LOCUS       sequence-205648-       11453 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation T233F with approximately
            wildtype activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTT233F-myc
FEATURES             Location/Qualifiers
     source          1..11453
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(693..711)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        779..883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(884..952,953..1744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1102..1121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1915..2503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2404..2423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2934..3079
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3122..4159
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4157..4176)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4201..4249
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4903..5282
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5283..5486
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5437..5457
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5483..5507
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5535..5544
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6210..6239
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7528..7662
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7552..7571)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7606..7625
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7805..7827)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8387..10176
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga caccatctct
       61 atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg
      121 tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc cactgccgcc
      181 ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag gtcctgtacc
      241 tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt tattagacga
      301 cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc ccaccctcat
      361 ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc tccgaaccat
      421 cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca tcctcaagat
      481 ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg ctggacggta
      541 gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac agagatgcta
      601 ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc gatgataagc
      661 tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      721 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      781 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      841 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      901 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      961 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1021 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1081 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     1141 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1201 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1261 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1321 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1381 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     1441 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1501 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1561 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1621 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1681 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1741 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1801 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1861 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1921 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1981 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2041 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     2101 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2161 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2221 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2281 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2341 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2401 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2461 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2521 ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac
     2581 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
     2641 cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc
     2701 gtcggccccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc
     2761 tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt
     2821 ggagtggtga atccgttagc gaggtgccgc cctgcttcat ccccgtggcc cgttgctcgc
     2881 gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct atgatgacac
     2941 aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc
     3001 gcggtccgag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac
     3061 cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga
     3121 tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     3181 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     3241 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     3301 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     3361 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     3421 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     3481 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     3541 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     3601 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     3661 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     3721 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     3781 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     3841 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     3901 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     3961 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     4021 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     4081 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cggatcggga
     4141 gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     4201 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
     4261 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     4321 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     4381 gcagccaacg tcggggcggc aggccctgcc atagccactg gccccgtggg ttagggacgg
     4441 ggtcccccat ggggaatggt ttatggttcg tgggggttat tattttgggc gttgcgtggg
     4501 gtcaggtcca cgactggact gagcagacag acccatggtt tttggatggc ctgggcatgg
     4561 accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac acccccgacc
     4621 cccaaaaacc accgcgcgga tttctggcgt gccaagctag tcgaccaatt ctcatgtttg
     4681 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
     4741 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
     4801 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
     4861 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
     4921 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     4981 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     5041 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     5101 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     5161 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     5221 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     5281 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
     5341 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     5401 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
     5461 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct agaagctggg
     5521 taccagctgc tagcgccacc atggagacca cccccctgaa cagtcaaaag caactctctg
     5581 cctgtgagga tggagaggac tgtcaggaga atggcgttct tcaaaaggtt gtacccaccc
     5641 ctggagataa ggtggaaagc ggccagattt ccaacggcta cagcgccgtg ccatcacctg
     5701 gagccgggga tgatacacgg cacagcattc ccgcaaccac cacgacactg gttgcagagc
     5761 ttcaccaggg cgagagggag acgtggggca aaaaggtaga ttttctgctt tccgtgatcg
     5821 gttacgcggt cgatctgggg aacgtgtgga ggttccctta catttgttac caaaacggag
     5881 ggggtgcctt cttgctgccc tacacaatta tggcgatttt tgggggtatc ccgctcttct
     5941 acatggaact ggccttgggt cagtaccacc ggaacggctg cattagcatt tggcgcaaga
     6001 tatgccccat tttcaaggga atcggatacg caatctgtat aatcgcgttc tatattgcat
     6061 catattacaa caccatcatg gcctgggccc tttattactt gatcagcagt ttcaccgacc
     6121 aattgccgtg gacgagctgt aagaactcat ggaataccgg gaactgtacc aactacttct
     6181 ctgaggacag ctcaggaggt agcgggggtg aacagaagct gattagcgag gaggacttgg
     6241 gcagtccagg cggaagcggc aacatcactt ggacactcca tagtacgagc cccgcagagg
     6301 agttttactt caggcacgta ctgcagattc ataggagcaa gggtctgcaa gacctgggcg
     6361 gtattagctg gcagctcgca ttgtgcataa tgttgatctt tacggtgatc tatttctcta
     6421 tctggaaggg agtaaagaca tccggcaagg tggtgtgggt gacggcgacc ttcccctaca
     6481 ttatactcag cgtgttgttg gtgagggggg caaccctgcc tggggcctgg cgaggcgtgc
     6541 tgttctatct caagccaaac tggcagaagc ttcttgaaac cggagtgtgg atcgacgcgg
     6601 ccgcgcaaat cttcttttcc ctcggtcccg gcttcggggt ccttctggca ttcgcaagct
     6661 acaacaagtt caacaataac tgctaccagg acgccctcgt gacaagtgta gtgaactgca
     6721 tgacctcttt cgtgagcggg tttgtgatct tcacagtact cggatatatg gctgagatga
     6781 ggaacgaaga tgttagtgag gttgccaaag acgctggccc gagcctgttg ttcatcacat
     6841 acgccgaggc tatcgcgaac atgcccgcat ctactttctt cgccatcatc tttttcctga
     6901 tgctgataac ccttgggctg gatagcacct tcgccggact ggagggtgtc atcactgccg
     6961 ttctcgacga gttcccccat gtgtgggcca agcgacggga aaggttcgtg ctggccgtcg
     7021 taatcacatg cttcttcggc tcactggtga cccttacctt tggcggtgcg tacgtggtca
     7081 agctgctcga agagtacgcc actggaccgg cggttctgac cgtggcattg atcgaggccg
     7141 tggccgtaag ctggttttac ggcatcacgc agttctgtag ggacgtgaaa gagatgctgg
     7201 gcttttctcc cggttggttt tggaggattt gttgggtagc aattagcccc ctgtttctcc
     7261 ttttcattat ttgctctttt ctgatgtctc caccgcagct ccgactgttt cagtacaact
     7321 acccctactg gtcaattatc ctgggctatt gtatcggcac ctcaagtttc atctgcatcc
     7381 ccacatacat cgcatacaga ctgataatta cgcccggtac ttttaaggag cgaatcatta
     7441 aaagcataac ccccgaaacc cccaccgaga tcccttgtgg cgatataaga ctcaatgcgg
     7501 tctaatagct cgaggccggc aaggccggat ccagacatga taagatacat tgatgagttt
     7561 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     7621 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     7681 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     7741 tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg
     7801 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     7861 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     7921 tgaggtcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     7981 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     8041 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     8101 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     8161 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     8221 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     8281 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     8341 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     8401 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     8461 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     8521 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     8581 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     8641 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     8701 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     8761 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     8821 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     8881 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     8941 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     9001 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     9061 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     9121 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     9181 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     9241 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     9301 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     9361 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     9421 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     9481 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     9541 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     9601 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     9661 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     9721 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     9781 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     9841 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     9901 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     9961 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
    10021 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca
    10081 gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca
    10141 agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga
    10201 atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct
    10261 aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta
    10321 tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac
    10381 accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc
    10441 ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct
    10501 catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt
    10561 ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca
    10621 ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca
    10681 aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc
    10741 ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg
    10801 gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg
    10861 tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt
    10921 ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt
    10981 tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat
    11041 caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct
    11101 ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa
    11161 aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg
    11221 gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc
    11281 cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac
    11341 cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc
    11401 cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct cct
//
