LOCUS       sequence-20569-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(36..160)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(168..184)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(168..184)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(199..640)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(205..228)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    523..640
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(664..684)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(664..682)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(703..719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(703..719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaagtgtaaa gcctgggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt
       61 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      121 cattttacgt ttctcgttca gcttttttgt acaaacttgt gatgggggat ccactagttc
      181 tagaatccgg ggttttttct ccttgacgtt aaagtataga ggtatattaa caattttttg
      241 ttgatacttt tattacattt gaataagaag taatacaaac cgaaaatgtt gaaagtatta
      301 gttaaagtgg ttatgcagtt tttgcattta tatatctgtt aatagatcaa aaatcatcgc
      361 ttcgctgatt aattacccca gaaataaggc taaaaaacta atcgcattat catcctatgg
      421 ttgttaattt gattcgttca tttgaaggtt tgtggggcca ggttactgcc aatttttcct
      481 cttcataacc ataaaagcta gtattgtaga atctttattg ttcggagcag tgcggcgcga
      541 ggcacatctg cgtttcagga acgcgaccgg tgaagacgag gacgcacgga ggagagtctt
      601 ccttcggagg gctgtcaccc gctcggcggc ttctaatccg tactagagct ccagcttttg
      661 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
      721 g
//
