LOCUS       sequence-205731-        5061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Parental vector for making S. rosetta expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Booth DS, Szmidt-Middleton H, King N
  TITLE     Transfection of choanoflagellates illuminates their cell biology and
            the ancestry of animal septins.
  JOURNAL   Mol Biol Cell. 2018 Dec 1;29(25):3026-3038. doi: 
            10.1091/mbc.E18-08-0514. Epub 2018 Oct 3.
  PUBMED    30281390
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-08-0514"; journalName: "Mol Biol Cell"; date: 
            "2018-12-1- 1"; volume: "29"; issue: "25"; pages: "3026-3038"
COMMENT     Sequence Label: NK601
FEATURES             Location/Qualifiers
     source          1..5061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4667..4684)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4667..4683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4676..4698)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4892..4911)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5011..5033
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcctgc aggtaactca tccttgtcag catcgtttcc
     2281 tcacctcctc ctcctgtcgc tggtgcttct ggtcttgtcc agcgggaacc caaacaacaa
     2341 caacaacgaa ctaccactgc ctcggaaaat ctgcggtgat ttgcacaaat cgccaacaag
     2401 tgttgtggcg tgctaaatga gagaaacacc gtatttgagc ccaggtatcg aaatgtctgt
     2461 atggctgcat ttgtgttttc caacctgaag gaaaacagca cttgacaacg acgtttgccc
     2521 agcccgtttt tcgcggtgga tacaaatttc acctgtgtca ctgcgcatta gccccacagt
     2581 ggggcttacg aaatctacac catcccagct tccgcaccgc caaaattaac tgcgtgaaaa
     2641 aacacttgcg gcaaccacag caaccaccac aagcagccaa acactcgcaa acacccttgc
     2701 ttgcttcttg gcttttttgt agtccccttt cccttgtttt cctgttgtcg tggctgaggt
     2761 ttgcgtggcg tgttcatttt catcagtttg ctcgctcata acaagcttgc gactacctcc
     2821 cctttttggt gctgtacacc cgcgaccttt cttatcctct tctccttctc caccaccgct
     2881 caacgacaag atgcgcgaag ttctctccgt ccacattggc caggccggtt gccagatcgg
     2941 taacgcctgc tgggagctgt actgcctcga gcacggcatc caacccgatg gccagatgcc
     3001 ctctgacaag actgtcggct atggcgacga ctcgttcaac accttcttct ccgagactgg
     3061 tgctggcaag cacgtgcccc gcgccgtgtt tgtcgatctc gagcccaccg tcgtcgacga
     3121 ggtgcgcacc ggcacctacc gccagctgtt ccaccccgag cagctcatct ccggcaagga
     3181 ggacgccgcc aacaactacg cccgtggcca ctacaccgtt ggcaaggagc tcgtcgacat
     3241 tgtcctcgac cgcatccgca agctcgccga ccagtgcacc ggtctccagg gcttcctcat
     3301 cttccactcg ttcggtggcg gtaccggctc tggctttgcc tcgctcctca tggagcgcct
     3361 ctctgtcgac tacggcaaga agtccaagct cgagtttgcc gtctaccctg ctcctcaggt
     3421 gtccaccgcc gttgtcgagc cctacaactc gatcctgacg tgccacacca ccctcgagca
     3481 ctctgactgc gccttcctca tggacaacga ggccatctac gacatttgcc gtcgcaacct
     3541 cgacattgag cgccccacct acaccaacct gaaccgcctc atcgcccagg ttgtgtcctc
     3601 cgtgacggcc tccctgcgct tcgacggtgc ccttaacgtc gatctcaccg agttccagac
     3661 caacctggtg ccctaccccc gcatccactt ccctctggtg acgtacgctc ccatcatctc
     3721 tgccgagaag gcctaccacg agcagctctc cgttgccgag atcaccaact cgtgcttcga
     3781 gcccgccaac cagatggtca agtgcgaccc ccgccacggc aagtacatgg cctgctgcgt
     3841 cctctaccgc ggtgacgtcg tccccaagga cgtcaacgcc tcgattgcca ccatcaagac
     3901 caagcgcacc atccagtttg tcgactggtg ccccactggc ttcaaggttg gcatcaacta
     3961 ccagccaccc accgtcgtgc ccggcggcga ccttgccaag gtgcagcgct ccgtctgcat
     4021 gctcagcaac accaccgcca ttgccgaggc ctgggctcgt cttgaccaca agttcgacct
     4081 catgtacgcc aagcgtgcct tcgtccactg gtacgtcggt gagggtatgg aggaaggcga
     4141 gttcgccgag gcccgcgagg atcttgccgc cctcgagaag gattacgagg aggtcggcgt
     4201 cgactccatg gacggcgagg gcgaggacga gggcgaggag tactaagcgc aaggccccct
     4261 cccggcctga cctccaccac caccgtcttc actgctgcaa ttacctgcca ttgttttggc
     4321 tgtcctttga tgactggttg atgcttctgc atttgttttg tgtgtgttga gctgtctggt
     4381 ggtggcgcgt ttgttcactc ttccacccct tgcctttgct gcaagcgacc gttccatgtc
     4441 cccaaccatc caatacaaac actgaatcca acaacaattg cagcaaagag gaataagttg
     4501 tccaacagaa cgaagttatg atgagtgttg tgtgggatgt cacacatgac gcgacactgg
     4561 cagtggctgt tttgtttggt tggtggttta ctggtccgct gcgtggcggt agcgatcata
     4621 gaaggcaagc atgtctagag gatccccggg taccgagctc gaattcactg gccgtcgttt
     4681 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     4741 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4801 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     4861 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4921 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4981 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5041 cgtcatcacc gaaacgcgcg a
//
