LOCUS       sequence-205644-       12113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PHLDB1, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 12113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) PHLDB1
FEATURES             Location/Qualifiers
     source          1..12113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      40..628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     529..548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     782..799
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    916..937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(952..957,958..975,976..982)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    990..1006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     995..1017
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1014..1030
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1014..1030
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1049..1069
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1051..1069
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1097..1323
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1324..1504
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1551..1676
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2169..2402
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2587..2631
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2898..3014
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3037..3340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3341..3544
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3495..3515
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3541..3565
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3649..3673
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7814..7838)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7869..8457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7922..7942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8340..8351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8471..8970
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8506..8529)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8903..8922
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8991..9590
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8991..9010)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9487..9507
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9728..9961
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10033..10154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10070..10089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10124..10143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10194..10329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10349..10368)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10350..10368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10378..10395)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10378..10394)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10387..10409)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10536..10991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10623..10642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10833..10854
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11017..11121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11122..11190,11191..11982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11340..11359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
       61 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      121 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      181 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      241 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      301 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      361 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
      421 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
      481 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
      541 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      601 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
      661 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
      721 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
      781 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
      841 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
      901 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
      961 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1021 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     1081 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     1141 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     1201 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     1261 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     1321 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     1381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     1441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1501 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1561 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1621 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1681 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1741 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1801 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1861 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1921 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1981 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     2041 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     2101 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     2161 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     2221 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     2281 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     2341 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     2401 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     2461 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     2521 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     2581 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2641 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2701 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2761 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2821 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     2881 cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2941 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     3001 aaattcaaaa ttttatcgat aagcttggga gttccgcgtt acataactta cggtaaatgg
     3061 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3121 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     3181 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3241 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3301 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     3361 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     3421 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     3481 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     3541 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     3601 tagaagacac cgactctagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac
     3661 aaaaaagcag gcttcaccat ggatgccttg aatcgaaatc agatagggcc ggggtgccag
     3721 actcagacca tggttcagaa ggggccccta gatttaattg aaaccggtaa agggctcaag
     3781 gtgcaaacgg ataagcctca tttagtttca cttggatcag gccgcctctc aaccgccatc
     3841 accttactac ccctcgaaga aggacggaca gttatcggat cagccgcaag agacatatct
     3901 ctccagggcc cgggtctggc cccagagcat tgctatatcg agaacctacg agggactcta
     3961 actctgtacc catgtggtaa cgcatgcacc atcgacggac ttcccgtccg ccaaccgaca
     4021 cgtctgacac agggctgcat gctgtgtctg ggccagtcca ctttcttaag gttcaaccat
     4081 ccagcagaag cgaagtggat gaaaagtatg atcccggctg gaggacgcgc tccgggaccg
     4141 ccatactccc ctgtcccagc cgaaagtgag tcgctggtca atggtaacca cactccacag
     4201 accgctactc gcggtccttc cgcttgcgcc tcccattcaa gtcttgtgtc ctcgattgaa
     4261 aaggatctcc aagaaattat ggattcgctg gtcctcgagg aaccaggcgc ggcgggcaaa
     4321 aagccggcag caacaagtcc cctctcgcca atggcgaacg gtgggcgcta cttgctgtcc
     4381 ccaccaactt cgcctggagc aatgtccgta ggttccagtt atgagaatac ctcgcccgct
     4441 ttctcacccc tatcttctcc cgcttcatca ggctcctgtg ctagccacag tcctagtggg
     4501 caagagcccg gaccttcagt cccacctctg gtacctgcga ggtccagctc ttatcacctg
     4561 gcactgcaac cgccgcagtc ccgcccatct ggtgccagaa gcgaaagccc acgacttagt
     4621 agaaagggtg ggcacgaacg tcctcccagc ccaggactcc ggggattatt gacagattcc
