LOCUS       sequence-205626-        2732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human HIST1H2BJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-52: HIST1H2BJ-mEGFP
FEATURES             Location/Qualifiers
     source          1..2732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             623..1000
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     CDS             join(1019..1021,1022..1735)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1061..1082)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1322..1341)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1669..1690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctttgcccag cagcttgttg agctcctcgt cgttgcggat ggccagttgg agatgacgcg
       61 ggatgatgcg ggtcttcttg ttgtcgcggg ccgcgttgcc cgccagttcc aggatctcgg
      121 cggtcaggta ctccagcacc gctgccagat acaccggcgc gccggccccg acccgctcgg
      181 catagttgcc tttgcggagc aggcggtgca ctcggcccac ggggaactgg agaccggccc
      241 tagaagagcg agtcttggcc ttagcgcggg ctttgcctcc ctgcttgcca cgtccagaca
      301 tagcgagcgc aactcactac gagcaaccac aaagtgaacg ggaaaggcgg cgctttttat
      361 aaacactatt gggcgcgaaa aagaagacgt gttgttggtt agggctgcag tttaatttca
      421 accaatagta gtgcgtcttc tggatttgcg aatcctgatt gggcagacct gacctctgac
      481 gttaccctga ataactacca atcagacaca agacttcaac tcttcacctt atttgcataa
      541 gcgattctat ataaaagcgc cttgtcatac cctgctcacg ctgtttttcc ttttcgttgg
      601 cgctttatag ctacacagtg ctatgccaga gccagcgaag tctgctcccg ccccgaaaaa
      661 gggctccaag aaggcggtga ctaaggcgca gaagaaagac ggcaagaagc gcaagcgcag
      721 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
      781 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
      841 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag
      901 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc
      961 cgagggtact aaggccgtca ccaagtacac cagcgctaag gaccctcctg tggccaccgt
     1021 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1081 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1141 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1201 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1261 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1321 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1381 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1441 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1501 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1561 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1621 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1681 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaacagt
     1741 gagttggttg caaactctca atcctaacgc ctcttttaag agccacccat gttctcaaag
     1801 aaagagctgg tgcttgtatt cctcctctgc tggccactga caaacccttg taacttgcta
     1861 ctgtgttttt tggtctgaag tagagcagtt atttaactaa tccttagtga cttttttttt
     1921 ttagatctgc cattctaatc ttagagttaa gtaaggagat gggaaatttt ctattataag
     1981 ttcgaaacca attaaaatac gttagaaacc aattaaaata ctcgtcggtc ccccgtcggt
     2041 tagtgatttg gaacagtgcc aagttgcagc ggttgtcagt ttgaatttgc ccgggcaacg
     2101 cccgcccttc ctctgcatcc tgattggttg ttgtatccag gaagtccacg gtcggcttgg
     2161 gtgcgccaaa ggaatccaaa ccccgccttt atttggcctt ccttacaaac tcagtaaact
     2221 gtcagtttct cattctttag tattttatcg ctttcgggaa ggagatttat ttctaattag
     2281 ttttcgttgg atagagcgtt ttttttttta aagggaccct ttatggcaac ataatcagtt
     2341 tttttttttt tgagaaggag acttcctctg tcgcccagac tggagtgcgg tggcgcgatc
     2401 tcagctcact gcagcctccg cctcccgggt tccagcgatt ctcctgcttc acccttccgt
     2461 gtagctggga ttacaggcat gcgccaccat gcccggataa tttttgtatt tttagtagag
     2521 atggggtttc accatgttgg ctaggctggt ctcgaactga cttcaggtga tccactagcc
     2581 tcagccttaa agtgctagga ttacaggcat gagccatcaa gcccggctac aacggaattt
     2641 gaatgctgta gttttcagcc tgagaaagat caatgcttta tttctgatta gaatactatt
     2701 ttccctttat ggtatatcta tataagcaaa gg
//
