LOCUS       sequence-205655-       11521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation R596Q conferring gain of
            function phenotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTR596Q-myc
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(715..733)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        801..905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(906..974,975..1766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1124..1143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1937..2525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2426..2445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2956..3101
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3144..4181
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4179..4198)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4223..4271
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4925..5304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5305..5508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5459..5479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5505..5529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5557..5566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6232..6261
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7550..7684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7574..7593)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7628..7647
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7827..7849)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8409..10198
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt
       61 ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg
      121 gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc
      181 ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta
      241 ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc
      301 aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct
      361 ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa
      421 aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac
      481 tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg
      541 tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca
      601 aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag
      661 gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg
      721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1141 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1441 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct
     2581 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     2641 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
     2701 ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat
     2761 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     2821 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc
     2881 atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat
     2941 gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac
     3001 acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt
     3061 gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg
     3121 cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     3181 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     3241 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     3301 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     3361 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     3421 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     3481 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     3541 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     3601 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     3661 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     3721 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     3781 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     3841 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     3901 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     3961 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     4021 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     4081 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     4141 ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata
     4201 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     4261 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     4321 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     4381 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac
     4441 tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt
     4501 attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg
     4561 tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg
     4621 tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct
     4681 agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     4741 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     4801 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     4861 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     4921 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4981 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     5041 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     5101 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     5161 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     5221 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     5281 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
     5341 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     5401 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg
     5461 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     5521 cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatggagac cacccccctg
     5581 aacagtcaaa agcaactctc tgcctgtgag gatggagagg actgtcagga gaatggcgtt
     5641 cttcaaaagg ttgtacccac ccctggagat aaggtggaaa gcggccagat ttccaacggc
     5701 tacagcgccg tgccatcacc tggagccggg gatgatacac ggcacagcat tcccgcaacc
     5761 accacgacac tggttgcaga gcttcaccag ggcgagaggg agacgtgggg caaaaaggta
     5821 gattttctgc tttccgtgat cggttacgcg gtcgatctgg ggaacgtgtg gaggttccct
     5881 tacatttgtt accaaaacgg agggggtgcc ttcttgctgc cctacacaat tatggcgatt
     5941 tttgggggta tcccgctctt ctacatggaa ctggccttgg gtcagtacca ccggaacggc
     6001 tgcattagca tttggcgcaa gatatgcccc attttcaagg gaatcggata cgcaatctgt
     6061 ataatcgcgt tctatattgc atcatattac aacaccatca tggcctgggc cctttattac
     6121 ttgatcagca gtttcaccga ccaattgccg tggacgagct gtaagaactc atggaatacc
     6181 gggaactgta ccaactactt ctctgaggac agctcaggag gtagcggggg tgaacagaag
     6241 ctgattagcg aggaggactt gggcagtcca ggcggaagcg gcaacatcac ttggacactc
     6301 catagtacga gccccgcaga ggagttttac accaggcacg tactgcagat tcataggagc
     6361 aagggtctgc aagacctggg cggtattagc tggcagctcg cattgtgcat aatgttgatc
     6421 tttacggtga tctatttctc tatctggaag ggagtaaaga catccggcaa ggtggtgtgg
     6481 gtgacggcga ccttccccta cattatactc agcgtgttgt tggtgagggg ggcaaccctg
     6541 cctggggcct ggcgaggcgt gctgttctat ctcaagccaa actggcagaa gcttcttgaa
     6601 accggagtgt ggatcgacgc ggccgcgcaa atcttctttt ccctcggtcc cggcttcggg
     6661 gtccttctgg cattcgcaag ctacaacaag ttcaacaata actgctacca ggacgccctc
     6721 gtgacaagtg tagtgaactg catgacctct ttcgtgagcg ggtttgtgat cttcacagta
     6781 ctcggatata tggctgagat gaggaacgaa gatgttagtg aggttgccaa agacgctggc
     6841 ccgagcctgt tgttcatcac atacgccgag gctatcgcga acatgcccgc atctactttc
     6901 ttcgccatca tctttttcct gatgctgata acccttgggc tggatagcac cttcgccgga
     6961 ctggagggtg tcatcactgc cgttctcgac gagttccccc atgtgtgggc caagcgacgg
     7021 gaaaggttcg tgctggccgt cgtaatcaca tgcttcttcg gctcactggt gacccttacc
     7081 tttggcggtg cgtacgtggt caagctgctc gaagagtacg ccactggacc ggcggttctg
     7141 accgtggcat tgatcgaggc cgtggccgta agctggtttt acggcatcac gcagttctgt
     7201 agggacgtga aagagatgct gggcttttct cccggttggt tttggaggat ttgttgggta
     7261 gcaattagcc ccctgtttct ccttttcatt atttgctctt ttctgatgtc tccaccgcag
     7321 ctccgactgt ttcagtacaa ctacccctac tggtcaatta tcctgggcta ttgtatcggc
     7381 acctcaagtt tcatctgcat ccccacatac atcgcatacc aactgataat tacgcccggt
     7441 acttttaagg agcgaatcat taaaagcata acccccgaaa cccccaccga gatcccttgt
     7501 ggcgatataa gactcaatgc ggtctaatag ctcgaggccg gcaaggccgg atccagacat
     7561 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     7621 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     7681 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     7741 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc cggctgcctc
     7801 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     7861 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     7921 ggcgggtgtc ggggcgcagc catgaggtcg actctagagg atcgatcccc gccccggacg
     7981 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     8041 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     8101 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     8161 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     8221 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     8281 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     8341 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     8401 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     8461 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     8521 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     8581 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     8641 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     8701 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     8761 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     8821 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     8881 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     8941 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     9001 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     9061 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     9121 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     9181 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     9241 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     9301 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     9361 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     9421 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     9481 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     9541 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     9601 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     9661 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     9721 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     9781 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     9841 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     9901 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     9961 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
    10021 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
    10081 tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct
    10141 ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct
    10201 ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat
    10261 tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct
    10321 gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga
    10381 caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct
    10441 ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat
    10501 cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca
    10561 tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca
    10621 tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca
    10681 aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg
    10741 aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt
    10801 ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct
    10861 cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa
    10921 gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct
    10981 gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct
    11041 ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct
    11101 gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg
    11161 ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt
    11221 ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc
    11281 ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc
    11341 ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca
    11401 ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct
    11461 gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc ccctcccgct
    11521 c
//
