LOCUS       sequence-205657-       11510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation V343T conffering gain of
            function phenotype, decreased surface expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTV343T-myc
FEATURES             Location/Qualifiers
     source          1..11510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(721..739)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        807..911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(912..980,981..1772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1130..1149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1943..2531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2432..2451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2962..3107
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3150..4187
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4185..4204)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4229..4277
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4931..5310
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5311..5514
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5465..5485
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5511..5535
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5563..5572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6238..6267
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7556..7690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7580..7599)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7634..7653
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7833..7855)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8415..10204
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac
       61 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg
      121 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac
      181 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct
      241 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa
      301 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg
      361 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg
      421 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg
      481 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat
      541 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc
      601 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc
      661 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
      721 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      781 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      841 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      901 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      961 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1021 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1081 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1141 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1201 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1261 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1321 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1381 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1441 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1501 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1561 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1621 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1681 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1741 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1801 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1861 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1921 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1981 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2041 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2101 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2161 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2221 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2281 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2341 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2401 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2461 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2521 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2581 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
     2641 tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg
     2701 cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct
     2761 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     2821 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc
     2881 tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat
     2941 ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat
     3001 tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg
     3061 acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc
     3121 gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
     3181 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     3241 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     3301 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     3361 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     3421 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     3481 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     3541 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     3601 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     3661 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     3721 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     3781 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     3841 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     3901 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     3961 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     4021 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     4081 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     4141 gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag
     4201 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
     4261 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     4321 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     4381 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     4441 agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg
     4501 ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac
     4561 ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg
     4621 cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc
     4681 caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg
     4741 ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat
     4801 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
     4861 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
     4921 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     4981 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     5041 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     5101 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     5161 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
     5221 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
     5281 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
     5341 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     5401 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
     5461 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     5521 gtgaaccgtc agatctctag aagctgggta ccagctgcta gcgccaccat ggagaccacc
     5581 cccctgaaca gtcaaaagca actctctgcc tgtgaggatg gagaggactg tcaggagaat
     5641 ggcgttcttc aaaaggttgt acccacccct ggagataagg tggaaagcgg ccagatttcc
     5701 aacggctaca gcgccgtgcc atcacctgga gccggggatg atacacggca cagcattccc
     5761 gcaaccacca cgacactggt tgcagagctt caccagggcg agagggagac gtggggcaaa
     5821 aaggtagatt ttctgctttc cgtgatcggt tacgcggtcg atctggggaa cgtgtggagg
     5881 ttcccttaca tttgttacca aaacggaggg ggtgccttct tgctgcccta cacaattatg
     5941 gcgatttttg ggggtatccc gctcttctac atggaactgg ccttgggtca gtaccaccgg
     6001 aacggctgca ttagcatttg gcgcaagata tgccccattt tcaagggaat cggatacgca
     6061 atctgtataa tcgcgttcta tattgcatca tattacaaca ccatcatggc ctgggccctt
     6121 tattacttga tcagcagttt caccgaccaa ttgccgtgga cgagctgtaa gaactcatgg
     6181 aataccggga actgtaccaa ctacttctct gaggacagct caggaggtag cgggggtgaa
     6241 cagaagctga ttagcgagga ggacttgggc agtccaggcg gaagcggcaa catcacttgg
     6301 acactccata gtacgagccc cgcagaggag ttttacacca ggcacgtact gcagattcat
     6361 aggagcaagg gtctgcaaga cctgggcggt attagctggc agctcgcatt gtgcataatg
     6421 ttgatcttta cggtgatcta tttctctatc tggaagggag taaagacatc cggcaaggtg
     6481 gtgtgggtga cggcgacctt cccctacatt atactcagcg tgttgttggt gaggggggca
     6541 accctgcctg gggcctggcg aggcgtgctg ttctatctca agccaaactg gcagaagctt
     6601 cttgaaaccg gagtgtggat cgacgcggcc gcgcaaatct tcttttccct cggtcccggc
     6661 ttcgggaccc ttctggcatt cgcaagctac aacaagttca acaataactg ctaccaggac
     6721 gccctcgtga caagtgtagt gaactgcatg acctctttcg tgagcgggtt tgtgatcttc
     6781 acagtactcg gatatatggc tgagatgagg aacgaagatg ttagtgaggt tgccaaagac
     6841 gctggcccga gcctgttgtt catcacatac gccgaggcta tcgcgaacat gcccgcatct
     6901 actttcttcg ccatcatctt tttcctgatg ctgataaccc ttgggctgga tagcaccttc
     6961 gccggactgg agggtgtcat cactgccgtt ctcgacgagt tcccccatgt gtgggccaag
     7021 cgacgggaaa ggttcgtgct ggccgtcgta atcacatgct tcttcggctc actggtgacc
     7081 cttacctttg gcggtgcgta cgtggtcaag ctgctcgaag agtacgccac tggaccggcg
     7141 gttctgaccg tggcattgat cgaggccgtg gccgtaagct ggttttacgg catcacgcag
     7201 ttctgtaggg acgtgaaaga gatgctgggc ttttctcccg gttggttttg gaggatttgt
     7261 tgggtagcaa ttagccccct gtttctcctt ttcattattt gctcttttct gatgtctcca
     7321 ccgcagctcc gactgtttca gtacaactac ccctactggt caattatcct gggctattgt
     7381 atcggcacct caagtttcat ctgcatcccc acatacatcg catacagact gataattacg
     7441 cccggtactt ttaaggagcg aatcattaaa agcataaccc ccgaaacccc caccgagatc
     7501 ccttgtggcg atataagact caatgcggtc taatagctcg aggccggcaa ggccggatcc
     7561 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     7621 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     7681 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     7741 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatccggc
     7801 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     7861 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7921 ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc tagaggatcg atccccgccc
     7981 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     8041 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     8101 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     8161 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     8221 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     8281 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     8341 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     8401 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     8461 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     8521 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     8581 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     8641 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     8701 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     8761 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     8821 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     8881 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     8941 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     9001 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     9061 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     9121 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     9181 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     9241 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     9301 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     9361 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     9421 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     9481 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     9541 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     9601 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     9661 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     9721 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     9781 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     9841 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     9901 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     9961 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
    10021 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
    10081 atatgctatc ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc
    10141 tatcctttgc atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt
    10201 cctgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg
    10261 tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc
    10321 ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa
    10381 tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct actggggatt
    10441 tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag
    10501 ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct
    10561 ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct
    10621 actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc
    10681 ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag
    10741 accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg
    10801 gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca
    10861 gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg
    10921 acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa
    10981 ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac
    11041 caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct
    11101 tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca
    11161 tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat
    11221 ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg
    11281 ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt
    11341 ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg
    11401 cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct
    11461 gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
//
