LOCUS       sequence-205628-       11445 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation A173G with approximately
            wildtype activity, decreased surface expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTA173G-myc
FEATURES             Location/Qualifiers
     source          1..11445
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(690..708)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        776..880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(881..949,950..1741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1099..1118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1912..2500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2401..2420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2931..3076
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3119..4156
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4154..4173)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4198..4246
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4900..5279
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5280..5483
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5434..5454
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5480..5504
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5532..5541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6207..6236
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7525..7659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7549..7568)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7603..7622
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7802..7824)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8384..10173
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg
       61 tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc
      121 tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga
      181 gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg
      241 cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc
      301 tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc
      361 cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct
      421 cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg
      481 cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg
      541 atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg
      601 gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt
      661 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      721 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
      781 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      841 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      901 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      961 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1021 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1081 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     1141 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1201 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1261 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1321 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     1381 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     1441 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     1501 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     1561 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     1621 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     1681 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1741 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1801 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1861 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1921 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1981 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2041 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2101 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2161 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2221 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2281 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2341 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2401 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2461 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2521 tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg
     2581 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     2641 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     2701 ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc
     2761 caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga
     2821 gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt
     2881 tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg atgacacaaa
     2941 ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg
     3001 gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct
     3061 gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc aatgagatat
     3121 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     3181 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     3241 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     3301 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     3361 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     3421 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     3481 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     3541 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     3601 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     3661 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     3721 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     3781 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     3841 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     3901 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     3961 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     4021 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     4081 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg atcgggagat
     4141 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     4201 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt
     4261 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     4321 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     4381 gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta gggacggggt
     4441 cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt gcgtggggtc
     4501 aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg ggcatggacc
     4561 gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc cccgaccccc
     4621 aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc atgtttgaca
     4681 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     4741 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     4801 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     4861 acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta ttgactagtt
     4921 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     4981 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     5041 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     5101 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
