LOCUS       sequence-205713-       11496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation F556H conferring gain of
            function phenotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTF556H-myc
FEATURES             Location/Qualifiers
     source          1..11496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(709..727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        795..899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(900..968,969..1760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1118..1137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1931..2519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2420..2439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2950..3095
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3138..4175
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4173..4192)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4217..4265
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4919..5298
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5299..5502
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5453..5473
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5499..5523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5551..5560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6226..6255
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7544..7678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7568..7587)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7622..7641
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7821..7843)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8403..10192
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctgctcctgt tccaccgtgg gtccctttgc agccaatgca acttggacgt ttttggggtc
       61 tccggacacc atctctatgt cttggccctg atcctgagcc gcccggggct cctggtcttc
      121 cgcctcctcg tcctcgtcct cttccccgtc ctcgtccatg gttatcaccc cctcttcttt
      181 gaggtccact gccgccggag ccttctggtc cagatgtgtc tcccttctct cctaggccat
      241 ttccaggtcc tgtacctggc ccctcgtcag acatgattca cactaaaaga gatcaataga
      301 catctttatt agacgacgct cagtgaatac agggagtgca gactcctgcc ccctccaaca
      361 gcccccccac cctcatcccc ttcatggtcg ctgtcagaca gatccaggtc tgaaaattcc
      421 ccatcctccg aaccatcctc gtcctcatca ccaattactc gcagcccgga aaactcccgc
      481 tgaacatcct caagatttgc gtcctgagcc tcaagccagg cctcaaattc ctcgtccccc
      541 tttttgctgg acggtaggga tggggattct cgggacccct cctcttcctc ttcaaggtca
      601 ccagacagag atgctactgg ggcaacggaa gaaaagctgg gtgcggcctg tgaggatcag
      661 cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac
      721 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      781 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      841 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      901 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      961 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1021 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1081 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1141 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1201 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1261 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1321 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1381 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1441 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1501 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1561 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1621 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1681 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1741 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1801 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1861 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1921 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1981 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2041 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     2101 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2161 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2221 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2281 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2341 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2401 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2461 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2521 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggccttga agctgtccct
     2581 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc
     2641 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca
     2701 agacgtagcc cagcgcgtcg gccccgagat gcgccgcgtg cggctgctgg agatggcgga
     2761 cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg
     2821 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccctg cttcatcccc
     2881 gtggcccgtt gctcgcgttt gctggcggtg tccccggaag aaatatattt gcatgtcttt
     2941 agttctatga tgacacaaac cccgcccagc gtcttgtcat tggcgaattc gaacacgcag
     3001 atgcagtcgg ggcggcgcgg tccgaggtcc acttcgcata ttaaggtgac gcgtgtggcc
     3061 tcgaacaccg agcgaccctg cagcgacccg cttaacagcg tcaacagcgt gccgcagatc
     3121 ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     3181 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     3241 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     3301 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     3361 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     3421 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     3481 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     3541 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     3601 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     3661 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     3721 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     3781 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     3841 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     3901 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     3961 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     4021 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     4081 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     4141 ctcgtccgga tcgggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa
     4201 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     4261 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     4321 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     4381 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc
     4441 cgtgggttag ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt
     4501 ttgggcgttg cgtggggtca ggtccacgac tggactgagc agacagaccc atggtttttg
     4561 gatggcctgg gcatggaccg catgtactgg cgcgacacga acaccgggcg tctgtggctg
     4621 ccaaacaccc ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca agctagtcga
     4681 ccaattctca tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg
     4741 caggcgcaga actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc
     4801 atattagtca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
     4861 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttga
     4921 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4981 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     5041 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     5101 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     5161 gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     5221 cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta
