LOCUS       sequence-205665-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expressing VOPP1, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) VOPP1
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(501..525)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(609..629)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggtggcggcg ctgctgctcg ggctgctctt ggagtgcaca gaagccaaaa agcattgctg
       61 gtatttcgaa ggactctatc caacctatta tatatgccgc tcctacgagg actgctgtgg
      121 ctccaggtgc tgtgtgcggg ccctctccat acagaggctg tggtacttct ggttccttct
      181 gatgatgggc gtgcttttct gctgcggagc cggcttcttc atccggaggc gcatgtaccc
      241 cccgccgctg atcgaggagc cagccttcaa tgtgtcctac accaggcagc ccccaaatcc
      301 cggcccagga gcccagcagc cggggccgcc ctattacacc gacccaggag gaccggggat
      361 gaaccctgtc gggaattcca tggcaatggc tttccaggtc ccacccaact caccccaggg
      421 gagtgtggcc tgcccgcccc ctccagccta ctgcaacacg cctccgcccc cgtacgaaca
      481 ggtagtgaag gccaagtaga acccagcttt cttgtacaaa gtggttgata tccagcacag
      541 tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
      601 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
      661 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
      721 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
      781 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
      841 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
      901 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
      961 tttccatgg
//
