LOCUS       sequence-205634-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MXRA7, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) MXRA7
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(598..622)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(706..726)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tattagcagc tttaccagct cttgctactg ccctcgcact cctgcttgct tggttgctgg
       61 tgcgtagagg agccgctgcg agtcctgaac cagctagagc accacccgag ccggcaccgc
      121 cagcagaggc tacaggagcg cctgcacctt ccagaccttg cgctcccgaa cccgccgcca
      181 gcccagctgg cccagaagaa ccaggggaac ctgcgggttt aggagagctg ggtgagcctg
      241 ccggtccagg cgaacccgaa ggccccgggg atcctgctgc cgcccctgca gaggccgaag
      301 aacaggccgt cgaggcccgg caagaggagg aacaggacct ggatggagag aaaggcccca
      361 gctcagaagg gcccgaggaa gaagatggcg aggggttttc ctttaaatac tctcccggaa
      421 agctccgagg caatcagtac aagaaaatga tgacgaaaga ggagctcgag gaggagcaga
      481 gggtgcaaaa ggagcagctg gccgccatct tcaagctgat gaaggataac aaggagacct
      541 tcggagagat gtctgacggg gacgttcagg agcagctgag gctgtatgac atgtgaaacc
      601 cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg acaatcaacc
      661 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
      721 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
      781 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
      841 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
      901 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
      961 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tg
//