     4681 cctgcggcca cagtactcgc tgaggcgcgc cgcgccactg agagtccacg tctgggagga
     4741 cagttgccag tcgtggccat aagcttgtct gagtaccccg catcaggtgc tctctcccaa
     4801 cctaccagta taccaggctc accgaagttc caaccgccgg tccctgcccc tcggaacaag
     4861 atcggcacac tccaggacag gccgccttct ccctttaggg aaccaccagg atccgaacgc
     4921 gttttgacca ccagcccctc tagacagtta gttggaagaa ctttcagtga cggtcttgcc
     4981 accagaaccc ttcagccacc cgaaagtcct aggttgggcc ggaggggcct ggatagtatg
     5041 cgtgaacttc caccactaag cccgagcttg tcacggaggg cgctatcacc cctgccgacc
     5101 aggactacac cggatcctaa gttgaacagg gaagttgcag aatcacccag gccacgtcgc
     5161 tgggctgcac acggcgctag tcccgaggac ttctctctga ctcttggagc gcgtggccga
     5221 cgcaccagga gcccttcccc tacacttggc gaaagccttg ctccacacaa ggggagtttt
     5281 agtggaagac tgagccctgc ctactccttg gggagtctca ccggcgcttc accgtgccaa
     5341 tcgccatgtg ttcaacgaaa attatcatca ggagacctac gagtgccagt gacgagagaa
     5401 agaaagaatt ccattactga aatcagcgac aatgaagacg acctgctcga ataccaccga
     5461 cgacaacggc aagaaagact ccgagaacaa gagatggaga gacttgagcg ccagcggctg
     5521 gaaaccattc tcaatttatg tgctgagtat tctcgcgcag atggcggccc tgaagcgggt
     5581 gaactgccta gcataggcga agccacagcc gctcttgctc ttgcaggccg ccgacctagt
     5641 cgaggattgg ccggcgcgag tggacgctcg tcagaagagc cgggagtggc gactcagaga
     5701 ctgtgggaaa gcatggagcg gtcggacgag gagaacctga aagaggaatg tagttcaacc
     5761 gagagtaccc agcaagagca cgaggatgct ccctctacga aattgcaggg cgaagtactg
     5821 gctcttgagg aggagagagc tcaggtatta ggtcatgttg agcaacttaa ggtgagggtg
     5881 aaagagctgg aacaacaact tcaggagagt gcaagggaag cagaaatgga gagggcatta
     5941 cttcagggcg agcgcgaggc tgagagagct ctcttgcaga aggagcagaa ggctgtggac
     6001 cagctacagg agaaacttgt tgccttagag acgggaattc agaaggaaag ggataaagag
     6061 gcggaagccc tcgagacaga gacaaaactt ttcgaggatc tcgaatttca acagctcgaa
     6121 agagagagtc gagtggaaga ggaacgtgag cttgctggac aggggcttct gagaagtaag
     6181 gcagaactat tgcgttccat tgctaaaagg aaagagcgac tggctatcct ggatagccag
     6241 gcaggacaga tcagagctca ggcagttcaa gaaagcgaac gcttagcacg cgacaagaac
     6301 gcgtcactgc aactgctgca gaaggaaaaa gagaagctca cggttttgga acgtcgatat
     6361 cactctctga ccggaggcag accattcccc aaaactacgt ccacactcaa ggagatggaa
     6421 aagttattgc tacctgctgt ggacctcgaa cagtggtatc aagaacttat ggccggacta
     6481 ggcacgggtc ccgccgccgc cagtcctcac tcttcgcctc caccattacc cgccaaagcg
     6541 tcccggcagc ttcaggttta caggagcaag atggatggcg aggcaacgtc tcctttgcca
     6601 cgcactcgct cagggccgtt acccagctct tccggttcga gtagttcaag ctctcagctt
     6661 tcggtggcca cgctagggcg atcccctagt ccgaagtctg ccctgttgac ccagaatggc
     6721 acaggttcat taccacgcaa tctggcagcc accctgcagg atattgagac caagcgccag
     6781 ctggcgttgc agcagaaggg ccagcaagtg attgaggaac agagaaggag actagcagaa
     6841 ttaaaacaga aggccgccgc tgaagcccag tgccaatggg acgccctcca cggagctgca
     6901 ccttttcctg ctgggccaag cggatttcca cccctcatgc accactctat actgcatcat
     6961 ctgcccgcag gacgtgagcg cggagaagag ggcgagcacg cctatgatac tttgagtctt
     7021 gagtcaagcg atagcatgga gacatccatc tcgacgggtg ggaacagcgc ctgctcaccc
     7081 gacaatatgt cctccgccag cggcttagat atgggcaaaa ttgaggagat ggagaagatg
     7141 cttaaagagg ctcacgcaga gaagaaccgg ctgatggagt caagggagcg ggaaatggag
     7201 ctacggagac aagcccttga ggaagaacgt agacggcgcg agcaagtgga gaggaggctg
     7261 cagagtgaat ctgctaggag gcaacagctc gttgaaaaag aggttaaaat gagagaaaaa
     7321 cagttttcac aagctaggcc cctgactcgg taccttccta ttaggaaaga agactttgac
     7381 ctgaagacgc acatcgaaag cagcgggcac ggtgtcgaca cctgcctgca cgtggtgctc
     7441 agcagcaaag tgtgcagagg gtatttggtg aagatgggtg gcaagatcaa atcatggaag
     7501 aaacgctggt ttgtcttcga tcgcttgaag cgtactctct cctattacgt tgacaagcac
     7561 gagaccaagt tgaaaggggt aatctatttt caggccatcg aggaggtgta ctatgaccat
     7621 ctgcgtagtg ccgcaaagaa acggttcttc cggttcacca tggttacaga atcgccgaac
     7681 cctgccctga ccttctgcgt gaaaactcac gatcgtctgt attacatggt ggccccgtcc
     7741 gcggaggcga tgcgcatctg gatggatgtg atcgttacag gtgcggaagg atacacccag
     7801 ttcatgaatt gaaacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc
     7861 cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7921 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7981 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     8041 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     8101 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     8161 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     8221 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     8281 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     8341 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     8401 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     8461 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     8521 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     8581 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     8641 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     8701 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     8761 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     8821 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     8881 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     8941 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
     9001 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     9061 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     9121 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     9181 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     9241 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     9301 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     9361 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     9421 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     9481 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     9541 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     9601 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta
     9661 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     9721 gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact
     9781 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9841 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9901 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9961 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
    10021 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10081 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10141 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
    10201 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    10261 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    10321 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
    10381 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
    10441 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
    10501 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
    10561 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    10621 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    10681 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    10741 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    10801 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    10861 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    10921 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
    10981 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    11041 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    11101 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    11161 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    11221 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    11281 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    11341 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    11401 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    11461 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    11521 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    11581 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    11641 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    11701 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    11761 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    11821 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    11881 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    11941 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    12001 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    12061 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt cca
//
