LOCUS       sequence-205680-       11504 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation R462E with approximately
            wildtype activity, decreased surface expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTR462E-myc
FEATURES             Location/Qualifiers
     source          1..11504
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(773..791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        859..963
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(964..1032,1033..1824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1182..1201)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1995..2583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2484..2503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3014..3159
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3202..4239
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4237..4256)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4281..4329
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4983..5362
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5363..5566
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5517..5537
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5563..5587
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5615..5624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6290..6319
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7608..7742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7632..7651)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7686..7705
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7885..7907)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8467..10256
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct
       61 gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg
      121 ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt
      181 cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt
      241 ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg
      301 ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa
      361 tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc
      421 aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa
      481 ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc
      541 ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc
      601 cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag
      661 gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga
      721 tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg
      781 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      841 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      901 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      961 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1021 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1081 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1141 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1201 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1261 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1321 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1381 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1441 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1501 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1561 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1621 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1681 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1741 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1801 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1861 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1921 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1981 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2041 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2101 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2161 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2221 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2281 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2341 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2401 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2461 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2521 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2581 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt
     2641 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     2701 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     2761 agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg ctggagatgg
     2821 cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga
     2881 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cctgcttcat
     2941 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
     3001 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
     3061 gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg tgacgcgtgt
     3121 ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca
     3181 gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     3241 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     3301 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     3361 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     3421 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     3481 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     3541 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     3601 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     3661 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     3721 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     3781 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     3841 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     3901 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     3961 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     4021 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     4081 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     4141 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     4201 agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg agacaatacc
     4261 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
     4321 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     4381 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     4441 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg
     4501 gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat
     4561 tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt
     4621 tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg
     4681 gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt gccaagctag
     4741 tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt
     4801 gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt
     4861 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
     4921 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg
     4981 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     5041 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     5101 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     5161 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     5221 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
     5281 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
     5341 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
     5401 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     5461 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
     5521 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     5581 tcagatctct agaagctggg taccagctgc tagcgccacc atggagacca cccccctgaa
     5641 cagtcaaaag caactctctg cctgtgagga tggagaggac tgtcaggaga atggcgttct
     5701 tcaaaaggtt gtacccaccc ctggagataa ggtggaaagc ggccagattt ccaacggcta
     5761 cagcgccgtg ccatcacctg gagccgggga tgatacacgg cacagcattc ccgcaaccac
     5821 cacgacactg gttgcagagc ttcaccaggg cgagagggag acgtggggca aaaaggtaga
     5881 ttttctgctt tccgtgatcg gttacgcggt cgatctgggg aacgtgtgga ggttccctta
     5941 catttgttac caaaacggag ggggtgcctt cttgctgccc tacacaatta tggcgatttt
     6001 tgggggtatc ccgctcttct acatggaact ggccttgggt cagtaccacc ggaacggctg
     6061 cattagcatt tggcgcaaga tatgccccat tttcaaggga atcggatacg caatctgtat
     6121 aatcgcgttc tatattgcat catattacaa caccatcatg gcctgggccc tttattactt
     6181 gatcagcagt ttcaccgacc aattgccgtg gacgagctgt aagaactcat ggaataccgg
     6241 gaactgtacc aactacttct ctgaggacag ctcaggaggt agcgggggtg aacagaagct
     6301 gattagcgag gaggacttgg gcagtccagg cggaagcggc aacatcactt ggacactcca
     6361 tagtacgagc cccgcagagg agttttacac caggcacgta ctgcagattc ataggagcaa
     6421 gggtctgcaa gacctgggcg gtattagctg gcagctcgca ttgtgcataa tgttgatctt
     6481 tacggtgatc tatttctcta tctggaaggg agtaaagaca tccggcaagg tggtgtgggt
     6541 gacggcgacc ttcccctaca ttatactcag cgtgttgttg gtgagggggg caaccctgcc
     6601 tggggcctgg cgaggcgtgc tgttctatct caagccaaac tggcagaagc ttcttgaaac
     6661 cggagtgtgg atcgacgcgg ccgcgcaaat cttcttttcc ctcggtcccg gcttcggggt
     6721 ccttctggca ttcgcaagct acaacaagtt caacaataac tgctaccagg acgccctcgt
     6781 gacaagtgta gtgaactgca tgacctcttt cgtgagcggg tttgtgatct tcacagtact
     6841 cggatatatg gctgagatga ggaacgaaga tgttagtgag gttgccaaag acgctggccc
     6901 gagcctgttg ttcatcacat acgccgaggc tatcgcgaac atgcccgcat ctactttctt
     6961 cgccatcatc tttttcctga tgctgataac ccttgggctg gatagcacct tcgccggact
     7021 ggagggtgtc atcactgccg ttctcgacga gttcccccat gtgtgggcca agcgagagga
     7081 aaggttcgtg ctggccgtcg taatcacatg cttcttcggc tcactggtga cccttacctt
     7141 tggcggtgcg tacgtggtca agctgctcga agagtacgcc actggaccgg cggttctgac
     7201 cgtggcattg atcgaggccg tggccgtaag ctggttttac ggcatcacgc agttctgtag
     7261 ggacgtgaaa gagatgctgg gcttttctcc cggttggttt tggaggattt gttgggtagc
     7321 aattagcccc ctgtttctcc ttttcattat ttgctctttt ctgatgtctc caccgcagct
     7381 ccgactgttt cagtacaact acccctactg gtcaattatc ctgggctatt gtatcggcac
     7441 ctcaagtttc atctgcatcc ccacatacat cgcatacaga ctgataatta cgcccggtac
     7501 ttttaaggag cgaatcatta aaagcataac ccccgaaacc cccaccgaga tcccttgtgg
     7561 cgatataaga ctcaatgcgg tctaatagct cgaggccggc aaggccggat ccagacatga
     7621 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     7681 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     7741 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     7801 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg gctgcctcgc
     7861 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     7921 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     7981 cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc cccggacgaa
     8041 ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt aatcccttca
     8101 gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa
     8161 acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca
     8221 acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt
     8281 gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc
     8341 caggggccca ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc
     8401 taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta
     8461 accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta
     8521 attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag
     8581 ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg
     8641 gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa
     8701 ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct
     8761 aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt
     8821 ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat
     8881 gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca
     8941 ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg
     9001 tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg
     9061 gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct
     9121 atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct
     9181 aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg
     9241 gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct
     9301 gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca
     9361 ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa
     9421 gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac
     9481 acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga
     9541 gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat
     9601 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     9661 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     9721 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     9781 gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat
     9841 gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta
     9901 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta
     9961 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
    10021 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
    10081 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
    10141 tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt
    10201 gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg
    10261 ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg
    10321 ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga
    10381 gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca
    10441 agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt
    10501 agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca
    10561 ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc
    10621 accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc
    10681 gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag
    10741 gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa
    10801 ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc
    10861 aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg
    10921 ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt
    10981 tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc
    11041 aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa accaccctcc
    11101 ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc cttctcctgg
    11161 gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac catctgggcc
    11221 accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa atggccttct
    11281 acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac tgggactcct
    11341 gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac ttccccccct
    11401 ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc ggcctccact
    11461 acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctc
//
