LOCUS       sequence-205636-        9308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SDC3, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) SDC3
FEATURES             Location/Qualifiers
     source          1..9308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      34..622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     523..542
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     776..793
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    910..931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(946..951,952..969,970..976)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    984..1000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     989..1011
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1008..1024
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1008..1024
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1043..1063
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1045..1063
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1091..1317
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1318..1498
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1545..1670
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2163..2396
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2581..2625
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2892..3008
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3031..3334
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3335..3538
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3489..3509
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3535..3559
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3643..3667
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5003..5027)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5058..5646
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5111..5131)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5529..5540)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5660..6159
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5695..5718)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6092..6111
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6180..6779
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6180..6199)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6676..6696
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6917..7150
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7222..7343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7259..7278)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7313..7332
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7383..7518
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7538..7557)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7539..7557)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7567..7584)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7567..7583)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7576..7598)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7725..8180
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7812..7831)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8022..8043
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8206..8310
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8311..8379,8380..9171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8529..8548)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
       61 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      121 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
      181 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      241 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      301 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      361 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      421 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      481 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      541 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      601 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      661 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      721 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      781 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      841 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      901 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      961 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     1021 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     1081 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     1141 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     1201 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     1261 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     1321 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1381 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1441 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1501 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1561 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1621 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1681 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1741 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1801 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1861 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1921 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1981 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2041 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2101 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2161 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2221 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2281 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2341 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2401 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2461 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2521 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2581 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2641 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2701 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2761 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2821 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     2881 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2941 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc
     3001 aaaattttat cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc
     3061 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     3121 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     3181 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3241 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3301 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3361 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3421 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3481 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     3541 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     3601 acaccgactc tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
     3661 gcaggcttca ccatgaaacc aggtcctccc catcgagcgg gagcagcgca cggcgcggga
     3721 gccggggcag gtgctgcagc tgggcccggt gcaagggggt tactcctccc tccactcctt
     3781 ctattacttc ttgcgggaag agctgcaggt gctcagagat ggagatctga gaacttcgag
     3841 agacccgtag atttagaggg tagtggtgac gacgatagct ttcccgatga tgagcttgat
     3901 gatttgtatt ccgggagcgg gagcgggtac ttcgaacagg aaagcggcat agagacggct
     3961 atgcggttta gtcccgacgt cgcgctagcg gtgtccacaa ctcctgcagt tctaccgact
     4021 accaatatac agcctgtcgg gacgcctttc gaagaactgc cgtcggaaag acccactttg
     4081 gagccagcaa catctcccct tgtggtaacg gaggtgccag aggagccatc acaacgggcc
     4141 accacagtga gcacaacgat ggcaacgacc gcagcaacct ctaccgggga tccaacagta
     4201 gcgactgtcc cagccacggt ggctacggca acaccctcta cacccgccgc ccctccgttc
     4261 acagcaacta ccgccgttat tcgtaccaca ggagtccggc gactgctgcc actgcccctc
     4321 acaaccgtgg caacagcccg tgctactacc cccgaagctc cttccccgcc tactactgct
     4381 gccgtcttgg acaccgaggc cccaacacct aggcttgtat ccacagccac tagcaggcca
     4441 agggccctcc caaggcctgc tacaacacag gagcctgaca ttcctgagcg ctcaactctg
     4501 cccttgggca caactgctcc gggacccact gaggttgccc aaacaccaac ccctgaaact
     4561 ttcctgacca ccatccgcga tgagcccgag gtgcctgtgt ctggaggccc ttcaggagat
     4621 tttgagttgc cggaggagga aaccacccag cctgacaccg ccaacgaggt agtggccgtt
     4681 ggaggcgctg ccgctaaagc ttccagtcca cctggaaccc tcccaaaggg cgcccgcccc
     4741 ggcccaggcc tgctggacaa tgccatcgac tctggcagct cggctgctca actgccacag
     4801 aagagtattc tcgaacgcaa ggaggtgctg gtcgccgtca tcgtgggcgg cgtggttggc
     4861 gccctgttcg ccgcctttct ggtgaccctg ctgatctacc ggatgaagaa gaaagacgag
     4921 ggctcgtata ccctggaaga acctaaacaa gcctcagtta cctaccagaa gccagacaaa
     4981 caggaagaat tttatgcctg aaacccagct ttcttgtaca aagtggttga tatccagcac
     5041 agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5101 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5161 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5221 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5281 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5341 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5401 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5461 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5521 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5581 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5641 ccccgcctgg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     5701 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     5761 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     5821 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     5881 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     5941 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     6001 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     6061 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     6121 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     6181 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     6241 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     6301 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6361 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6421 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6481 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6541 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6601 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6661 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6721 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     6781 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag
     6841 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6901 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt
     6961 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7021 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7081 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7141 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7201 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7261 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7321 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7381 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7441 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7501 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     7561 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7621 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7681 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     7741 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7801 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7861 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7921 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     7981 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8041 tggaacaaca ctcaacccta tctcggtcta ttcttttggt ttataaggga ttttgccgat
     8101 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     8161 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8221 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8281 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8341 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8401 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8461 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8521 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8581 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8641 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8701 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8761 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8821 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8881 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8941 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9001 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9061 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9121 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9181 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9241 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9301 cactgagc
//
