LOCUS       sequence-205625-        2732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human GJA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-43: GJA1-mEGFP
FEATURES             Location/Qualifiers
     source          1..2732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1019..1021,1022..1735)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1061..1082)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1322..1341)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1669..1690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtctgccttt cgttgtaaca ctcagcaacc tggttgtgaa aatgtctgct atgacaagtc
       61 tttcccaatc tctcatgtgc gcttctgggt cctgcagatc atatttgtgt ctgtacccac
      121 actcttgtac ctggctcatg tgttctatgt gatgcgaaag gaagagaaac tgaacaagaa
      181 agaggaagaa ctcaaggttg cccaaactga tggtgtcaat gtggacatgc acttgaagca
      241 gattgagata aagaagttca agtacggtat tgaagagcat ggtaaggtga aaatgcgagg
      301 ggggttgctg cgaacctaca tcatcagtat cctcttcaag tctatctttg aggtggcctt
      361 cttgctgatc cagtggtaca tctatggatt cagcttgagt gctgtttaca cttgcaaaag
      421 agatccctgc ccacatcagg tggactgttt cctctctcgc cccacggaga aaaccatctt
      481 catcatcttc atgctggtgg tgtccttggt gtccctggcc ttgaatatca ttgaactctt
      541 ctatgttttc ttcaagggcg ttaaggatcg ggttaaggga aagagcgacc cttaccatgc
      601 gaccagtggt gcgctgagcc ctgccaaaga ctgtgggtct caaaaatatg cttatttcaa
      661 tggctgctcc tcaccaaccg ctcccctctc gcctatgtct cctcctgggt acaagctggt
      721 tactggcgac agaaacaatt cttcttgccg caattacaac aagcaagcaa gtgagcaaaa
      781 ctgggctaat tacagtgcag aacaaaatcg aatggggcag gcgggaagca ccatctctaa
      841 ctcccatgca cagccttttg atttccccga tgataaccag aattctaaaa aactagctgc
      901 tggacatgaa ttacagccac tagccattgt ggaccagcga ccttcaagca gagccagcag
      961 tcgtgccagc agcagacctc gcccagacga cctggagatc gaccctcctg tggccacagt
     1021 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1081 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1141 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1201 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1261 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1321 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1381 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1441 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1501 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1561 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1621 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1681 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtagataca
     1741 ggcttgaaag catcaagatt ccactcaatt gtgcagaaga aaaaaggtgc tgtagaaagt
     1801 gcaccaggtg ttaattttga tccggtggag gtggtactca acagccttat tcatgaggct
     1861 tagaaaacac aaagacatta gaatacctag gttcactggg ggtgtatggg gtagatgggt
     1921 ggagagggag gggataagag aggtgcatgt tggtatttaa agtagtggat tcaaagaact
     1981 tagattataa ataagagttc cattaggtga tacatagata agggcttttt ctccccgcaa
     2041 acacccctaa gaatggttct gtgtatgtga atgagcgggt ggtaattgtg gctaaatatt
     2101 tttgttttac caagaaactg aaataattct ggccaggaat aaatacttcc tgaacatctt
     2161 aggtcttttc aacaagaaaa agacagagga ttgtccttaa gtccctgcta aaacattcca
     2221 ttgttaaaat ttgcactttg aaggtaagct ttctaggcct gaccctccag gtgtcaatgg
     2281 acttgtgcta ctatattttt ttattcttgg tatcagttta aaattcagac aaggcccaca
     2341 gaataagatt ttccatgcat ttgcaaatac gtatattctt tttccatcca cttgcacaat
     2401 atcattacca tcactttttc atcattcctc agctactact cacattcatt taatggtttc
     2461 tgtaaacatt tttaagacag ttgggatgtc acttaacatt ttttttttga gctaaagtca
     2521 gggaatcaag ccatgcttaa tatttaacaa tcacttatat gtgtgtcgaa gagtttgttt
     2581 tgtttgtcat gtattggtac aagcagatac agtataaact cacaaacaca gatttgaaaa
     2641 taatgcacat atggtgttca aatttgaacc tttctcatgg atttttgtgg tgtgggccaa
     2701 tatggtgttt acattatata attcctgctg tg
//
