LOCUS       sequence-205700-       11468 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation K490L conferring gain of
            function phenotype, decreased surface expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTK490L-myc
FEATURES             Location/Qualifiers
     source          1..11468
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(720..738)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        806..910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(911..979,980..1771)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1129..1148)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1942..2530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2431..2450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2961..3106
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3149..4186
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4184..4203)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4228..4276
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4930..5309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5310..5513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5464..5484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5510..5534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5562..5571
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6237..6266
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7555..7689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7579..7598)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7633..7652
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7832..7854)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8414..10203
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc aacttggacg
       61 tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc
      121 tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc
      181 ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc
      241 tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag
      301 agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc
      361 cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt
      421 ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg
      481 aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt
      541 cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct
      601 cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct
      661 gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg
      721 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      901 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      961 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1021 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1081 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1141 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1201 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1261 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1321 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1381 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1441 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1501 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1561 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1621 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1681 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1741 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1801 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1861 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg
     2581 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     2641 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     2701 gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg
     2761 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     2821 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct
     2881 gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt
     2941 tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt
     3001 cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga
     3061 cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg
     3121 tgccgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt
     3181 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     3241 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     3301 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     3361 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     3421 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     3481 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     3541 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     3601 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     3661 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     3721 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     3781 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     3841 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     3901 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     3961 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     4021 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     4081 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     4141 acgccccagc actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga
     4201 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
     4261 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     4321 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     4381 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     4441 gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg
     4501 gggttattat tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc
     4561 catggttttt ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc
     4621 gtctgtggct gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc
     4681 aagctagtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
     4741 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
     4801 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
     4861 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
     4921 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     4981 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     5041 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     5101 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     5161 cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat
     5221 ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca
     5281 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc
     5341 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     5401 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt
     5461 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     5521 tgaaccgtca gatctctaga agctgggtac cagctgctag cgccaccatg gagaccaccc
     5581 ccctgaacag tcaaaagcaa ctctctgcct gtgaggatgg agaggactgt caggagaatg
