LOCUS       sequence-205630-       12071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses IGF1R, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 12071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) IGF1R
FEATURES             Location/Qualifiers
     source          1..12071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      56..644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     545..564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     798..815
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    932..953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(968..973,974..991,992..998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1006..1022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1011..1033
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1030..1046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1030..1046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1065..1085
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1067..1085
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1113..1339
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1340..1520
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1567..1692
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2185..2418
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2603..2647
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2914..3030
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3053..3356
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3357..3560
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3511..3531
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3557..3581
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3665..3689
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7800..7824)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7855..8443
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7908..7928)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8326..8337)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8457..8944
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8492..8515)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8877..8896
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8965..9564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8965..8984)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9461..9481
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9702..9935
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10007..10128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10044..10063)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10098..10117
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10168..10303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10323..10342)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10324..10342)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10352..10369)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10352..10368)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10361..10383)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10510..10965
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10597..10616)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10807..10828
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10991..11095
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11096..11164,11165..11956)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11314..11333)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
       61 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      841 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
      901 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      961 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     1021 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     1081 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     1141 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     1201 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     1261 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     1321 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     1381 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     1441 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     1501 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     1561 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     1621 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     1681 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     1741 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     1801 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     1861 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     1921 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     1981 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     2041 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     2101 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     2161 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     2221 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     2281 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     2341 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     2401 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     2461 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     2521 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     2581 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     2641 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     2701 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2761 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2821 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2881 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     2941 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     3001 acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc cgcgttacat
     3061 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     3121 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     3181 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     3241 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     3301 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     3361 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     3421 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     3481 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     3541 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     3601 gctgttttga cctccataga agacaccgac tctagtccag tgtggtggaa ttctgcagat
     3661 atcaacaagt ttgtacaaaa aagcaggctt caccatgaag tctggctccg gaggagggtc
     3721 cccgacctcg ctgtgggggc tcctgtttct ctccgccgcg ctctcgctct ggccgacgag
     3781 tggagaaatc tgcgggccag gcatcgacat ccgcaacgac tatcagcagc tgaagcgcct
     3841 ggagaactgc acggtgatcg agggctacct ccacatcctg ctcatctcca aggccgagga
     3901 ctaccgcagc taccgcttcc ccaagctcac ggtcattacc gagtacttgc tgctgttccg
     3961 agtggctggc ctcgagagcc tcggagacct cttccccaac ctcacggtca tccgcggctg
     4021 gaaactcttc tacaactacg ccctggtcat cttcgagatg accaatctca aggatattgg
     4081 gctttacaac ctgaggaaca ttactcgggg ggccatcagg attgagaaaa atgctgacct
     4141 ctgttacctc tccactgtgg actggtccct gatcctggat gcggtgtcca ataactacat
     4201 tgtggggaat aagcccccaa aggaatgtgg ggacctgtgt ccagggacca tggaggagaa
     4261 gccgatgtgt gagaagacca ccatcaacaa tgagtacaac taccgctgct ggaccacaaa
     4321 ccgctgccag aaaatgtgcc caagcacgtg tgggaagcgg gcgtgcaccg agaacaatga
     4381 gtgctgccac cccgagtgcc tgggcagctg cagcgcgcct gacaacgaca cggcctgtgt
     4441 agcttgccgc cactactact atgccggtgt ctgtgtgcct gcctgcccgc ccaacaccta
     4501 caggtttgag ggctggcgct gtgtggaccg tgacttctgc gccaacatcc tcagcgccga
     4561 gagcagcgac tccgaggggt ttgtgatcca cgacggcgag tgcatgcagg agtgcccctc
     4621 gggcttcatc cgcaacggca gccagagcat gtactgcatc ccttgtgaag gtccttgccc
     4681 gaaggtctgt gaggaagaaa agaaaacaaa gaccattgat tctgttactt ctgctcagat
     4741 gctccaagga tgcaccatct tcaagggcaa tttgctcatt aacatccgac gggggaataa
     4801 cattgcttca gagctggaga acttcatggg gctcatcgag gtggtgacgg gctacgtgaa
