LOCUS       sequence-205624-        2774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mEGFP-linker sequence for N-terminus tagging of 
            human CTNNB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-47: CTNNB1-mEGFP
FEATURES             Location/Qualifiers
     source          1..2774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2261..2296
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ctcaaaaaaa aacaaaacat agttcacact taaatatttt attccatatc tttacatacc
       61 caatatgtta atttatagtt caagatgaac ttgtttggga cagattttgt aataaaggaa
      121 atcgtgttat tagaaatatc tagaggccat gagcccttaa actgttctaa tttgcaagta
      181 gttccctgtg tgatgcagtt tttttcaata ttgcacaata aaggcaaaat acggacaaat
      241 tagatgataa gatttatata aatttttaaa atattgatca aaatatgtat ccatattggt
      301 aatatttgta tttataataa atcattgctg taaatttgaa cttagaaaaa ttttactaat
      361 aaaggtgctt ttgtgttgca aactttcatt tgaaaagtaa tttttctttg taccaaaaaa
      421 tctaaaattc gctattctag tcaccaaaat ttgctttatg aaaaataatt tttgatggca
      481 ctatatcaga aaacaacttg ttaaagaaaa tgtggagttt ttaaaatccc actgtacctc
      541 tgttatccaa aggggatctg tgaatttttc tgtgaaaggt taaaaaagga gagaccttta
      601 ggaattcaga gagcagctga tttttgaata gtgttttccc ctccctggct tttattatta
      661 caactctgtg ctttttcatc accatcctga atatctataa ttaatattta tactattaat
      721 aaaaagacat ttttggtaag gaggagtttt cactgaagtt cagcagtgat ggagctgtgg
      781 ttgaggtgtc tggaggagac catgaggtct gcgtttcact aacctggtaa aagaggatat
      841 gggttttttt tgtgggtgta atagtgacat ttaacaggta tcccagtgac ttaggagtat
      901 taatcaagct aaatttaaat cctaatgact tttgattaac tttttttagg gtatttgaag
      961 tataccatac aactgttttg aaaacccggt gtggacaatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagagcggc ctgagaagca gagcccaggc
     1741 cagcaacagc gccgtggacg gaaccgcagc aaccgctact caaggtttgt gtcattaaat
     1801 ctttagttac tgaattgggg ctctgcttcg ttgccattaa gccagtctgg ctgagatccc
     1861 cctgctttcc tctctccctg cttacttgtc aggctacctt ttgctccatt ttctgctcac
     1921 tcctcctaat ggcttggtga aatagcaaac aagccaccag caggaatcta gtctggatga
     1981 ctgcttctgg agcctggatg cagtaccatt cttccactga ttcagtgagt aactgttagg
     2041 tggttcccta agggattagg tatttcatca ctgagctaac cctggctatc attctgcttt
     2101 tcttggctgt ctttcagatt tgactttatt tctaaaaata tttcaatggg tcatatcaca
     2161 gattcttttt ttttaaatta aagtaacatt tccaatctac taatgctaat actgtttcgt
     2221 atttatagct gatttgatgg agttggacat ggccatggaa ccagacagaa aagcggctgt
     2281 tagtcactgg cagcaacagt cttacctgga ctctggaatc cattctggtg ccactaccac
     2341 agctccttct ctgagtggta aaggcaatcc tgaggaagag gatgtggata cctcccaagt
     2401 cctgtatgag tgggaacagg gattttctca gtccttcact caagaacaag tagctggtaa
     2461 gagtattatt tttcattgcc ttactgaaag tcagaatgca gttttgagaa ctaaaaagtt
     2521 agtgtataat agtttaaata aaatgttgtg gtgaagaaaa gagagtaata gcaatgtcac
     2581 ttttaccatt taggatagca aatacttagg taaatgctga actgtggata gtgagtgttg
     2641 aattaacctt ttccagatat tgatggacag tatgcaatga ctcgagctca gagggtacga
     2701 gctgctatgt tccctgagac attagatgag ggcatgcaga tcccatctac acagtttgat
     2761 gctgctcatc ccac
//
