LOCUS       sequence-20572-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(37..161)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(169..185)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(169..185)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(191..454)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(274..297)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 taaagtgtaa agcctggggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt
       61 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
      121 tcattttacg tttctcgttc agcttttttg tacaaacttg tgatggggga tccactagtt
      181 ctagaatccg tcgaaactaa gttctggtgt tttaaaacta aaaaaaagac taactataaa
      241 agtagaattt aagaagttta agaaatagat ttacagaatt acaatcaata cctaccgtct
      301 ttatatactt attagtcaag taggggaata atttcaggga actggtttca accttttttt
      361 tcagcttttt ccaaatcaga gagagcagaa ggtaatagaa ggtgtaagaa aatgagatag
      421 atacatgcgt gggtcaattg ccttgtgtca tcatttactc caggcaggtt gcatcactcc
      481 attgaggttg tgcccgtttt ttgcctgttt gtgcccctgt tctctgtagt tgcgctaaga
      541 gaatggacct atgaactgat ggttggtgaa gaaaacaata ttttggtgct gggattcttt
      601 ttttttctgg atgccagctt aaaaagcggg ctccattata tttagtggat gccaggaata
      661 aactgttcac ccagacacct acgat
//
