LOCUS       sequence-205693-        8606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing CRIP2, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) CRIP2
FEATURES             Location/Qualifiers
     source          1..8606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     517..536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     770..787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    904..925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(940..945,946..963,964..970)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    978..994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     983..1005
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1002..1018
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1002..1018
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1037..1057
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1039..1057
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1085..1311
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1312..1492
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1539..1664
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2157..2390
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2575..2619
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2886..3002
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3025..3328
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3329..3532
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3483..3503
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3529..3553
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3637..3661
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4295..4319)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4350..4938
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4403..4423)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4821..4832)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4952..5451
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4987..5010)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5384..5403
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5472..6071
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5472..5491)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5968..5988
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6209..6442
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6514..6635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6551..6570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6605..6624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6675..6810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6830..6849)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6831..6849)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6859..6876)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6859..6875)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6868..6890)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7017..7472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7104..7123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7314..7335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7498..7602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7603..7671,7672..8463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7821..7840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
       61 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      121 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      181 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      241 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      301 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      421 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      481 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      721 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      781 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      841 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      901 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      961 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1021 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     1081 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     1141 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     1201 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     1261 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     1321 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     1381 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     1441 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1501 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1561 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1621 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1681 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1741 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1801 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1861 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1921 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1981 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     2041 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     2101 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     2161 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     2221 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     2281 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     2341 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     2401 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     2461 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2521 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2581 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2641 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2701 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2761 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2821 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     2881 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2941 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt
     3001 ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     3061 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3121 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3181 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3241 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3301 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3361 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     3421 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     3481 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     3541 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     3601 actctagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa aaaagcaggc
     3661 ttcaccatgg cttcgaaatg cccaaaatgc gacaagaccg tatattttgc ggaaaaggtc
     3721 tctagtctgg gtaaagattg gcacaaattc tgcctcaagt gcgaacgttg ctcaaagacc
     3781 ctgacacctg gtggccatgc tgagcacgat ggtaaaccct tttgtcataa gccttgttat
     3841 gcaacccttt ttggccccaa gggggtgaac attggtgggg ccgggtccta catatacgag
     3901 aagccattgg ccgagggacc gcaagttacc gggcccattg aggtccccgc agcccgggca
     3961 gaggaacgga aggccagcgg gccgcctaag ggcccatctc gcgcctcctc agtgaccaca
     4021 ttcactgggg aacctaatac atgtcctcga tgtagtaaaa aggtttactt cgccgaaaaa
     4081 gtcacgagct taggaaagga ttggcataga ccttgcctaa ggtgtgagag gtgcggaaaa
     4141 acactcactc ccggaggaca cgctgagcac gacggccagc catactgcca caagccctgt
     4201 tacggcatcc tgttcggccc caaaggagtg aacactggcg ctgtgggcag ctatatctat
     4261 gacagagacc cagagggcaa agtgcagcca tgaaacccag ctttcttgta caaagtggtt
     4321 gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa
     4381 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4441 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4501 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4561 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4621 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4681 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4741 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4801 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4861 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4921 tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag
     4981 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     5041 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     5101 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     5161 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     5221 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     5281 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     5341 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     5401 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     5461 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5521 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5581 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5641 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5701 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     5761 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5821 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     5881 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     5941 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6001 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6061 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     6121 atgcatctcg agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6181 agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa
     6241 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6301 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6361 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6421 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6481 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6541 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6601 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6661 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6781 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     6841 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6901 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     6961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     7021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7081 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7201 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7321 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7381 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7441 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     7501 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7561 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7621 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7681 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7741 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7801 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7861 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7921 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7981 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8041 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8101 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8161 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8221 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8281 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8341 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8401 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8461 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8521 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8581 gagttttcgt tccactgagc gtcaga
//