     5161 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     5221 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     5281 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
     5341 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     5401 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
     5461 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatctctaga agctgggtac
     5521 cagctgctag cgccaccatg gagaccaccc ccctgaacag tcaaaagcaa ctctctgcct
     5581 gtgaggatgg agaggactgt caggagaatg gcgttcttca aaaggttgta cccacccctg
     5641 gagataaggt ggaaagcggc cagatttcca acggctacag cgccgtgcca tcacctggag
     5701 ccggggatga tacacggcac agcattcccg caaccaccac gacactggtt gcagagcttc
     5761 accagggcga gagggagacg tggggcaaaa aggtagattt tctgctttcc gtgatcggtt
     5821 acgcggtcga tctggggaac gtgtggaggt tcccttacat ttgttaccaa aacggagggg
     5881 gtgccttctt gctgccctac acaattatgg cgatttttgg gggtatcccg ctcttctaca
     5941 tggaactggc cttgggtcag taccaccgga acggctgcat tagcatttgg cgcaagatat
     6001 gccccatttt caagggaatc ggatacgcaa tctgtataat cgcgttctat attggctcat
     6061 attacaacac catcatggcc tgggcccttt attacttgat cagcagtttc accgaccaat
     6121 tgccgtggac gagctgtaag aactcatgga ataccgggaa ctgtaccaac tacttctctg
     6181 aggacagctc aggaggtagc gggggtgaac agaagctgat tagcgaggag gacttgggca
     6241 gtccaggcgg aagcggcaac atcacttgga cactccatag tacgagcccc gcagaggagt
     6301 tttacaccag gcacgtactg cagattcata ggagcaaggg tctgcaagac ctgggcggta
     6361 ttagctggca gctcgcattg tgcataatgt tgatctttac ggtgatctat ttctctatct
     6421 ggaagggagt aaagacatcc ggcaaggtgg tgtgggtgac ggcgaccttc ccctacatta
     6481 tactcagcgt gttgttggtg aggggggcaa ccctgcctgg ggcctggcga ggcgtgctgt
     6541 tctatctcaa gccaaactgg cagaagcttc ttgaaaccgg agtgtggatc gacgcggccg
     6601 cgcaaatctt cttttccctc ggtcccggct tcggggtcct tctggcattc gcaagctaca
     6661 acaagttcaa caataactgc taccaggacg ccctcgtgac aagtgtagtg aactgcatga
     6721 cctctttcgt gagcgggttt gtgatcttca cagtactcgg atatatggct gagatgagga
     6781 acgaagatgt tagtgaggtt gccaaagacg ctggcccgag cctgttgttc atcacatacg
     6841 ccgaggctat cgcgaacatg cccgcatcta ctttcttcgc catcatcttt ttcctgatgc
     6901 tgataaccct tgggctggat agcaccttcg ccggactgga gggtgtcatc actgccgttc
     6961 tcgacgagtt cccccatgtg tgggccaagc gacgggaaag gttcgtgctg gccgtcgtaa
     7021 tcacatgctt cttcggctca ctggtgaccc ttacctttgg cggtgcgtac gtggtcaagc
     7081 tgctcgaaga gtacgccact ggaccggcgg ttctgaccgt ggcattgatc gaggccgtgg
     7141 ccgtaagctg gttttacggc atcacgcagt tctgtaggga cgtgaaagag atgctgggct
     7201 tttctcccgg ttggttttgg aggatttgtt gggtagcaat tagccccctg tttctccttt
     7261 tcattatttg ctcttttctg atgtctccac cgcagctccg actgtttcag tacaactacc
     7321 cctactggtc aattatcctg ggctattgta tcggcacctc aagtttcatc tgcatcccca
     7381 catacatcgc atacagactg ataattacgc ccggtacttt taaggagcga atcattaaaa
     7441 gcataacccc cgaaaccccc accgagatcc cttgtggcga tataagactc aatgcggtct
     7501 aatagctcga ggccggcaag gccggatcca gacatgataa gatacattga tgagtttgga
     7561 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     7621 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     7681 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     7741 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     7801 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7861 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     7921 ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     7981 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     8041 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     8101 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     8161 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     8221 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     8281 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     8341 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     8401 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     8461 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     8521 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     8581 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     8641 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     8701 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     8761 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     8821 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     8881 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     8941 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     9001 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     9061 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     9121 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     9181 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     9241 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     9301 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     9361 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     9421 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     9481 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     9541 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     9601 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     9661 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     9721 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     9781 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     9841 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     9901 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     9961 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
    10021 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca
    10081 tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg
    10141 gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct taggtgaatt
    10201 taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat
    10261 gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac atgggtatgc
    10321 ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca gaaatacacc
    10381 aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat acacggcttt
    10441 taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc tcaccctcat
    10501 ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca gccccttcca
    10561 ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc
    10621 tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca tccttcaaaa
    10681 cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg gactccctta
    10741 gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga ctcaatggtg
    10801 taagacgaca ttgtggaata gcaagggcag ttcctcgcct taggttgtaa agggaggtct
    10861 tactacctcc atatacgaac acaccggcga cccaagttcc ttcgtcggta gtcctttcta
    10921 cgtgactcct agccaggaga gctcttaaac cttctgcaat gttctcaaat ttcgggttgg
    10981 aacctccttg accacgatgc tttccaaacc accctccttt tttgcgcctg cctccatcac
    11041 cctgaccccg gggtccagtg cttgggcctt ctcctgggtc atctgcgggg ccctgctcta
    11101 tcgctcccgg gggcacgtca ggctcaccat ctgggccacc ttcttggtgg tattcaaaat
    11161 aatcggcttc ccctacaggg tggaaaaatg gccttctacc tggagggggc ctgcgcggtg
    11221 gagacccgga tgatgatgac tgactactgg gactcctggg cctcttttct ccacgtccac
    11281 gacctctccc cctggctctt tcacgacttc cccccctggc tctttcacgt cctctacccc
    11341 ggcggcctcc actacctcct cgaccccggc ctccactacc tcctcgaccc cggcctccac
    11401 tgcctcctcg accccggcct ccacctcctg ctcctgcccc tcctg
//
