LOCUS       sequence-205716-        4780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  partial cDNA of a neurotrophin responsive gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Levi A, Eldridge JD, Paterson BM
  TITLE     Molecular cloning of a gene sequence regulated by nerve growth 
            factor.
  JOURNAL   Science. 1985 Jul 26;229(4711):393-5.
  PUBMED    3839317
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1985-07-26"; volume: "229(4711)"; pages: "393-5"
COMMENT     Sequence Label: VGF8a
FEATURES             Location/Qualifiers
     source          1..4780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      15..603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     504..523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     757..774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    891..912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(927..932,933..950,951..957)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    965..981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     970..992
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     989..1005
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     989..1005
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3169..3186)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3169..3185)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3178..3200)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3394..3413)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3513..3535
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3573..3591)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3659..3763
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3764..3832,3833..4624)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3982..4001)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
       61 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      121 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      781 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      841 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
      901 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
      961 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgaattc
     1021 cgagcctcag gaccagggag agctcttcca gggcgtggat ccccgggcgc tggccgcggt
     1081 actgttgcag gcactggacc gtccggcctc gcccccggct gtcccggcag gttcccagca
     1141 gggaacaccc gaagaagcag cagaagctct gctgaccgag tccgtgcgca gtcagaccca
     1201 tagcctcccg gcatcagaaa tccaagcgtc cgctgtggcg ccccctcgcc ctcagactca
     1261 ggacaacgat cccgaggcag acgaccgctc agaagagctg gaggcactag catccttgct
     1321 ccaagaactt cgagatttca gtccgagtaa tgctaagcgc cagcaagaga cggcggcagc
     1381 agagactgaa acccgcacgc acacgctgac ccgagtcaat ctggagagcc ccgggccaga
     1441 gcgcgtatgg cgcgcttcct ggggagagtt ccaggcgcgc gtcccggagc gtgctcctct
     1501 gccgccctcg gtcccttctc aattccaggc tcgaatgtcc gaaaacgttc cccttcccga
     1561 aacccatcag ttcggggaag gagtgtcctc ccctaaaaca catcttggtg agactttgac
     1621 acccttatcc aaggcgtacc aaagtctaag tgcccccttc cccaaggtgc gtcggctcga
     1681 gggctcattc ctgggcggtt ccgaggcagg agagcgcctg cttcaacaag ggttagctca
     1741 ggtagaggca gggaggaggc aggcggaggc cacccggcag gccgcagcgc aagaagagcg
     1801 gctggccgat ctcgcctccg acctgctgct ccagtatttg ctgcagggcg gcgcccggca
     1861 gcgcgatctc gggggtcgcg ggctgcagga gacgcagcaa gagcgggaga acgagaggga
     1921 ggaggaggcg gagcaggaga gacgcggtgg tggggaggac gaggtggggg aagaggatga
     1981 ggaggcggca gaggcggagg cggaggcaga ggaggcggag agggcgcggc agaacgcgct
     2041 cctgttcgcc gaggaggagg acggggaagc cggagccgag gacaagcgct cccaggagga
     2101 ggcgccaggc catcggcgga aggatgctga ggggacagag gagggcgggg aggaggatga
     2161 cgacgacgaa gagatggatc cgcagacgat cgatagtctc attgaactgt ccaccaaact
     2221 ccacctgcca gcagacgatg tggtcagcat catcgaagag atggaggaga aacggaagcg
     2281 gaagaagaac gcccctcccg agccggtgcc gccccccagg gctgccccag ccccgaccca
     2341 tgtccgctcc ccgcagcccc cacctcccgc cccggcccgg gatgagttgc cggactggaa
     2401 cgaagtactc ccaccctggg atcgggagga ggatgaggtg tttcccccgg ggccctatca
     2461 ccccttccca aactacattc ggccgcggac actgcagccg cccgcatcct cccgccgccg
     2521 tcacttccat cacgcgttgc cacctgcgcg ccaccatccc gatctggagg cccaggccag
     2581 gcgcgcgcag gaggaagcgg acgcggagga gcgccggctg caggagcagg aggagctgga
     2641 gaattacatt gagcacgtgc tgctgcaccg cccgtgaccc gcccctgcgc gcccgctccc
     2701 aactgcgcgc gccgccacgc ccccctccgt gtcgctcctc ctccctctcg gtgtttgcat
     2761 gcgccccggc tccgcccctc ggctgccgcc cggccccgcc ccacaaggcc ccgccccggg
     2821 ttctgtcagg accagacctg tcagacttct ttggggtctg atcctggggc cagcccaggc
     2881 gggtgtgtgg tttgtgcgag tccccttaca cccccacttc ctccaggggc ctcgtcccca
     2941 tctagtttct ctagcgactt cctggtccca aacggggaaa agctgttcta tttaatcgtg
     3001 tgaagtgtct gtctcccagc cttggggccc ccggagcctc ccttctccaa attgctgtga
     3061 acttacccac atcttgccct tctgttgtaa atacccctca cggaggaaat agttttgcta
     3121 agaaataaaa gtgactattt taaaaaaaaa aaaaaaccca agcttggcac tggccgtcgt
     3181 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3241 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3301 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     3361 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3421 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     3481 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3541 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3601 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3661 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3721 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3781 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3841 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3901 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3961 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4021 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4081 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4141 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4201 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4261 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4321 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4381 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4441 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4501 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4561 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4621 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4681 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4741 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
//
