LOCUS       sequence-205717-       11477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation I576K with approximately
            wildtype activity, decreased surface expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTI576K-myc
FEATURES             Location/Qualifiers
     source          1..11477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(706..724)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        792..896
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(897..965,966..1757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1115..1134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1928..2516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2417..2436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2947..3092
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3135..4172
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4170..4189)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4214..4262
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4916..5295
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5296..5499
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5450..5470
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5496..5520
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5548..5557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6223..6252
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7541..7675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7565..7584)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7619..7638
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7818..7840)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8400..10189
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctcctgttcc accgtgggtc cctttgcagc caatgcaact tggacgtttt tggggtctcc
       61 ggacaccatc tctatgtctt ggccctgatc ctgagccgcc cggggctcct ggtcttccgc
      121 ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt atcaccccct cttctttgag
      181 gtccactgcc gccggagcct tctggtccag atgtgtctcc cttctctcct aggccatttc
      241 caggtcctgt acctggcccc tcgtcagaca tgattcacac taaaagagat caatagacat
      301 ctttattaga cgacgctcag tgaatacagg gagtgcagac tcctgccccc tccaacagcc
      361 cccccaccct catccccttc atggtcgctg tcagacagat ccaggtctga aaattcccca
      421 tcctccgaac catcctcgtc ctcatcacca attactcgca gcccggaaaa ctcccgctga
      481 acatcctcaa gatttgcgtc ctgagcctca agccaggcct caaattcctc gtcccccttt
      541 ttgctggacg gtagggatgg ggattctcgg gacccctcct cttcctcttc aaggtcacca
      601 gacagagatg ctactggggc aacggaagaa aagctgggtg cggcctgtga ggatcagctt
      661 atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc
      721 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      781 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      841 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      901 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      961 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1021 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1081 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     1141 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1201 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1261 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1321 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1381 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1441 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1501 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1561 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1621 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1681 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1741 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1801 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1861 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1921 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1981 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2041 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2101 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2161 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2221 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2281 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2341 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2401 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2461 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2521 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttgaagc tgtccctgat
     2581 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg
     2641 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga
     2701 cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc
     2761 gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt
     2821 ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccctgctt catccccgtg
     2881 gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt
     2941 tctatgatga cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg
     3001 cagtcggggc ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg tgtggcctcg
     3061 aacaccgagc gaccctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatcccg
     3121 gggggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     3181 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     3241 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     3301 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     3361 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     3421 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     3481 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     3541 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     3601 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     3661 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     3721 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     3781 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     3841 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     3901 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     3961 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     4021 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     4081 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     4141 gtccggatcg ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga
     4201 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     4261 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     4321 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     4381 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcca ctggccccgt
     4441 gggttaggga cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg
     4501 ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga cagacccatg gtttttggat
     4561 ggcctgggca tggaccgcat gtactggcgc gacacgaaca ccgggcgtct gtggctgcca
     4621 aacacccccg acccccaaaa accaccgcgc ggatttctgg cgtgccaagc tagtcgacca
     4681 attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag
     4741 gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca attagccata
     4801 ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
     4861 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat
     4921 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     4981 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     5041 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     5101 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     5161 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     5221 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
