LOCUS       sequence-205641-        8699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TSPAN9, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 8699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) TSPAN9
FEATURES             Location/Qualifiers
     source          1..8699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     525..544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     778..795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    912..933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(948..953,954..971,972..978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    986..1002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     991..1013
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1010..1026
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1010..1026
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1045..1065
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1047..1065
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1093..1319
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1320..1500
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1547..1672
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2165..2398
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2583..2627
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2894..3010
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3033..3336
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3337..3540
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3491..3511
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3537..3561
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3645..3669
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4396..4420)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4451..5039
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4504..4524)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4922..4933)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5053..5552
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5088..5111)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5485..5504
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5573..6172
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5573..5592)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6069..6089
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6310..6543
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6615..6736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6652..6671)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6706..6725
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6776..6911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6931..6950)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6932..6950)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6960..6977)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6960..6976)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6969..6991)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7118..7573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7205..7224)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7415..7436
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7599..7703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7704..7772,7773..8564)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7922..7941)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      181 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      241 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      301 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      361 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      421 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      481 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      541 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      601 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      661 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      721 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      781 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      841 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      901 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      961 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     1021 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     1081 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     1141 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     1201 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     1261 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     1321 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1381 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1441 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1501 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1561 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1621 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1681 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1741 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1801 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1861 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1921 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1981 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     2041 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     2101 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     2161 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     2221 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     2281 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     2341 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     2401 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     2461 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2521 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2581 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2641 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2701 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2761 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2821 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     2881 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2941 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat
     3001 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
     3061 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     3121 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     3181 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     3241 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     3301 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     3361 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     3421 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     3481 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     3541 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     3601 agacaccgac tctagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
     3661 aagcaggctt caccatggcc aggggctgcc tctgctgctt gaagtacatg atgttcctct
     3721 tcaatttgat attctggctc tgtggctgtg ggctgctggg agtgggcatc tggctctccg
     3781 tgtcccaagg caactttgcc accttctccc ccagcttccc ttcgttgtct gcagccaacc
     3841 tggtcattgc cataggcacc attgtcatgg tgacgggctt cctcggctgc ctgggggcca
     3901 tcaaggaaaa caagtgcctc ctcctcagct ttttcatcgt cctgttggtc atcctcctag
     3961 cagagctgat cttactcatc ctcttctttg tctacatgga caaggtgaac gagaacgcca
     4021 agaaggacct gaaggaaggc ctgctgctgt accacaccga gaacaacgtg gggctgaaga
     4081 acgcctggaa catcatccag gctgagatgc gatgctgtgg tgtcactgac tacacagact
     4141 ggtacccagt gctgggggag aacacggttc ccgaccgctg ctgcatggag aactcccagg
     4201 gctgcgggcg caacgccacc acgcctttgt ggagaacggg ctgctatgaa aaggtgaaga
     4261 tgtggttcga tgacaataag cacgtgctgg gcacggtggg gatgtgcatc ctcatcatgc
     4321 agatcctggg catggccttc tccatgaccc tcttccagca catccaccgg actggtaaga
     4381 agtacgacgc atgaaaccca gctttcttgt acaaagtggt tgatatccag cacagtggcg
     4441 gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4501 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4561 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4621 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4681 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4741 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4801 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4861 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4921 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4981 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     5041 tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     5101 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     5161 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     5221 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     5281 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     5341 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     5401 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     5461 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     5521 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     5581 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5641 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5701 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5761 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5821 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5881 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5941 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6001 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6061 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6121 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     6181 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg
     6241 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6301 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta
     6361 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6421 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6481 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6541 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6601 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6661 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6721 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6781 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6841 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6901 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     6961 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7021 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7081 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7141 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7201 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7261 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7321 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7381 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7441 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7501 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7561 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7621 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7681 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7741 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7801 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7861 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7921 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7981 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8041 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8101 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8161 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8221 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8281 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8341 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8401 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8461 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8521 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8581 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8641 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactga
//
