LOCUS       sequence-205656-        8600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing RALB, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 8600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) RALB
FEATURES             Location/Qualifiers
     source          1..8600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      24..612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     513..532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     766..783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    900..921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(936..941,942..959,960..966)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    974..990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     979..1001
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     998..1014
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     998..1014
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1033..1053
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1035..1053
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1081..1307
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1308..1488
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1535..1660
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2153..2386
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2571..2615
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2882..2998
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3021..3324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3325..3528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3479..3499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3525..3549
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3633..3657
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4285..4309)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4340..4928
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4393..4413)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4811..4822)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4942..5441
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4977..5000)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5374..5393
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5462..6061
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5462..5481)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5958..5978
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6199..6432
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6504..6625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6541..6560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6595..6614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6665..6800
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6820..6839)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6821..6839)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6849..6866)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6849..6865)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6858..6880)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7007..7462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7094..7113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7304..7325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7488..7592
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7593..7661,7662..8453)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7811..7830)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
       61 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      781 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      841 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      901 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      961 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     1021 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     1081 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     1141 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     1201 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     1261 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     1321 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     1381 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1441 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     1501 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     1561 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1621 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1681 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1741 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1801 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1861 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1921 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1981 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2041 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     2101 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     2161 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     2221 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     2281 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     2341 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     2401 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     2461 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     2521 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2581 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2641 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2701 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2761 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2821 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     2881 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2941 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     3001 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3061 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3301 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3361 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3421 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3481 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     3541 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     3601 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttca
     3661 ccatggctgc caacaagagt aagggccaga gctccttggc cctccacaag gtgatcatgg
     3721 ttggcagcgg aggcgttggc aagtcagccc tgacgcttca gttcatgtat gacgagtttg
     3781 tagaagacta tgaacctacc aaagctgaca gttatagaaa gaaagtggtt cttgatgggg
     3841 aagaagttca gatagatatt ctggacaccg ctgggcaaga ggactacgca gccattcgag
     3901 ataactactt tcggagtggg gaagggtttc ttcttgtgtt ctcaatcaca gaacatgaat
     3961 cctttacagc aactgccgaa ttcagggaac agattctccg tgtgaaggct gaagaagata
     4021 aaattccact gctcgtcgtg ggaaacaagt ctgacctaga ggagcggagg caggtgcctg
     4081 tggaggaggc caggagtaaa gccgaagagt ggggcgtgca gtacgtggag acgtcagcga
     4141 agacccgggc caacgtggac aaggtgttct ttgacctaat gagagaaatc agaacaaaga
     4201 agatgtcaga aaacaaagac aagaatggca agaaaagcag caagaacaag aaaagtttta
     4261 aagaaagatg ttgcttacta tgaaacccag ctttcttgta caaagtggtt gatatccagc
     4321 acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4381 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4441 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4501 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4561 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4621 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4681 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     4741 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4801 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4861 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4921 ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     4981 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     5041 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     5101 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     5161 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     5221 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     5281 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     5341 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     5401 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     5461 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     5521 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     5581 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5641 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5701 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     5761 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5821 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5881 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     5941 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6001 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6061 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg
     6121 agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6181 taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt
     6241 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6301 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6361 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6421 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6481 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     6541 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6601 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     6661 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6721 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6781 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     6841 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6901 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6961 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     7021 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7081 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7141 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7201 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7261 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7321 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7381 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7441 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7501 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7561 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7621 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7681 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7741 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7801 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7861 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7921 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7981 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8041 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8101 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8161 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8221 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8281 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8341 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8401 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8461 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8521 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8581 tccactgagc gtcagacccc
//
