LOCUS       sequence-205668-       10622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing EPB41L1, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) EPB41L1
FEATURES             Location/Qualifiers
     source          1..10622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      16..604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     505..524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     758..775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    892..913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(928..933,934..951,952..958)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    966..982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     971..993
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     990..1006
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     990..1006
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1025..1045
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1027..1045
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1073..1299
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1300..1480
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1527..1652
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2145..2378
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2563..2607
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2874..2990
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3013..3316
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3317..3520
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3471..3491
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3517..3541
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3625..3649
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6299..6323)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6354..6942
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6407..6427)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6825..6836)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6956..7455
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6991..7014)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7388..7407
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7476..8075
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7476..7495)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7972..7992
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8213..8446
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8518..8639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8555..8574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8609..8628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8679..8814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8834..8853)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8835..8853)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8863..8880)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8863..8879)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8872..8894)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9021..9476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9108..9127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9318..9339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9502..9606
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9607..9675,9676..10467)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9825..9844)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
       61 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      781 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      841 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      901 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      961 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1021 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     1081 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     1141 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     1201 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     1261 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     1321 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     1381 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     1441 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     1501 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     1561 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     1621 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1681 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1741 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1801 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1861 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1921 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1981 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     2041 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     2101 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     2161 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     2221 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     2281 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     2341 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     2401 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     2461 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     2521 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     2581 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     2641 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2701 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2761 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2821 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     2881 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2941 gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc
     3001 ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     3061 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     3121 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     3181 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     3241 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     3301 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     3361 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     3421 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     3481 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     3541 cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac tctagtccag
     3601 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc caccatgaca
     3661 acagagacag gccccgactc tgaggtgaag aaagctcagg aggaggcccc gcagcagccc
     3721 gaggctgctg ccgctgtgac cacccctgtg acccctgcag gccacggcca cccagaggcc
     3781 aactccaatg agaagcatcc atcccagcag gacacgcggc ctgctgaaca gagcctagac
     3841 atggaggaga aggactacag tgaggccgat ggcctttcgg agaggaccac gcccagcaag
     3901 gcccagaaat cgccccagaa gattgccaag aaatacaaga gtgccatctg ccgggtcact
     3961 ctgcttgatg cctcggagta tgagtgtgag gtggagaaac atggccgggg ccaggtgctg
     4021 tttgacctgg tctgtgaaca cctcaacctc ctagagaagg actacttcgg cctgaccttc
     4081 tgtgatgctg acagccagaa gaactggctg gacccctcca aggagatcaa gaagcagatc
     4141 cggagtagcc cctggaattt tgccttcaca gtcaagttct acccgcctga tcctgcccag
     4201 ctgacagaag acatcacaag atactacctg tgcctgcagc tgcgggcaga catcatcacg
     4261 ggccggctgc catgctcctt tgtcacgcat gccctactgg gctcctacgc tgtgcaggct
     4321 gagctgggtg actatgatgc tgaggagcat gtgggcaact atgtcagcga gctccgcttc
     4381 gcccctaacc agacccggga gctggaggag aggatcatgg agctgcataa gacatatagg
     4441 gggatgaccc cgggagaagc agaaatccac ttcttagaga atgccaagaa gctttccatg
     4501 tacggagtag acctgcacca tgccaaggac tctgagggca tcgacatcat gttaggcgtt
     4561 tgtgccaatg gcctgctcat ctaccgggac cggctgagaa tcaaccgctt tgcctggccc
     4621 aagatcctca agatctccta caagaggagt aacttctata tcaagatccg gcctggggag
     4681 tatgagcaat ttgagagcac aattggcttt aagctcccaa accaccggtc agccaagaga
     4741 ctgtggaagg tctgcatcga gcatcataca ttcttccggc tggtgtcccc tgagccccca
     4801 cccaagggct tcctggtgat gggctccaag ttccggtaca gtgggaggac ccaggcacag
     4861 actcgccagg ccagcgccct cattgaccgg cctgcaccct tctttgagcg ttcttccagc
     4921 aaacggtaca ccatgtcccg cagccttgat ggagcagagt tctcccgccc agcctcggtc
     4981 agcgagaacc atgatgcagg gcctgacggt gacaagcggg atgaggatgg cgagtctggg
     5041 gggcaacggt cagaggctga ggagggagag gtcaggactc caaccaagat caaggagcta
     5101 aagccggagc aggaaaccac gccgagacac aagcaggagt tcttagacaa gccagaagat
     5161 gtcttgctga agcaccaggc cagcatcaat gagctcaaaa ggaccctgaa ggagcccaac
     5221 agcaaactca tccaccggga tcgagactgg gaacgggagc gcaggctgcc ctcctccccc
     5281 gcctccccct cccccaaggg cacccctgag aaagccaatg agagagcagg gctgagggag
     5341 ggctccgagg agaaagtcaa accaccacgt ccccgggccc cagagagtga cacaggcgat
     5401 gaggaccagg accaggagag ggacacggtg ttcctgaagg acaaccacct ggccattgag
     5461 cgcaagtgct ccagcatcac ggtcagctct acgtctagcc tggaggctga ggtggacttc
     5521 acggtcattg gtgactacca tggcagcgcc ttcgaagact tctcccgcag cctgcctgag
     5581 ctcgaccggg acaaaagcga ctcggacact gagggcctgc tgttctcccg ggatctcaac
     5641 aagggggccc ccagccagga tgatgagtct gggggcattg aggacagccc ggatcgaggg
     5701 gcctgctcca ccccggatat gccccagttt gagcccgtga aaacagaaac catgactgtc
     5761 agcagtctgg ccattagaaa gaagattgag ccggaggccg tactgcagac cagagtctcc
     5821 gctatggata acacccaggt tgatgggagt gcctcagtgg ggagggagtt catagcaacc
     5881 actccctcca tcaccacgga gaccatatcg accaccatgg agaacagtct caagtccggg
     5941 aagggggcag ctgccatgat cccaggccca cagacggtgg ccacggaaat ccgttctctt
     6001 tctccgatca tcgggaaaga tgtcctcacc agcacctacg gcgccactgc ggaaaccctc
     6061 tcaacctcca ccaccaccca tgtcaccaaa actgtgaaag gagggttttc tgagacaagg
     6121 atcgagaagc gaatcatcat tactggggat gaagatgtcg atcaagacca ggccctggct
     6181 ttggccatca aggaggccaa actgcagcat cctgatatgc tggtaaccaa agctgtcgta
     6241 tacagagaaa cagacccatc cccagaggag agggacaaga agccacagga atcctaggac
     6301 ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gacaatcaac
     6361 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6421 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6481 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6541 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6601 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6661 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6721 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     6781 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6841 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6901 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt ctaccgggta
     6961 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     7021 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     7081 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     7141 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     7201 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     7261 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     7321 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     7381 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     7441 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
     7501 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     7561 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     7621 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7681 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     7741 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7801 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7861 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7921 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     7981 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     8041 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     8101 cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg
     8161 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     8221 ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     8281 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8341 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8401 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     8461 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     8521 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8581 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8641 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8701 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8761 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     8821 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     8881 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     8941 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     9001 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     9061 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     9121 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     9181 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     9241 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     9301 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     9361 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     9421 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     9481 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     9541 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     9601 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9661 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9721 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9781 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9841 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9901 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9961 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10021 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    10081 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    10141 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    10201 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    10261 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10321 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    10381 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10441 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    10501 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10561 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10621 aa
//