     5281 atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt
     5341 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     5401 caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc
     5461 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     5521 tctagaagct gggtaccagc tgctagcgcc accatggaga ccacccccct gaacagtcaa
     5581 aagcaactct ctgcctgtga ggatggagag gactgtcagg agaatggcgt tcttcaaaag
     5641 gttgtaccca cccctggaga taaggtggaa agcggccaga tttccaacgg ctacagcgcc
     5701 gtgccatcac ctggagccgg ggatgataca cggcacagca ttcccgcaac caccacgaca
     5761 ctggttgcag agcttcacca gggcgagagg gagacgtggg gcaaaaaggt agattttctg
     5821 ctttccgtga tcggttacgc ggtcgatctg gggaacgtgt ggaggttccc ttacatttgt
     5881 taccaaaacg gagggggtgc cttcttgctg ccctacacaa ttatggcgat ttttgggggt
     5941 atcccgctct tctacatgga actggccttg ggtcagtacc accggaacgg ctgcattagc
     6001 atttggcgca agatatgccc cattttcaag ggaatcggat acgcaatctg tataatcgcg
     6061 ttctatattg catcatatta caacaccatc atggcctggg ccctttatta cttgatcagc
     6121 agtttcaccg accaattgcc gtggacgagc tgtaagaact catggaatac cgggaactgt
     6181 accaactact tctctgagga cagctcagga ggtagcgggg gtgaacagaa gctgattagc
     6241 gaggaggact tgggcagtcc aggcggaagc ggcaacatca cttggacact ccatagtacg
     6301 agccccgcag aggagtttta caccaggcac gtactgcaga ttcataggag caagggtctg
     6361 caagacctgg gcggtattag ctggcagctc gcattgtgca taatgttgat ctttacggtg
     6421 atctatttct ctatctggaa gggagtaaag acatccggca aggtggtgtg ggtgacggcg
     6481 accttcccct acattatact cagcgtgttg ttggtgaggg gggcaaccct gcctggggcc
     6541 tggcgaggcg tgctgttcta tctcaagcca aactggcaga agcttcttga aaccggagtg
     6601 tggatcgacg cggccgcgca aatcttcttt tccctcggtc ccggcttcgg ggtccttctg
     6661 gcattcgcaa gctacaacaa gttcaacaat aactgctacc aggacgccct cgtgacaagt
     6721 gtagtgaact gcatgacctc tttcgtgagc gggtttgtga tcttcacagt actcggatat
     6781 atggctgaga tgaggaacga agatgttagt gaggttgcca aagacgctgg cccgagcctg
     6841 ttgttcatca catacgccga ggctatcgcg aacatgcccg catctacttt cttcgccatc
     6901 atctttttcc tgatgctgat aacccttggg ctggatagca ccttcgccgg actggagggt
     6961 gtcatcactg ccgttctcga cgagttcccc catgtgtggg ccaagcgacg ggaaaggttc
     7021 gtgctggccg tcgtaatcac atgcttcttc ggctcactgg tgacccttac ctttggcggt
     7081 gcgtacgtgg tcaagctgct cgaagagtac gccactggac cggcggttct gaccgtggca
     7141 ttgatcgagg ccgtggccgt aagctggttt tacggcatca cgcagttctg tagggacgtg
     7201 aaagagatgc tgggcttttc tcccggttgg ttttggagga tttgttgggt agcaattagc
     7261 cccctgtttc tccttttcat tatttgctct tttctgatgt ctccaccgca gctccgactg
     7321 tttcagtaca actaccccta ctggtcaatt aagctgggct attgtatcgg cacctcaagt
     7381 ttcatctgca tccccacata catcgcatac agactgataa ttacgcccgg tacttttaag
     7441 gagcgaatca ttaaaagcat aacccccgaa acccccaccg agatcccttg tggcgatata
     7501 agactcaatg cggtctaata gctcgaggcc ggcaaggccg gatccagaca tgataagata
     7561 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     7621 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     7681 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     7741 caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc
     7801 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     7861 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     7921 cggggcgcag ccatgaggtc gactctagag gatcgatccc cgccccggac gaactaaacc
     7981 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     8041 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     8101 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     8161 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     8221 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     8281 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     8341 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     8401 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     8461 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     8521 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     8581 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     8641 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     8701 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     8761 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     8821 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     8881 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     8941 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     9001 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     9061 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     9121 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     9181 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     9241 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     9301 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     9361 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     9421 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     9481 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     9541 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     9601 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     9661 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     9721 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     9781 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     9841 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     9901 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     9961 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
    10021 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
    10081 gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc tttgcatatg
    10141 ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc tggttgctcc
    10201 cattcttagg tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca
    10261 ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt
    10321 gacaacatgg gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga
    10381 tggccagaaa tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg
    10441 gggaatacac ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc
    10501 ccttcctcac cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc
    10561 gcggcagccc cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag
    10621 ctgcacacag tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta
    10681 atcgcatcct tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag
    10741 acaatggact cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg
    10801 agacgactca atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg
    10861 ttgtaaaggg aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg
    10921 tcggtagtcc tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc
    10981 tcaaatttcg ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg
    11041 cgcctgcctc catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct
    11101 gcggggccct gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct
    11161 tggtggtatt caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga
    11221 gggggcctgc gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc
    11281 ttttctccac gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt
    11341 tcacgtcctc taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct
    11401 cgaccccggc ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct
    11461 gctcctgccc ctcctcc
//
