LOCUS       sequence-20566-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG424GPD-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(16..140)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(148..164)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(148..164)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(170..433)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(253..276)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 cctaatagcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
       61 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      121 gcttttttgt acaaacttgt gatgggggat ccactagttc tagaatccgt cgaaactaag
      181 ttctggtgtt ttaaaactaa aaaaaagact aactataaaa gtagaattta agaagtttaa
      241 gaaatagatt tacagaatta caatcaatac ctaccgtctt tatatactta ttagtcaagt
      301 aggggaataa tttcagggaa ctggtttcaa cctttttttt cagctttttc caaatcagag
      361 agagcagaag gtaatagaag gtgtaagaaa atgagataga tacatgcgtg ggtcaattgc
      421 cttgtgtcat catttactcc aggcaggttg catcactcca ttgaggttgt gcccgttttt
      481 tgcctgtttg tgcccctgtt ctctgtagtt gcgctaagag aatggaccta tgaactgatg
      541 gttggtgaag aaaacaatat tttggtgctg ggattctttt tttttctgga tgccagctta
      601 aaaagcgggc tccattatat
//