     4861 gatccgccat tctcatgcct tggtctcctt gtccttccta aaaaaccttc gcctcatcct
     4921 aggagaggag cagctagaag ggaattactc cttctacgtc ctcgacaacc agaacttgca
     4981 gcaactgtgg gactgggacc accgcaacct gaccatcaaa gcagggaaaa tgtactttgc
     5041 tttcaatccc aaattatgtg tttccgaaat ttaccgcatg gaggaagtga cggggactaa
     5101 agggcgccaa agcaaagggg acataaacac caggaacaac ggggagagag cctcctgtga
     5161 aagtgacgtc ctgcatttca cctccaccac cacgtcgaag aatcgcatca tcataacctg
     5221 gcaccggtac cggccccctg actacaggga tctcatcagc ttcaccgttt actacaagga
     5281 agcacccttt aagaatgtca cagagtatga tgggcaggat gcctgcggct ccaacagctg
     5341 gaacatggtg gacgtggacc tcccgcccaa caaggacgtg gagcccggca tcttactaca
     5401 tgggctgaag ccctggactc agtacgccgt ttacgtcaag gctgtgaccc tcaccatggt
     5461 ggagaacgac catatccgtg gggccaagag tgagatcttg tacattcgca ccaatgcttc
     5521 agttccttcc attcccttgg acgttctttc agcatcgaac tcctcttctc agttaatcgt
     5581 gaagtggaac cctccctctc tgcccaacgg caacctgagt tactacattg tgcgctggca
     5641 gcggcagcct caggacggct acctttaccg gcacaattac tgctccaaag acaaaatccc
     5701 catcaggaag tatgccgacg gcaccatcga cattgaggag gtcacagaga accccaagac
     5761 tgaggtgtgt ggtggggaga aagggccttg ctgcgcctgc cccaaaactg aagccgagaa
     5821 gcaggccgag aaggaggagg ctgaataccg caaagtcttt gagaatttcc tgcacaactc
     5881 catcttcgtg cccagacctg aaaggaagcg gagagatgtc atgcaagtgg ccaacaccac
     5941 catgtccagc cgaagcagga acaccacggc cgcagacacc tacaacatca ctgacccgga
     6001 agagctggag acagagtacc ctttctttga gagcagagtg gataacaagg agagaactgt
     6061 catttctaac cttcggcctt tcacattgta ccgcatcgat atccacagct gcaaccacga
     6121 ggctgagaag ctgggctgca gcgcctccaa cttcgtcttt gcaaggacta tgcccgcaga
     6181 aggagcagat gacattcctg ggccagtgac ctgggagcca aggcctgaaa actccatctt
     6241 tttaaagtgg ccggaacctg agaatcccaa tggattgatt ctaatgtatg aaataaaata
     6301 cggatcacaa gttgaggatc agcgagaatg tgtgtccaga caggaataca ggaagtatgg
     6361 aggggccaag ctaaaccggc taaacccggg gaactacaca gcccggattc aggccacatc
     6421 tctctctggg aatgggtcgt ggacagatcc tgtgttcttc tatgtccagg ccaaaacagg
     6481 atatgaaaac ttcatccatc tgatcatcgc tctgcccgtc gctgtcctgt tgatcgtggg
     6541 agggttggtg attatgctgt acgtcttcca tagaaagaga aataacagca ggctggggaa
     6601 tggagtgctg tatgcctctg tgaacccgga gtacttcagc gctgctgatg tgtacgttcc
     6661 tgatgagtgg gaggtggctc gggagaagat caccatgagc cgggaacttg ggcaggggtc
     6721 gtttgggatg gtctatgaag gagttgccaa gggtgtggtg aaagatgaac ctgaaaccag
     6781 agtggccatt aaaacagtga acgaggccgc aagcatgcgt gagaggattg agtttctcaa
     6841 cgaagcttct gtgatgaagg agttcaattg tcaccatgtg gtgcgattgc tgggtgtggt
     6901 gtcccaaggc cagccaacac tggtcatcat ggaactgatg acacggggcg atctcaaaag
     6961 ttatctccgg tctctgaggc cagaaatgga gaataatcca gtcctagcac ctccaagcct
     7021 gagcaagatg attcagatgg ccggagagat tgcagacggc atggcatacc tcaacgccaa
     7081 taagttcgtc cacagagacc ttgctgcccg gaattgcatg gtagccgaag atttcacagt
     7141 caaaatcgga gattttggta tgacgcgaga tatctatgag acagactatt accggaaagg
     7201 agggaaaggg ctgctgcccg tgcgctggat gtctcctgag tccctcaagg atggagtctt
     7261 caccacttac tcggacgtct ggtccttcgg ggtcgtcctc tgggagatcg ccacactggc
     7321 cgagcagccc taccagggct tgtccaacga gcaagtcctt cgcttcgtca tggagggcgg
     7381 ccttctggac aagccagaca actgtcctga catgctgttt gaactgatgc gcatgtgctg
     7441 gcagtataac cccaagatga ggccttcctt cctggagatc atcagcagca tcaaagagga
     7501 gatggagcct ggcttccggg aggtctcctt ctactacagc gaggagaaca agctgcccga
     7561 gccggaggag ctggacctgg agccagagaa catggagagc gtccccctgg acccctcggc
     7621 ctcctcgtcc tccctgccac tgcccgacag acactcagga cacaaggccg agaacggccc
     7681 cggccctggg gtgctggtcc tccgcgccag cttcgacgag agacagcctt acgcccacat
     7741 gaacgggggc cgcaagaacg agcgggcctt gccgctgccc cagtcttcga cctgctgaaa
     7801 cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgacaatcaa
     7861 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7921 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7981 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8041 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8101 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8161 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8221 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     8281 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8341 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8401 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctaccgggt
     8461 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     8521 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     8581 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     8641 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     8701 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     8761 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     8821 gtccgggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     8881 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     8941 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     9001 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     9061 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     9121 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     9181 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     9241 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     9301 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     9361 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     9421 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     9481 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     9541 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     9601 agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc
     9661 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc
     9721 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9901 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     9961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
    10021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
    10141 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    10201 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    10261 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
    10321 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
    10381 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
    10441 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
    10501 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    10561 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    10621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    10681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    10741 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    10801 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    10861 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    10921 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
    10981 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    11041 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    11101 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    11161 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    11221 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    11281 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    11341 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    11401 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    11461 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    11521 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    11581 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    11641 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    11701 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    11761 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    11821 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    11881 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    11941 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    12001 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    12061 aatcccttaa c
//