     5281 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg
     5341 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     5401 caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg
     5461 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     5521 atctctagaa gctgggtacc agctgctagc gccaccatgg agaccacccc cctgaacagt
     5581 caaaagcaac tctctgcctg tgaggatgga gaggactgtc aggagaatgg cgttcttcaa
     5641 aaggttgtac ccacccctgg agataaggtg gaaagcggcc agatttccaa cggctacagc
     5701 gccgtgccat cacctggagc cggggatgat acacggcaca gcattcccgc aaccaccacg
     5761 acactggttg cagagcttca ccagggcgag agggagacgt ggggcaaaaa ggtagatttt
     5821 ctgctttccg tgatcggtta cgcggtcgat ctggggaacg tgtggaggtt cccttacatt
     5881 tgttaccaaa acggaggggg tgccttcttg ctgccctaca caattatggc gatttttggg
     5941 ggtatcccgc tcttctacat ggaactggcc ttgggtcagt accaccggaa cggctgcatt
     6001 agcatttggc gcaagatatg ccccattttc aagggaatcg gatacgcaat ctgtataatc
     6061 gcgttctata ttgcatcata ttacaacacc atcatggcct gggcccttta ttacttgatc
     6121 agcagtttca ccgaccaatt gccgtggacg agctgtaaga actcatggaa taccgggaac
     6181 tgtaccaact acttctctga ggacagctca ggaggtagcg ggggtgaaca gaagctgatt
     6241 agcgaggagg acttgggcag tccaggcgga agcggcaaca tcacttggac actccatagt
     6301 acgagccccg cagaggagtt ttacaccagg cacgtactgc agattcatag gagcaagggt
     6361 ctgcaagacc tgggcggtat tagctggcag ctcgcattgt gcataatgtt gatctttacg
     6421 gtgatctatt tctctatctg gaagggagta aagacatccg gcaaggtggt gtgggtgacg
     6481 gcgaccttcc cctacattat actcagcgtg ttgttggtga ggggggcaac cctgcctggg
     6541 gcctggcgag gcgtgctgtt ctatctcaag ccaaactggc agaagcttct tgaaaccgga
     6601 gtgtggatcg acgcggccgc gcaaatcttc ttttccctcg gtcccggctt cggggtcctt
     6661 ctggcattcg caagctacaa caagttcaac aataactgct accaggacgc cctcgtgaca
     6721 agtgtagtga actgcatgac ctctttcgtg agcgggtttg tgatcttcac agtactcgga
     6781 tatatggctg agatgaggaa cgaagatgtt agtgaggttg ccaaagacgc tggcccgagc
     6841 ctgttgttca tcacatacgc cgaggctatc gcgaacatgc ccgcatctac tttcttcgcc
     6901 atcatctttt tcctgatgct gataaccctt gggctggata gcaccttcgc cggactggag
     6961 ggtgtcatca ctgccgttct cgacgagttc ccccatgtgt gggccaagcg acgggaaagg
     7021 ttcgtgctgg ccgtcgtaat cacatgcttc ttcggctcac tggtgaccct tacctttggc
     7081 ggtgcgtacg tggtcaagct gctcgaagag tacgccactg gaccggcggt tctgaccgtg
     7141 gcattgatcg aggccgtggc cgtaagctgg ttttacggca tcacgcagtt ctgtagggac
     7201 gtgaaagaga tgctgggctt ttctcccggt tggttttgga ggatttgttg ggtagcaatt
     7261 agccccctgt ttctcctttt cattatttgc tctcatctga tgtctccacc gcagctccga
     7321 ctgtttcagt acaactaccc ctactggtca attatcctgg gctattgtat cggcacctca
     7381 agtttcatct gcatccccac atacatcgca tacagactga taattacgcc cggtactttt
     7441 aaggagcgaa tcattaaaag cataaccccc gaaaccccca ccgagatccc ttgtggcgat
     7501 ataagactca atgcggtcta atagctcgag gccggcaagg ccggatccag acatgataag
     7561 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     7621 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     7681 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     7741 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggctg cctcgcgcgt
     7801 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     7861 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7921 tgtcggggcg cagccatgag gtcgactcta gaggatcgat ccccgccccg gacgaactaa
     7981 acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg
     8041 gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac
     8101 aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac
     8161 tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct
     8221 ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg
     8281 ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc
     8341 gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc
     8401 taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta accctaattc
     8461 aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc
     8521 ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca
     8581 ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag
     8641 cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata
     8701 gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca
     8761 ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca
     8821 ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct
     8881 gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca
     8941 tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt
     9001 tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt
     9061 gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca
     9121 cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca
     9181 ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg
     9241 ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc
     9301 ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag
     9361 gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt
     9421 gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac
     9481 ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta
     9541 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     9601 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     9661 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     9721 tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta
     9781 tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     9841 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     9901 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     9961 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
    10021 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
    10081 catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta tcctttgcat
    10141 atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc
    10201 tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca
    10261 ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga
    10321 cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg gtgacaagac
    10381 agatggccag aaatacacca acagcacgca tgatgtctac tggggattta ttctttagtg
    10441 cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt acatcactcc
    10501 tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc gtcatcaccc
    10561 tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac tccatcgtca
    10621 aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat aacaaggtcc
    10681 ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac catgaaataa
    10741 cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc cattccaaag
    10801 gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt tcctcgcctt
    10861 aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac ccaagttcct
    10921 tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc ttctgcaatg
    10981 ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca ccctcctttt
    11041 ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca
    11101 tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc tgggccacct
    11161 tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg ccttctacct
    11221 ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg actcctgggc
    11281 ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc ccccctggct
    11341 ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc tccactacct
    11401 cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc tcctgcccct
    11461 cctgctcctg cccctcctcc tgctcctgcc cctcct
//
