgtggcatt
    11761 gatcgaggcc gtggccgtaa gctggtttta cggcatcacg cagttctgta gggacgtgaa
    11821 agagatgctg ggcttttctc ccggttggtt ttggaggatt tgttgggtag caattagccc
    11881 cctgtttctc cttttcatta tttgctcttt tctgatgtct ccaccgcagc tccgactgtt
    11941 tcagtacaac tacccctact ggtcaattat cctgggctat tgtatcggca cctcaagttt
    12001 catctgcatc cccacataca tcgcatacag actgataatt acgcccggta cttttaagga
    12061 gcgaatcatt aaaagcataa cccccgaaac ccccaccgag atcccttgtg gcgatataag
    12121 actcaatgcg gtctaatagc tcgag
//
