LOCUS       sequence-205770-        5493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBA1
FEATURES             Location/Qualifiers
     source          1..5493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     153..172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     406..423
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3783..3801)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3869..3973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4195..4986
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(5157..5493,1..252)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
       61 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      121 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      181 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      241 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      301 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      361 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      421 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      481 taatcactct gtggtctcaa atgcttcaca agcgagctag tgaagctaac gataagaacg
      541 ataataccat catcggttcc gatttagcat catctaagaa acgccgtatc gatttcactg
      601 aatcttcgtc tgataagtca tcttcgattt tagcttctgg tagtagcagg ggttttcacg
      661 gcgatagcgt cgttcagcaa atcgacatgg cttttggtaa ttcgaaccgt caggagattg
      721 atgaagatct gcacagtcgc cagctcgccg tctatggtcg tgagactatg aggcgtctct
      781 ttgcttccaa tgttctcatc tcggggatgc acggtcttgg tgccgagatt gccaagaatc
      841 ttatacttgc tggtgtgaag tctgtgacct tgcatgatga aagagttgta gagctatggg
      901 acttatcaag caactttgtt ttctcggagg atgatgttgg caagaataga gcagatgctt
      961 ctgttcagaa gttacaggat cttaacaatg ctgtggttgt ctctagcttg accaaaagct
     1021 taaacaaaga ggatctttct ggcttccagg ttgttgtatt ctctgacata agcatggaaa
     1081 gagcaatcga atttgatgac tattgtcaca gccatcagcc tcctatcgct tttgtcaagg
     1141 ctgatgtcag gggtcttttc ggctctgtct tttgtgattt tgggcctgaa tttgcggttc
     1201 ttgatgttga tggagaggaa ccccatacag gcatcattgc gtctatctct aatgagaacc
     1261 aggcatttat ttcctgtgtc gacgatgaga ggcttgagtt tgaagatggt gacctagttg
     1321 ttttctcaga agttgagggt atgacagaat tgaatgatgg gaaaccaagg aagataaaaa
     1381 gtacacggcc atattcattc acccttgatg aggacactac taactatgga acatatgtga
     1441 agggtggtat tgtcactcaa gtgaaacagc caaagttact gaatttcaag ccattgaggg
     1501 aagcgcttaa ggatccaggg gattttctat ttagtgattt ctctaagttt gatcggcctc
     1561 cactccttca cttagcattc caggcacttg atcattttaa agctgaagct ggcagatttc
     1621 ctgttgctgg ctcagaagag gacgctcaga agcttatatc gattgccaca gccatcaata
     1681 ccgggcaggg tgatttaaag gtggagaatg ttgatcagaa gcttctaaga cacttttcct
     1741 ttggagccaa ggctgtcctg aatcctatgg ctgcaatgtt tggcggtatt gttggacagg
     1801 aggttgttaa agcttgctct ggaaaatttc atcctctctt ccagtttttc tactttgatt
     1861 cagtggagtc actcccctct gaacctgtgg actcgagtga ctttgcacca agaaacagcc
     1921 ggtatgatgc ccaaatatct gtatttgggg ccaagttcca gaagaaactt gaagatgcta
     1981 aagttttcac agtagggtct ggtgctcttg gctgtgagtt cttgaaaaat ttggctctga
     2041 tgggggtttc ttgtggaagc caagggaagc tgacagtgac agatgatgat atcattgaga
     2101 agagtaacct cagtcgtcaa tttctgttcc gtgactggaa cattggacag gctaaatcca
     2161 cagttgctgc ttcggctgct gctgttataa atcctcgctt caacattgag gccctgcaaa
     2221 accgtgttgg cgcggagact gagaatgtat ttgacgatgc cttctgggag aacttaactg
     2281 ttgtcgtcaa tgcactggat aatgttaatg cgaggctcta tgttgattcg aggtgcttgt
     2341 atttccagaa gcctctcctt gagtctggaa ctcttggtac aaagtgcaac acacagtcgg
     2401 ttatcccaca tctaactgaa aattacggtg cctcaaggga cccaccagag aaacaggccc
     2461 ccatgtgtac agtgcattcc ttcccgcata acattgatca ctgtttaact tgggctcgct
     2521 ctgagtttga gggtctgctg gagaaaactc ccgctgaagt gaatgcatat ctttctagcc
     2581 cagttgagta cactaactca atgatgagtg ctggtgatgc tcaggcgagg gacacattgg
     2641 agaggattgt tgagtgcctt gaaaaggaga agtgtgaaac cttccaagac tgcttaacct
     2701 gggcacgact gaggtttgag gattactttg tgaaccgtgt caagcaattg atatacacat
     2761 ttcctgaaga tgctgcgact agtaccggag ctccattctg gtctgctcca aaaagattcc
     2821 ctcgtccact ccagtactcg tcgtccgacc caagtctcct taactttatc acagcgactg
     2881 ctattttacg agcagagaca tttgggatcc cgatacctga gtggactaag aacccaaagg
     2941 aagcagcaga agctgttgac agagtgatag tcccagactt tgagccaagg caagatgcaa
     3001 agattgtgac tgatgagaaa gctaccactt taaccactgc ttcggtggac gacgctgcag
     3061 tcatcgacga tctcattgct aagatcgacc agtgtaggca taacttgtcg ccagacttca
     3121 ggatgaaacc tattcagttc gaaaaggatg atgatacaaa ctatcacatg gatgtgatag
     3181 caggactagc caacatgagg gcgagaaatt acagcatccc tgaagtggac aagctgaaag
     3241 caaagttcat agcagggaga atcatcccag ccattgcgac ttcaacagcc atggctactg
     3301 ggctggtctg cctcgagctg tacaaggtcc ttgatggagg acataaagtg gaagcttaca
     3361 gaaacacatt tgccaacctg gcacttccac tattctccat ggctgaacca cttccaccaa
     3421 aggtggtgaa gcaccgtgac atggcttgga cagtttggga cagatgggtt ctcaagggaa
     3481 acccaactct tcgtgaggtg ttgcagtggc tggaggacaa agggcttagc gcatacagca
     3541 tctcttgtgg aagctgtcta ctgttcaaca gtatgttcac aaggcacaag gagaggatgg
     3601 acaagaaggt tgtggatctc gctcgggatg ttgcgaaagt cgagttaccg ccttatcgca
     3661 accatcttga tgtagtggtg gcttgtgagg atgaagatga caatgacgtc gatattcctc
     3721 tcgtctctat ctacttcagg tgagctttga gaccacgaag tggctcttca gtggacgaaa
     3781 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     3841 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3901 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3961 aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     4021 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct
     4081 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     4141 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg
     4201 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     4261 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     4321 aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     4381 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     4441 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     4501 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     4561 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     4621 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     4681 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     4741 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     4801 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     4861 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     4921 gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc
     4981 aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5041 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5281 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5341 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5401 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5461 cagcggtcgg gctgaacggg gggttcgtgc aca
//
