LOCUS       sequence-205633-        8594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MXRA7, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 8594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) MXRA7
FEATURES             Location/Qualifiers
     source          1..8594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      41..629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     530..549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     783..800
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    917..938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(953..958,959..976,977..983)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    991..1007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     996..1018
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1015..1031
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1015..1031
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1050..1070
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1052..1070
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1098..1324
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1325..1505
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1552..1677
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2170..2403
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2588..2632
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2899..3015
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3038..3341
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3342..3545
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3496..3516
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3542..3566
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3650..3674
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4296..4320)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4351..4939
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4404..4424)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4822..4833)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4953..5452
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4988..5011)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5385..5404
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5473..6072
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5473..5492)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5969..5989
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6210..6443
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6515..6636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6552..6571)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6606..6625
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6676..6811
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6831..6850)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6832..6850)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6860..6877)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6860..6876)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6869..6891)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7018..7473
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7105..7124)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7315..7336
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7499..7603
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7604..7672,7673..8464)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7822..7841)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
       61 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      181 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      661 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      721 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      781 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      841 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      901 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      961 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     1021 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     1081 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     1141 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     1201 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     1261 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     1321 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     1381 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     1441 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     1501 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     1561 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     1621 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     1681 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     1741 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     1801 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1861 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1921 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1981 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     2041 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     2101 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     2161 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     2221 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     2281 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     2341 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     2401 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     2461 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     2521 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     2581 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     2641 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     2701 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     2761 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2821 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2881 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2941 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     3001 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
     3061 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     3121 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     3181 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     3241 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     3301 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     3361 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     3421 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     3481 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     3541 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     3601 atagaagaca ccgactctag tccagtgtgg tggaattctg cagatatcaa caagtttgta
     3661 caaaaaagca ggcttcacca tggaagcacc cgcagaacta ttagcagctt taccagctct
     3721 tgctactgcc ctcgcactcc tgcttgcttg gttgctggtg cgtagaggag ccgctgcgag
     3781 tcctgaacca gctagagcac cacccgagcc ggcaccgcca gcagaggcta caggagcgcc
     3841 tgcaccttcc agaccttgcg ctcccgaacc cgccgccagc ccagctggcc cagaagaacc
     3901 aggggaacct gcgggtttag gagagctggg tgagcctgcc ggtccaggcg aacccgaagg
     3961 ccccggggat cctgctgccg cccctgcaga ggccgaagaa caggccgtcg aggcccggca
     4021 agaggaggaa caggacctgg atggagagaa aggccccagc tcagaagggc ccgaggaaga
     4081 agatggcgag gggttttcct ttaaatactc tcccggaaag ctccgaggca atcagtacaa
     4141 gaaaatgatg acgaaagagg agctcgagga ggagcagagg gtgcaaaagg agcagctggc
     4201 cgccatcttc aagctgatga aggataacaa ggagaccttc ggagagatgt ctgacgggga
     4261 cgttcaggag cagctgaggc tgtatgacat gtgaaaccca gctttcttgt acaaagtggt
     4321 tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa
     4381 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4441 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4501 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4561 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4621 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4681 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4741 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4801 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4861 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4921 ctttgggccg cctccccgcc tggaattcta ccgggtaggg gaggcgcttt tcccaaggca
     4981 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     5041 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     5101 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     5161 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     5221 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     5281 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     5341 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     5401 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     5461 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5521 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5581 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5641 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5701 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5761 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5821 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5881 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5941 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6001 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6061 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     6121 catgcatctc gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct
     6181 tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca
     6241 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6301 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6361 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6421 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6481 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     6541 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6601 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     6661 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6721 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6781 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     6841 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6901 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6961 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     7021 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7081 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7141 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7201 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7261 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7321 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7381 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7441 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     7501 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7561 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7621 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7681 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7741 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7801 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7861 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7921 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7981 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8041 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8101 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8161 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8221 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8281 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8341 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8401 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8461 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8521 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8581 tgagttttcg ttcc
//