     5641 gcgttcttca aaaggttgta cccacccctg gagataaggt ggaaagcggc cagatttcca
     5701 acggctacag cgccgtgcca tcacctggag ccggggatga tacacggcac agcattcccg
     5761 caaccaccac gacactggtt gcagagcttc accagggcga gagggagacg tggggcaaaa
     5821 aggtagattt tctgctttcc gtgatcggtt acgcggtcga tctggggaac gtgtggaggt
     5881 tcccttacat ttgttaccaa aacggagggg gtgccttctt gctgccctac acaattatgg
     5941 cgatttttgg gggtatcccg ctcttctaca tggaactggc cttgggtcag taccaccgga
     6001 acggctgcat tagcatttgg cgcaagatat gccccatttt caagggaatc ggatacgcaa
     6061 tctgtataat cgcgttctat attgcatcat attacaacac catcatggcc tgggcccttt
     6121 attacttgat cagcagtttc accgaccaat tgccgtggac gagctgtaag aactcatgga
     6181 ataccgggaa ctgtaccaac tacttctctg aggacagctc aggaggtagc gggggtgaac
     6241 agaagctgat tagcgaggag gacttgggca gtccaggcgg aagcggcaac atcacttgga
     6301 cactccatag tacgagcccc gcagaggagt tttacaccag gcacgtactg cagattcata
     6361 ggagcaaggg tctgcaagac ctgggcggta ttagctggca gctcgcattg tgcataatgt
     6421 tgatctttac ggtgatctat ttctctatct ggaagggagt aaagacatcc ggcaaggtgg
     6481 tgtgggtgac ggcgaccttc ccctacatta tactcagcgt gttgttggtg aggggggcaa
     6541 ccctgcctgg ggcctggcga ggcgtgctgt tctatctcaa gccaaactgg cagaagcttc
     6601 ttgaaaccgg agtgtggatc gacgcggccg cgcaaatctt cttttccctc ggtcccggct
     6661 tcggggtcct tctggcattc gcaagctaca acaagttcaa caataactgc taccaggacg
     6721 ccctcgtgac aagtgtagtg aactgcatga cctctttcgt gagcgggttt gtgatcttca
     6781 cagtactcgg atatatggct gagatgagga acgaagatgt tagtgaggtt gccaaagacg
     6841 ctggcccgag cctgttgttc atcacatacg ccgaggctat cgcgaacatg cccgcatcta
     6901 ctttcttcgc catcatcttt ttcctgatgc tgataaccct tgggctggat agcaccttcg
     6961 ccggactgga gggtgtcatc actgccgttc tcgacgagtt cccccatgtg tgggccaagc
     7021 gacgggaaag gttcgtgctg gccgtcgtaa tcacatgctt cttcggctca ctggtgaccc
     7081 ttacctttgg cggtgcgtac gtggtcctgc tgctcgaaga gtacgccact ggaccggcgg
     7141 ttctgaccgt ggcattgatc gaggccgtgg ccgtaagctg gttttacggc atcacgcagt
     7201 tctgtaggga cgtgaaagag atgctgggct tttctcccgg ttggttttgg aggatttgtt
     7261 gggtagcaat tagccccctg tttctccttt tcattatttg ctcttttctg atgtctccac
     7321 cgcagctccg actgtttcag tacaactacc cctactggtc aattatcctg ggctattgta
     7381 tcggcacctc aagtttcatc tgcatcccca catacatcgc atacagactg ataattacgc
     7441 ccggtacttt taaggagcga atcattaaaa gcataacccc cgaaaccccc accgagatcc
     7501 cttgtggcga tataagactc aatgcggtct aatagctcga ggccggcaag gccggatcca
     7561 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     7621 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     7681 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     7741 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatccggct
     7801 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     7861 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     7921 gtgttggcgg gtgtcggggc gcagccatga ggtcgactct agaggatcga tccccgcccc
     7981 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     8041 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     8101 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     8161 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     8221 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     8281 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     8341 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     8401 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     8461 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     8521 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     8581 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     8641 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     8701 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     8761 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     8821 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     8881 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     8941 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     9001 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     9061 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     9121 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     9181 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     9241 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     9301 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     9361 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     9421 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     9481 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     9541 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     9601 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     9661 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     9721 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     9781 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     9841 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     9901 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     9961 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
    10021 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
    10081 tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag tgggagtgct
    10141 atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct tgtccttttc
    10201 ctgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt
    10261 ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg
    10321 gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga ggacgaaaat
    10381 ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta ctggggattt
    10441 attctttagt gcgggggaat acacggcttt taatacgatt gagggcgtct cctaacaagt
    10501 tacatcactc ctgcccttcc tcaccctcat ctccatcacc tccttcatct ccgtcatctc
    10561 cgtcatcacc ctccgcggca gccccttcca ccataggtgg aaaccaggga ggcaaatcta
    10621 ctccatcgtc aaagctgcac acagtcaccc tgatattgca ggtaggagcg ggctttgtca
    10681 taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga
    10741 ccatgaaata acagacaatg gactccctta gcgggccagg ttgtgggccg ggtccagggg
    10801 ccattccaaa ggggagacga ctcaatggtg taagacgaca ttgtggaata gcaagggcag
    10861 ttcctcgcct taggttgtaa agggaggtct tactacctcc atatacgaac acaccggcga
    10921 cccaagttcc ttcgtcggta gtcctttcta cgtgactcct agccaggaga gctcttaaac
    10981 cttctgcaat gttctcaaat ttcgggttgg aacctccttg accacgatgc tttccaaacc
    11041 accctccttt tttgcgcctg cctccatcac cctgaccccg gggtccagtg cttgggcctt
    11101 ctcctgggtc atctgcgggg ccctgctcta tcgctcccgg gggcacgtca ggctcaccat
    11161 ctgggccacc ttcttggtgg tattcaaaat aatcggcttc ccctacaggg tggaaaaatg
    11221 gccttctacc tggagggggc ctgcgcggtg gagacccgga tgatgatgac tgactactgg
    11281 gactcctggg cctcttttct ccacgtccac gacctctccc cctggctctt tcacgacttc
    11341 cccccctggc tctttcacgt cctctacccc ggcggcctcc actacctcct cgaccccggc
    11401 ctccactacc tcctcgaccc cggcctccac tgcctcctcg accccggcct ccacctcctg
    11461 ctcctgcc
//
