LOCUS       sequence-205661-       11557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation I379Q conferring gain of
            function phenotype, decreased surface expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTI379Q-myc
FEATURES             Location/Qualifiers
     source          1..11557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(783..801)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        869..973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(974..1042,1043..1834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1192..1211)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2005..2593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2494..2513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3024..3169
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3212..4249
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4247..4266)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4291..4339
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4993..5372
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5373..5576
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5527..5547
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5573..5597
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5625..5634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6300..6329
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7618..7752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7642..7661)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7696..7715
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7895..7917)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8477..10266
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc
       61 tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg
      121 acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg
      181 ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc
      241 accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt
      301 ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa
      361 aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc
      421 tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca
      481 ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc
      541 cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa
      601 attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt
      661 cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg
      721 cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
      781 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      841 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      901 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      961 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1021 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1081 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1141 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1201 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     1261 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1321 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1381 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1441 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1501 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1561 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1621 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1681 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1741 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1801 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1861 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1921 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1981 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2041 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2101 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     2161 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2221 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2281 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2341 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2401 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2461 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2521 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2581 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2641 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
     2701 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
     2761 cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg
     2821 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
     2881 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
     2941 cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat
     3001 atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga
     3061 attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg
     3121 tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca
     3181 gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
     3241 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     3301 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     3361 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     3421 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     3481 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     3541 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     3601 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     3661 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     3721 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     3781 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     3841 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     3901 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     3961 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     4021 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     4081 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     4141 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     4201 ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg
     4261 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     4321 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     4381 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     4441 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     4501 atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg
     4561 tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag
     4621 acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg
     4681 ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt
     4741 gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     4801 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     4861 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     4921 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     4981 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     5041 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     5101 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     5161 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     5221 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
     5281 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
     5341 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
     5401 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5461 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
     5521 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     5581 tagtgaaccg tcagatctct agaagctggg taccagctgc tagcgccacc atggagacca
     5641 cccccctgaa cagtcaaaag caactctctg cctgtgagga tggagaggac tgtcaggaga
     5701 atggcgttct tcaaaaggtt gtacccaccc ctggagataa ggtggaaagc ggccagattt
     5761 ccaacggcta cagcgccgtg ccatcacctg gagccgggga tgatacacgg cacagcattc
     5821 ccgcaaccac cacgacactg gttgcagagc ttcaccaggg cgagagggag acgtggggca
     5881 aaaaggtaga ttttctgctt tccgtgatcg gttacgcggt cgatctgggg aacgtgtgga
     5941 ggttccctta catttgttac caaaacggag ggggtgcctt cttgctgccc tacacaatta
     6001 tggcgatttt tgggggtatc ccgctcttct acatggaact ggccttgggt cagtaccacc
     6061 ggaacggctg cattagcatt tggcgcaaga tatgccccat tttcaaggga atcggatacg
     6121 caatctgtat aatcgcgttc tatattgcat catattacaa caccatcatg gcctgggccc
     6181 tttattactt gatcagcagt ttcaccgacc aattgccgtg gacgagctgt aagaactcat
     6241 ggaataccgg gaactgtacc aactacttct ctgaggacag ctcaggaggt agcgggggtg
     6301 aacagaagct gattagcgag gaggacttgg gcagtccagg cggaagcggc aacatcactt
     6361 ggacactcca tagtacgagc cccgcagagg agttttacac caggcacgta ctgcagattc
     6421 ataggagcaa gggtctgcaa gacctgggcg gtattagctg gcagctcgca ttgtgcataa
     6481 tgttgatctt tacggtgatc tatttctcta tctggaaggg agtaaagaca tccggcaagg
     6541 tggtgtgggt gacggcgacc ttcccctaca ttatactcag cgtgttgttg gtgagggggg
     6601 caaccctgcc tggggcctgg cgaggcgtgc tgttctatct caagccaaac tggcagaagc
     6661 ttcttgaaac cggagtgtgg atcgacgcgg ccgcgcaaat cttcttttcc ctcggtcccg
     6721 gcttcggggt ccttctggca ttcgcaagct acaacaagtt caacaataac tgctaccagg
     6781 acgccctcgt gacaagtgta gtgaactgca tgacctcttt cgtgagcggg tttgtgcaat
     6841 tcacagtact cggatatatg gctgagatga ggaacgaaga tgttagtgag gttgccaaag
     6901 acgctggccc gagcctgttg ttcatcacat acgccgaggc tatcgcgaac atgcccgcat
     6961 ctactttctt cgccatcatc tttttcctga tgctgataac ccttgggctg gatagcacct
     7021 tcgccggact ggagggtgtc atcactgccg ttctcgacga gttcccccat gtgtgggcca
     7081 agcgacggga aaggttcgtg ctggccgtcg taatcacatg cttcttcggc tcactggtga
     7141 cccttacctt tggcggtgcg tacgtggtca agctgctcga agagtacgcc actggaccgg
     7201 cggttctgac cgtggcattg atcgaggccg tggccgtaag ctggttttac ggcatcacgc
     7261 agttctgtag ggacgtgaaa gagatgctgg gcttttctcc cggttggttt tggaggattt
     7321 gttgggtagc aattagcccc ctgtttctcc ttttcattat ttgctctttt ctgatgtctc
     7381 caccgcagct ccgactgttt cagtacaact acccctactg gtcaattatc ctgggctatt
     7441 gtatcggcac ctcaagtttc atctgcatcc ccacatacat cgcatacaga ctgataatta
     7501 cgcccggtac ttttaaggag cgaatcatta aaagcataac ccccgaaacc cccaccgaga
     7561 tcccttgtgg cgatataaga ctcaatgcgg tctaatagct cgaggccggc aaggccggat
     7621 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     7681 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     7741 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     7801 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     7861 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7921 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7981 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc
     8041 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     8101 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     8161 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     8221 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     8281 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     8341 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     8401 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     8461 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     8521 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     8581 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     8641 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     8701 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     8761 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     8821 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     8881 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     8941 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     9001 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     9061 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     9121 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     9181 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     9241 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     9301 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     9361 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     9421 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     9481 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     9541 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     9601 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     9661 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     9721 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     9781 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     9841 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     9901 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     9961 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
    10021 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
    10081 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
    10141 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
    10201 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
    10261 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
    10321 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
    10381 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
    10441 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
    10501 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
    10561 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
    10621 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
    10681 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
    10741 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
    10801 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
    10861 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
    10921 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
    10981 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
    11041 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
    11101 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
    11161 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
    11221 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
    11281 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
    11341 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
    11401 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
    11461 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
    11521 ctgctcctgc ccctcctgct cctgcccctc ctcctgc
//
