LOCUS       sequence-205649-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FITM2, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) FITM2
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(821..845)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acaagtttgt acaaaaaagc aggcttcacc atggaacact tagagcggtg cgagtggttg
       61 ttaagaggta cactggtccg agcggctgtg aggcgctatt tgccttgggc ccttgtagcc
      121 tccatgcttg ccggatctct attgaaagaa cttagccccc ttccggaatc atatttatct
      181 aataagcgca atgtattgaa cgtttatttc gttaaagttg cctgggcgtg gacgttttgc
      241 ttgctgctgc cttttatagc actaacgaat taccacctaa caggcaaggc aggccttgtc
      301 ctcaggcggc tgtctaccct cctcgtcggc accgccattt ggtacatatg tacaagtatc
      361 ttttccaaca ttgagcatta tactggcagc tgctatcaaa gtccagcact tgaaggtgtg
      421 agaaaggaac atcaatcgaa gcagcagtgc catcaggagg gcggtttttg gcacggattt
      481 gacatctccg gacactcatt tctcctcacc ttctgcgctc tgatgattgt ggaggagatg
      541 agcgtgctgc atgaggtcaa aaccgatcgt tcccactgtc tgcacacagc tatcacaact
      601 ctcgtggtgg cactcggaat cctcactttc atctgggtgc tgatgttcct gtgtactgcc
      661 gtgtacttcc ataacctgag ccagaaagtg tttgggaccc tgttcgggct gctgtcttgg
      721 tacggcacct acgggttctg gtacccaaag gctttctccc ccgggctgcc ccctcagtca
      781 tgtagcctga acctgaagca ggacagttac aagaaatgaa acccagcttt cttgtacaaa
      841 gtggt
//
